[ccp4bb] Postdoctoral researcher position in Structural Biology at Uppsala University

Dear all, I have an opening for a postdoctoral researcher in my group at the Department of Cell and Molecular Biology, Uppsala University. The project aims to study the structure and function of metal-responsive transcription factors. The successful candidate should have an excellent track

Re: [ccp4bb] Anderson?Evans polyoxotungstate (TEW)

We bought some recently hoping for some magic for a specific project that we needed new crystal forms for.  Alas, no fairy dust magic in our hands.  Maybe we didn't do the protocol right - but of course, one doesn't expect to have to do that with fairy dust. We haven't tried with many things,

[ccp4bb] SGC Oxford Symposium: Exploiting Disease Genomics to Catalyse New Medicines; June 14th

[cid:image001.png@01D3C766.E1037C50] We're pleased to announce that registration is now open for SGC Oxford's upcoming symposium "Exploiting Disease Genomics to Catalyse New Medicines" which will take place at St. Catherine's College, Oxford on 14th June 2018. Full details and links for

Re: [ccp4bb] Sulphur SAD at home source

Iodine is ideally suited for Cu K-alpha SAD phasing, and iodide ions can normally be easily added by soaking crystals in potassium iodide containing solutions, which can be done at the time of cryopreservation. A quick lit search will turn up the appropriate protocols. For structural genomics

Re: [ccp4bb] Sulphur SAD at home source

Hi Manoj, Like Eleanor has pointed out a very accurate data may get you a solution. If you have crystals that diffract well and give you data with high I/sigma(I) you can attempt to find the positions of the S atoms and use that to arrive at a structure. Using lysozyme as a test case we have

[ccp4bb] Sulphur SAD at home source

Hi All, I am writing to seek advice on doing sulphur SAD data collection at Cu based home source for a protein that is 12 KDa and has 6 S atoms. I have seen some links online and some references but would be grateful if you can share your know-how for success with this. Like what multiplicity of

Re: [ccp4bb] Sulphur SAD at home source

Dear Manoj, providing your crystals diffract to at least 2.5 Å and the sulfurs are ordered, you should be able to solve your structure without too many problems. As a first experiment, I would recommend collecting 360 degrees of data. Process these with XDS and the FRIEDEL'S_LAW= FALSE option

Re: [ccp4bb] Sulphur SAD at home source

Jim Pflugrath and a couple of high school interns shot a short video on halide quick soaks a few years back. You can find it at https://www.youtube.com/watch?v=45Qc3jOPaKY. Cheers, Joseph D. Ferrara, Ph.D. CSO Deputy Director, X-ray Research Laboratory Vice President, American Crystallographic

Re: [ccp4bb] Sulphur SAD at home source

Well - the S f" is only ~ 0.5 at Cu Kalpha so the signal will be very weak.. Very accurate data may get a solution but you first have to position the S atoms... Much easier to try to make a heavy atom derivative! Eleanor On 3 April 2018 at 15:26, Manoj Saxena <

Re: [ccp4bb] Sulphur SAD at home source

Dear Manoj, I'm happy to answer your questions and have a look at your data. Maybe we can take this off-list and you report back if and when you solve your structure. By the way, I'm processing native data collected with a MetalJet at the moment. The anomalous signal is even weaker at 1.35 Å