Re: [ccp4bb] process SeMet labelled data

2007-03-01 Thread Miguel Ortiz Lombardia
-BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Dear ccp4bbers, I agree with Dirk. I have also noticed that much due to the way X-ray crystallography is evolving, a lot of students/early-postdocs find themselves doing crystallography in labs without a tradition in crystallography, even without

[ccp4bb] Swiss humour - no laughing matter? (Re: [ccp4bb] process SeMet labelled data)

2007-03-01 Thread Gerard DVD Kleywegt
gruzi dirk, come on - lighten up a little! your reply wasn't funny or helpful either - just moralising. (by the way - 't is a strange fact that whenever i get any negative reactions to my own postings they stem *exclusively* from any or all of these three countries: the us, germany and

Re: [ccp4bb] Swiss humour - no laughing matter? (Re: [ccp4bb] process SeMet labelled data)

2007-03-01 Thread James Stroud
I have found in my usenet adventures that usenet mavens often point to this link before trying to sort out among themselves exactly what level of humor to take with dubious posts: http://www.catb.org/~esr/faqs/smart-questions.html Of course usenet mavens also complain a little too

Re: [ccp4bb] process SeMet labelled data

2007-03-01 Thread James Whisstock
Dear Shivesh Below I've tried to give you some broad ideas about where to look / read and some packages / approaches to try. For a start there is a very comprehensive tutorial on the ccp4 site together with the experiemental phasing roadmaps.

Re: [ccp4bb] Swiss humour - no laughing matter? (Re: [ccp4bb] process SeMet labelled data)

2007-03-01 Thread Dirk Kostrewa
Goede dag Gerard, interesting that you think my last e-mail was addressed to you ;-)! Yes, this was my first moralizing posting on this board after some 16 years. A bit sense of humor is fine, but the last posting was simply too much. We shouldn't attack people personally or discriminate

Re: [ccp4bb] process SeMet labelled data

2007-03-01 Thread Eleanor Dodson
shivesh kumar wrote: Dear all, I have a data set at 2.2A, of the selenomethionene labelled protein.How should I process the data.Thanx for the help. Shivesh I think you should ask your local crystallographer for help. There are several programs ( MOSFLM in CCP4, DENZO, XDS, D*TREK ) but

[ccp4bb] Fwd: [ccp4bb] Swiss humour - no laughing matter? (Re: [ccp4bb] process SeMet labelled data)

2007-03-01 Thread Klaus Piontek
board CCP4BB@JISCMAIL.AC.UK From: Gerard DVD Kleywegt [EMAIL PROTECTED] Subject: [ccp4bb] Swiss humour - no laughing matter? (Re: [ccp4bb] process SeMet labelled data) Comments: To: Dirk Kostrewa [EMAIL PROTECTED] To: CCP4BB@JISCMAIL.AC.UK List-Help: http://www.jiscmail.ac.uk

Re: [ccp4bb] process SeMet labelled data

2007-02-28 Thread Petrus H Zwart
Dear all, I have a data set at 2.2A, of the selenomethionene labelled protein.How should I process the data. Properly

Re: [ccp4bb] process SeMet labelled data

2007-02-28 Thread Jonathan Grimes
Anastassis Perrakis wrote: On Feb 28, 2007, at 14:37, shivesh kumar wrote: Dear all, I have a data set at 2.2A, of the selenomethionene labelled protein.How should I process the data. Carefully ! Thanx for the help. Shivesh Tassos i am sure what tassos really meant was Very

Re: [ccp4bb] process SeMet labelled data

2007-02-28 Thread Clemens Vonrhein
On Feb 28, 2007, at 14:37, shivesh kumar wrote: Dear all, I have a data set at 2.2A, of the selenomethionene labelled protein.How should I process the data. Some hopefully useful remarks (fairly random and not complete and exhaustive): 1. make sure to mask out the backstop and beamstop holder

Re: [ccp4bb] process SeMet labelled data

2007-02-28 Thread Mark J. van Raaij
why don't you just send all your images to the ccp4bb, then we'll process them, solve the structure and publish it for you. And we might put you in the acknowledgements, if you are lucky. Mark On 28 Feb 2007, at 16:35, Jonathan Grimes wrote: Anastassis Perrakis wrote: On Feb 28, 2007, at