[ccp4bb] Difference between different b factors values

Dear all I request you to please tell me the difference between wilson B-factor and average B, all atoms ?? I have two structures one native at 2A resolution, having mean b factor of 24 and the other a complex structure of the same protein with a ligand (at 2.6A resolution) has mean b factor of

[ccp4bb] AW: [ccp4bb] Difference between different b factors values

Auftrag von Faisal Tarique Gesendet: Donnerstag, 14. August 2014 10:31 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] Difference between different b factors values Dear all I request you to please tell me the difference between wilson B-factor and average B, all atoms ?? I have two structures one native

Re: [ccp4bb] Difference between different b factors values

Dear Faisal, at 2.6A resolution I would not call 23A^2 and 24A^2 'a difference', rather a fluctuation. Parameters are not totally independent from each other and not from experimental issues. It most likely also make a difference which program you use. Some program has a upper cut-off of 100-200