Do you have evidence that the oil blocks diffusion of O2? O2 is a nonpolar
molecule, generally much more soluble in oils than in water. I'm not sure about
silicone oils, but I would think they also dissolve O2 readily.
eab
On 03/18/2015 08:02 AM, Patrick Shaw Stewart wrote:
Hi Steve
I have
Hi Steve
I have one more comment for this thread.
The microbatch-under-oil method is very handy for anaerobic work:
1. You can keep the microbatch stock solutions in normal microtitre plates
(polypropylene is best to reduce evaporation) for months, which hugely
reduces the amount of degassing
Hi Mirek,
I have to reinstall xquartz to get my ccp4 and coot working after upgrading
to Yosemite from Mavericks.
Xiao
On Tue, Mar 17, 2015 at 4:30 PM, Cygler, Miroslaw miroslaw.cyg...@usask.ca
wrote:
Hi,
I am thinking of upgrading the os on my mac to Yosemite. Are there any
known issues
Project description
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The situation becomes particularly severe when weeds become resistant to
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Hi Marc,
I have a Macbook pro Mid 2009 model (with integrated graphics card) and I
can run coot and pymol without any problem ( I haven't tested on Chimera),
I do not think a discrete graphics is needed. However, for pymol I can not
use the stereo function. I do not know if the discrete graphic
Hi,
As far as I can tell oil does not block diffusion of O2 whatsoever. You
can keep larger volumes (≥1 ml) of solutions anoxic in air for several
hours with dithionite (≥0.5%) to scavenge oxygen and a redox indicator
dye such as phenosafranin to monitor the state of the solution. Small
Hi,
I just checked the PIC sever tim suggested. very nice indeed. If
you only want to map different interfaces and the amino acids involved
in, I suggest to run the pisa server, too. http://www.ebi.ac.uk/pdbe/pisa/ . I
used it extensively to find out whether a certain set of crystal contacs
I also wondered about the statement about oils blocking diffusion of O2. We
had lots of trouble keeping things anaerobic in a glove box until we degassed
the oils and waxes used to mount crystals in capillaries. We found that
putting them under vacuum removed much of the dissolved oxygen.
It's a little complicated. It's true that oxygen is more soluble in most oils
than in water - but in a high viscosity mineral oil the diffusion rate is
orders of magnitude lower. So the combination of an oil overlay and a reducing
agent in your buffer should protect your sample much longer than
(Reposting this position as it didnt go to the jobs list..:)
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Hello CCP4,
Sorry for the labware related post, but I think this is the right audience.
I have 4 new reels of mosquito tips with 4.5mm pitch (for 384 well setups,
TTP labtech part number 4150-03010). I only use 96well format, thus am
looking to swap these for 9mm pitch (part number 4150-03020).
Actually I may have misunderstood the original post. Patrick never said oils
block O2 diffusion:
On 03/18/2015 09:47 AM, Edward A. Berry wrote:
The microbatch-under-oil method is very handy for anaerobic work:
(In a glove box, of course)
1. You can keep the microbatch
Hello Marc,
obvious differences between card are memory bandwidth, intel 13 GB/sec
vs. nvidia 29 GB/s, the texture rate, 2 GTexel/s vs. 31 GTexel/s, and
pixel rate, 0.5 GPix/s vs. 15.5 GPix/s. Some of these values result
because of graphics memory differences. There is no difference in
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