o wrote:
>> Thank you Kevin,
>>
>> I will try to remove b-ME as you suggested.
>>
>> Min-Kyu
>>
>> | -----Original Message-----
>> | From: Kevin Jin [mailto:kevin...@gmail.com]
>> | Sent: Monday, March 12, 2012 5:50 PM
>> | To: Min-Kyu Cho
>&
PM
> | To: Min-Kyu Cho
> | Subject: Re: [ccp4bb] My protein precipitates at r.t and dissolves at 4
> | oC
> |
> | Hi Min,
> |
> | I need to look back my note. Here is from my old memory:
> |
> | 1. The protein was loaded in FPLC column at 4 degree C and the collectio
] My protein precipitates at r.t and dissolves at 4
| oC
|
| I remember I saw the similar problem caused by beta-mercaptoethanol.
|
|
| Kevin
|
|
| On Mon, Mar 12, 2012 at 1:29 PM, Artem Evdokimov
| wrote:
| > Could be one of those weird behaviors displayed by detergents wh
gt;>
>> Min-Kyu
>>
>> | -Original Message-
>> | From: Kevin Jin [mailto:kevin...@gmail.com]
>> | Sent: Monday, March 12, 2012 11:16 AM
>> | To: Min-Kyu Cho
>> | Cc: CCP4BB@jiscmail.ac.uk
>> | Subject: Re: [ccp4bb] My protein precipitates
gt; | Subject: Re: [ccp4bb] My protein precipitates at r.t and dissolves at 4
> | oC
> |
> | Which kind of buffer you use? If it is Tris, then temperature change
> will
> | cause pH change.
> |
> | Actually, this is a good way for crystallization.
> |
> | Kevin
Min-Kyu,
This sounds like to be a hydrophobicity ~ temperature issue (as Kevin pointed
out, pH gets involved too).
If your protein is sensitive in this regards, it could form different
oligomerization states at diff temp, associated
with diff solubility. You may want to crystallize it at diff
AM
| To: Min-Kyu Cho
| Cc: CCP4BB@jiscmail.ac.uk
| Subject: Re: [ccp4bb] My protein precipitates at r.t and dissolves at 4
| oC
|
| Which kind of buffer you use? If it is Tris, then temperature change will
| cause pH change.
|
| Actually, this is a good way for crystallization
Which kind of buffer you use? If it is Tris, then temperature change
will cause pH change.
Actually, this is a good way for crystallization.
Kevin
On Mon, Mar 12, 2012 at 9:02 AM, Min-Kyu Cho wrote:
> Hi all,
>
> I have a homotetrameric coiled-coil domain sample with 45aa per each. While
> I st
Hi all,
I have a homotetrameric coiled-coil domain sample with 45aa per each.
While I store this sample at 4oC, the sample looks clear w/o any
particles. But when I took out the sample to my bench at r.t, I can see
there are precipitates (as stack of needle like particles) at the bottom
of
