Subject: [ccp4bb] control of nucleation
hello,everybody . due to excess nucleation,I often get many tiny
crystals instead of few,large crystals.i wana optimize the condition,
does anyone have adivce about this?
Best regards.
hello,everybody . due to excess nucleation,I often get many tiny crystals
instead of few,large crystals.i wana optimize the condition, does anyone
have adivce about this?
Best regards.
Dear zq deng,
The standard method, I dare say, in such a case is micro seeding:
reduce the precipitant to a concentration so that you just do not get crystals
any more, wait until the drop equilibrates (about one day in the case your
precipitant is a salt, up to 5-7 days for high MW PEGs),
then
Roszak
From: zq deng dengzq1...@gmail.com
Reply-To: zq deng dengzq1...@gmail.com
Date: Thu, 6 May 2010 09:03:46 +0100
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] control of nucleation
hello,everybody . due to excess nucleation,I often get many tiny crystals
From: zq deng dengzq1...@gmail.com Reply-To: zq deng dengzq1...@gmail.com Date: Thu, 6 May 2010 09:03:46 +0100 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] control of nucleation hello,everybody . due to excess nucleation,I often get many tiny crystals instead of few,large crystals.i wana optimize the c
Dear Zq Deng,
I've had success with the dilution method as described
by Dunlop and Hazes: http://scripts.iucr.org/cgi-bin/paper?en5016
For me it worked for a couple of projects, and gives you a bit more to
permutate than just varying the protein:mother liquor ratios, as Thomas
I mistakenly sent this off to Enrico, rather than to the CCP4BB. Apologies to
Enrico.
Begin forwarded message:
From: Charles W. Carter, Jr car...@med.unc.edu
Date: May 6, 2010 6:50:23 AM EDT
To: est...@cea.fr
Subject: Re: [ccp4bb] control of nucleation
In fact, there is quite good
] control of nucleation
To: CCP4BB@JISCMAIL.AC.UK
Date: Thursday, May 6, 2010, 1:33 PM
hello,everybody . due to excess nucleation,I often get many tiny crystals
instead of few,large crystals.i wana optimize the condition, does anyone have
adivce about this?
Best regards.
by using oil (such as parafin oil ... etc ) at reservoir
on top of the reservoir solution. You have to try several trials to find
optimum ratio.
With regards
Syed
--- On *Thu, 5/6/10, zq deng dengzq1...@gmail.com* wrote:
From: zq deng dengzq1...@gmail.com
Subject: [ccp4bb] control
