Re: [ccp4bb] protein crystals or salt crystals

2013-02-07 Thread Frank von Delft
Test the diffraction - that's the only way. But given the other junk in the drop, chances are they're salt. (And don't post 5Mb attachments, please.) On 07/02/2013 22:24, amro selem wrote: Hallo my colleagues. i hope every one doing ok . i did screening since two weeks . i noticed toda

[ccp4bb] Molecular cloning software for linux

2013-02-07 Thread Theresa Hsu
Dear all Is there any good molecular cloning software for linux? It should read plasmid constructs in Genbank format, do assembly of plasmid sequencing results, display the sequencing trace and highlight regions where there are mutations based on relative QV values, has a list of built-in restr

[ccp4bb] Group Leader- Structurall Biology - Protein Chemistry Opportunity

2013-02-07 Thread jovanna bost
Position: Group Leader– Structural Biology – Protein Chemistry   Client: Dart NeuroScience LLC (DNS) is a privately held Delaware Limited Liability Company with offices in San Diego, CA.  DNS will discover new technologies and develop new therapies to help maintain cognitive vitality throughout lif

[ccp4bb] Group Leader- Structural Biology- Protein Production Opportunity

2013-02-07 Thread jovanna bost
Position: Group Leader – Structural Biology – Protein Production   Client: Dart NeuroScience LLC (DNS) is a privately held Delaware Limited Liability Company with offices in San Diego, CA.  DNS will discover new technologies and develop new therapies to help maintain cognitive vitality throughout l

[ccp4bb] Group Leader- Structural Biology - Crystallography Opportunity

2013-02-07 Thread jovanna bost
Position: Group Leader – Structural Biology – Crystallography   Client: Dart NeuroScience LLC (DNS) is a privately held Delaware Limited Liability Company with offices in San Diego, CA.  DNS will discover new technologies and develop new therapies to help maintain cognitive vitality throughout l

[ccp4bb] Group Leader- Structural Biology - Crystallography

2013-02-07 Thread jovanna bost
  Position: Group Leader – Structural Biology – Crystallography   Client:Dart NeuroScience LLC (DNS) is a privately held Delaware Limited Liability Company with offices in San Diego, CA.  DNS will discover new technologies and develop new therapies to help maintain cognitive vitality throughout lif

Re: [ccp4bb] protein crystals or salt crystals

2013-02-07 Thread Nat Echols
If SPG buffer is what I think it is, that means you have a significant concentration of inorganic phosphate, which forms salt crystals when mixed with divalent metal ions. -Nat On Thu, Feb 7, 2013 at 2:24 PM, amro selem wrote: > > > > > Hallo my colleagues. > i hope every one doing ok . i did s

Re: [ccp4bb] Off Topic- Cystine Detection

2013-02-07 Thread Jacob Keller
Even so, it should run somewhat differently, and probably visibly on SDS-PAGE. Even a single phosphorylation changes mobility, and I think I remember having seen intramolecular disulfides change mobility (anyone else have experience on this?) JPK On Wed, Feb 6, 2013 at 10:33 PM, Yuri wrote: > *

Re: [ccp4bb] Off Topic- Cystine Detection

2013-02-07 Thread Nadir T. Mrabet
Perhaps you should have a look at http://193.146.160.29/gtb/sod/usu/$UBUG/repositorio/10320076_Hawkins.pdf. and at "An introduction to methods for analyzing thiols and disulfides: Reactions, reagents, and practical considerations. Rosa E. Hansen 1, Jakob R. Winther Analytical Biochemistry 394 (2

Re: [ccp4bb] Pilatus 300K CBF to Oxford Diffraction format convert

2013-02-07 Thread Graeme Winter
Hi Folks, To follow up Harry's comments. We have a Pilatus 300K on I19 here at Diamond which is used from time to time for small molecule work. Processing with XDS has proved to be successful (I can send an example XDS.INP but you will need to make sure you have a good knowledge of the experimenta

Re: [ccp4bb] COOT v. 0.7

2013-02-07 Thread Gary Battle
Dear Xavier, We have put redirects in place for the PDBe download links use in Coot. e.g. http://www.ebi.ac.uk/msd-srv/oca/oca-bin/save-pdb?id=4AW6 now redirects to www.ebi.ac.uk/pdbe-srv/view/files/4aw6.ent You should be able to download PDB files without any problems. Best regards, Gary.

[ccp4bb] Register now! 11th International Conference on Biological Synchrotron Radiation (BSR), Hamburg, Germany, 8-11 Sept 2013

2013-02-07 Thread Rosemary Wilson
*REGISTER NOW!!!* *Early bird registration deadline is 1st March 2013!* *11th International Conference on Biological Synchrotron Radiation (BSR)* *Hamburg, Germany* *8th - 11th September 2013* The conference aims to bring together scientists involved in the methodical developments on synchrotro