Huiying Li wrote:
I tried to scale, using SCALA through CCP4i GUI, three blocks of data
collected with one crystal (3 mtz files output from MOSFLM). The GUI
has only one MTZ input slot. Which program can be used to combine the
3 unmerged mtz files together? CAD refused to handle these raw mtz f
We have been quite happy with our crystallization robot from Innovadyne. It
transfers from deep well blocks to crystallization plates, and very reliably
sets up 200nl + 200nl drops. We have tried smaller drops with decent
success, which works better with certain plates. It can be easily
programmed,
I tried to scale, using SCALA through CCP4i GUI, three blocks of data
collected with one crystal (3 mtz files output from MOSFLM). The GUI has
only one MTZ input slot. Which program can be used to combine the 3
unmerged mtz files together? CAD refused to handle these raw mtz files.
Thanks in a
Eleanor Dodson wrote:
> It should be more or less equivalent, but better I think to put 1S at
> 0.5 occ and 2O at occ = 1
>
> At least in REFMAC the restraints to the symmetry atoms should be set up
But if you do that, some of the bond angles will be unrestrained, won't
they?: O1-S-O2 will be OK,
What a great time in crystallization; lots of options, lots of choices.
After reviewing the automation available for crystallization and our unique
needs we decided on the Phoenix. We have used the Phoenix from Art Robbins
Instruments with excellent results since installation October 2006.
Eac
All,
I am posting on behalf of Dr. Karolin Luger. Dr. Luger has a job opening for a
post-doctoral researcher.
Job Summary:
Looking for a highly motivated individual with a strong interest in
integrated approaches to problems in structural biology. The lab has
extensive crystallographic and sp
I bought a replacement Cartesian Tip (in Australia) on the 8th of
october, 2007 and got charged $660 AUD (of which 60 bucks was the GST
tax) - on the 8th of october, the AUD-USD exchange rate was 0.8956, so
that would be $537 USD, which is quite a long way from both '$700' and
'under $300'.
The t
This is an additional reminder to the community that January 12 (Saturday)
is the deadline for abstract submissions for the New Structures session at
the 2008 ACA meeting in Knoxville, TN.
The session will feature a balance of talks from both the protein and
nucleic acid crystallographic communi
Postdoctoral positions in Structural Biology are available immediately
in the Chen laboratory at Purdue University. As part of the Markey
Center for Structural Biology, the Chen lab integrates X-ray
crystallography and biochemical methods to study the mechanism of active
transport (See Nature, 450
Dirk Kostrewa wrote:
Am 10.01.2008 um 01:53 schrieb Dale Tronrud:
...
Your quote bring up another matter. An "initial" map, i.e. before
refinement, is unbiased if it is an omit map. If you have done no
refinement and you leave the interesting part out of the calculation
of Fc there can not
Phoebe,
We were able to purify the estrogen receptor DNA binding domain with a
6his-tag. After cutting off the tag and re-applying to the Ni-NTA matrix,
the protein did not stick to the beads. We had some low resolution crystals
that contained a second protein and DNA, which suggests to me that th
Dear colleagues,
We are pleased to announce that the Ligand Recognition and Molecular
Gating GRC will be held in Ventura, CA, 2-7 March 2008.
This Gordon Research Conference focuses on the structure and function of
integral membrane proteins involved in transmembrane signaling
(receptors) an
I was wrong on the price- yes $300/per, but still, it was a lot of money
when we had catastrophic crash several years ago (due to something going
haywire- not a script or operator error). I know the nitonol tips are
not fool-proof- they just seem a bit more forgiving than the ceramic
tips.
As
We have recently opened up two postdoc positions, one in bioinformatics
and one in crystallization. The descriptions can be found here:
http://www.sgxpharma.com/careers/opportunities.php
They read in part,
Bioinformatics position:
"The candidate will perform original basic research in structural
Hi Buz,
> Does anyone have a python routine that can be used to calculate the
> moment of inertia matrix and principal axes of a selection of atoms?
If you have Phenix, CCI Apps or just cctbx installed, try the script below.
Example:
mmtbx.python script.py 1NSF.pdb 'resname GLU and resid 73
With regard to the Phoenix:
I'm not sure why you've had these problems with the robot,
We use 100+100ul regularly and the CryCam has little or no problem scanning
in the drops. Maybe it needs a little TLC to get it properly aligned.
12mins seems a little too long for a 3x96 plate - I'm sure with
A little comment on not breakable Phoenix needles.
I saw not recoverably bended tips (all 96!) as a result of not very
careful operation (done by rep) during presentation of the instrument, I
do not know how much is 96 nitinol tips set for replacement
Additionally, you hardly can go with 100+
Not to inject a commercial tone, but there seems to be a bias in the
posting below. The ceramic tips are breakable, but not all find them
incredibly so. And the price is less then $300, not $700.
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
Lisa A Na
Please do NOT use the BB for abuse.
thank you for your support. although i think some abuse of the english
language is unavoidable in an international forum like this
--gerard
(more important issue: http://www.irrepressible.info/)
Am 10.01.2008 um 01:53 schrieb Dale Tronrud:
...
Your quote bring up another matter. An "initial" map, i.e. before
refinement, is unbiased if it is an omit map. If you have done no
refinement and you leave the interesting part out of the calculation
of Fc there can not be any bias in either
Am 09.01.2008 um 20:48 schrieb Anastassis Perrakis:
I actually think that inaccurate cells are a big source of misery
in many refinements. I have found the idea
of WhatCheck to actually check your cell by looking at the
projection of bond lengths of certain types along the cell axes
most us
Jie Liu wrote:
> Dear all
>
> I have a sulfate ion sitting on a 2-fold axis. Should I put in pdb file
> one S atom with occu=0.5 and two O atoms with occu=1, or should
> I put one S and four O atoms all with occu=0.5?
>
> Thanks for your inputs.
>
> Jie
>
>
>
It should be more or less equivalent
Please do NOT use the BB for abuse.
Eleanor
Gerard DVD Kleywegt wrote:
Be care, you insolence might get someone sent disappear.
is the chinese secret service editing my incoming mail already, or are
you merely using a microsoft grammar checker?
--dvd
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