Happen to come across this:
Failed to rerun previous refmac job, which had been run in version
5.5.0109. The problem was gone after changing the LIBIN filename (36
characters) to a shorter one.
Minglei Zhao
Dear Zhihong,
Refinement stuck at 55% Rfree (which is essentially random), means that
you do not have found the correct MR solution. For me the prime suspect
is the space group. In my experience pseudo translation or any almost
crystallographic NCS will easily confuse automatic space group
Dear All,
I am trying to run MolRep with 'input fixed model' option and i am getting
error message:
At line 1260 of file /usr/local/xtal/ccp4-6.2.0/src/molrep_/molrep.f
Fortran runtime error: End of record
I am using:
CCP4 6.2: MOLREP(ccp4) version 11.0.02 : 07/02/11
I found the
you can try a version from this site:
http://www.ysbl.york.ac.uk/~alexei/molrep.html
If it does not work please let me know
regards
Garib
On 15 Mar 2012, at 12:45, Justyna Wojdyla jw...@york.ac.uk wrote:
Dear All,
I am trying to run MolRep with 'input fixed model' option and i am
Dear all,
Is there any similar function in COOT as lego_auto_mainchain command in
O program? This command is quite useful to build a low resolution model
(say, 4A).
Cheers,
Xiuwen
On 15/03/12 14:14, xiuwen zhang wrote:
Dear all,
Is there any similar function in COOT as lego_auto_mainchain
command in O program? This command is quite useful to build a low
resolution model (say, 4A).
I am not familiar with lego_auto_mainchain but there are 2 loop fitting
tools in
Dear BB,
Apologies for being mildly off topic.
Maybe.
1. We are trying to express (in E. coli) a protein which appears to be quite
sensitive to mechanical disruption. We have ordered some B-PER (Pierce - B-PER
Bacterial Protein Extraction Reagents are designed to extract soluble protein
Switch ethanol in the stain. Also, there was talk a while ago about making
your own instant blue stains--it works like bradford reagent, I think.
Perhaps look back at previous postings?
JPK
On Thu, Mar 15, 2012 at 9:24 AM, Thomas Edwards t.a.edwa...@leeds.ac.ukwrote:
Dear BB,
Apologies for
Hi Ed,
A few years back there has been a thread on copper staining that leads
to negative staining, but works well, is very fast and simple.
- Rinse gel 10-30s in H2O
- incubate in 300 mM CuCl2. The gel stains in 3-5 min: background
becomes opaque, the protein bands remain clear. Sensibility
By mechanical disruption you mean sonication only or have you tried the
French press?
Assuming that you use sonication, and assuming that you follow a fairly
standard protocol (e.g. something like 10sec pulse/20sec pause on ice
for 3 minutes total), it may be heat not ultrasound that gets to it.
Hey, that was my post! NB this cannot be used for native gels, I don't
think.
Jacob
On Thu, Mar 15, 2012 at 9:47 AM, Cécile Breyton cecile.brey...@ibs.frwrote:
Hi Ed,
A few years back there has been a thread on copper staining that leads to
negative staining, but works well, is very fast
Hi all,
concerning the staining, we get good results with a self-made instant blue.
First staining is achieved within 30min, full staining after maximal 4 hours I
would say. You don't need to destain (still possible with H2O) since the
background is not stained. Sensitivity is better than
This is a reminder that applications are now open for the
BCA/CCP4 Summer School in Protein Crystallography to be held at
Diamond (UK) from Tuesday 28th August to Sunday 2nd September 2012.
Applications close 1st May 2012.
The course is suitable for PhD students in macromolecular
Dear colleagues
do you have any professional thoughts on my case here?
Available SeMet data to 3.6 Å (where only the redundant Se-Edge peak to can
be used), a high resolution native data to 2.6 Å, a
Zinc-soak, collected at the Zinc-Edge to 3.0 Å.
Considerable Patterson peak indicates pseudo
Hi all.
I set up some trays of membrane protein remotely in cubic phase. I don't have
ready access to them so I can't shoot/pick the crystals. Under polarising
lights, some crystals appears coloured across many conditions, making me think
these are salt. Is there some knowledge of inorganic
Hi Theresa,
Your observation of colored crystals under polarising lights seems odd to
me. I assume your protein should be colorless and under normal light these
crystals are colorless? Are these trays set up in glass plate or plastic
plates? If you are willing to upload some pictures, I might be
The best reproduction I can suggest would be to setup one or two LCP
experiments exchanging the protein for its buffer. If you get crystals, you
know for sure they're not protein.
Cheers,
Jon
2012/3/15 Theresa H. Hsu theresah...@live.com
Hi all.
I set up some trays of membrane protein
Dear Elias,
we routinely produce a C-terminally His6-tagged catalytic domain of bovine
enterokinase in E. coli. The protease is expressed into inclusion bodies and
subsequently folded in vitro. Considering the high activity of our enterokinase
construct, one refolding batch allows us to cleave
On Thu, Mar 15, 2012 at 11:14 AM, Jon Agirre jon.agi...@gmail.com wrote:
The best reproduction I can suggest would be to setup one or two LCP
experiments exchanging the protein for its buffer. If you get crystals, you
know for sure they're not protein.
It should be pointed out that the
A post-doctoral position is available for highly motivated candidates to join a
research group interested in investigating molecular mechanisms underlying
epigenetic regulation and inheritance, and the broad area of chromatin biology.
Projects will involve structure determination of
A post-doctoral position is available for highly motivated candidates to
join aresearch group interested in investigating molecular mechanisms
underlyingepigenetic regulation and inheritance, and the broad area of
chromatin biology.Projects will involve structure determination of
macromolecular
1. We like Bugbuster. You can get it at 10X concentration so it is
fairly economical. We do add lysozyme and DNase as well.
2. We use the NuPage gels and SimplyBlue SafeStain and it works very
well. We can run a gel in 20-30 min and have it stained and destained a hour
later using the
Hi all,
Has any of you encountered a protein with 4Fe-4S cluster; with three
of the Fe coordinated to the cysteines of the protein, leaving one Fe
atom free for electron transfer?
The closest I got is aconitase; however, the iron sulfur cluster of
the enzyme is not used for electron
Sorry for posting again, previous message contains a typo.
A post-doctoral position is available for highly motivated candidates to join a
research group interested in investigating molecular mechanisms underlying
epigenetic regulation and inheritance, and the broad area of chromatin biology.
24 matches
Mail list logo
