Hi,
Temperature is one obvious factor that comes to mind.
If you have access to MS, you could check the protein from the
crystallization drops - find out if there's something 'chemical' going on
such as proteolysis, modification of some sort, etc. - this has happened
to me once before, the
On Mon, 2007-08-06 at 15:50 -0400, Neeraj wrote:
hi all,
I am working on a protein for which we get nicely diffracting
crystals but the problem is that the crystals grow from anywhere between
4-6 months. Does anyone has any general suggestions as to what things
can be changed or
Hi Neeraj,
There are several ways to approach this problem. Of course, this also
depends on your sample and the amount you have available.
1. Probably the first thing to try is seeding (streak or micro).
2. You could try with higher protein and/or precipitant concentration.
3. If you have enough
Hi Neeraj,
Two more tricks:
1. Open the cover slip a bit to allow for faster evaporation. It
worked well on one of my proteins.
2. in-situ proteolysis: Acta Crystallograph Sect F Struct Biol Cryst
Commun. 2006 Oct 1;62(Pt 10):1041-5.
Good luck.
Zhen
Quoting Neeraj [EMAIL PROTECTED]:
hi all,
