[ccp4bb] Off-topic: X-ray equipment for grab. MAR345, Xenocs-mirror, Oxford cryo system and an RU-H3R etc.

Hi all, I have my home source X-ray equipment up for grab. I used all the equipment one month ago and collected a beautiful 1.9Å data on a nuclear receptor LBD so everything is in working order, but I give no garantees. It is yours if you come and pack and ship it yourselves. If anyone wants

Re: [ccp4bb] Alexander Rich

Dear Colleagues, I do not think it is highly inappropriate that a crucial episode in the history of crystallography is described/commmented on in this thread, and I am not sure by whom its removal will be highly appreciated, other than by Prof. Berger. I do not know any of the protagonists of

[ccp4bb] Scientific Computing in Structural Biology - Support Engineer - SCB Unit, EMBL Heidelberg

Dear Colleagues, The Structural and Computational Biology Unit at EMBL Heidelberg, Germany is looking to recruit an Engineer for Scientific Computing in Structural Biology to handle the following responsibilities: - Development and support of the scientific computing environment of the

[ccp4bb] calculation of ASA between two helices

Hi all, I am trying to calculate accessible surface area between 2 helices from 2 different domains of protein. PISA gives me either total interface of the protein or per residue. Can anyone please suggest me what would be the best possible way to get it? Thanks and regards Atul

Re: [ccp4bb] calculation of ASA between two helices

How about cutting up the pdb file into the desired sections and using pisa on them? JPK From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Atul Kumar Sent: Monday, May 04, 2015 1:56 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] calculation of ASA between two helices Hi all,

[ccp4bb] cryo condition

Hello everyone Can anybody suggest me a cryo condition for a crystal obtained in MIDAS screen of Molecular Dimension: G1 0.1MTris8.0G10.1Mpotassium chloride25% v/vSOKALAN®CP7 0.1MHEPES7.0 G20.3Mammonium formate20% v/vSOKALAN® CP 5 0.1MHEPES7.0 Crystals are in beautiful cuboid shaped but all

Re: [ccp4bb] cryo condition

How long are you cryoprotecting for? You don't really need more than a few seconds. If I have sensitive crystals i tend to just swipe them slowly through the final CP solution and often that works. If I would leave them in the CP solution for more than say 30 seconds the crystals would behave

Re: [ccp4bb] cryo condition

High concentration of ammonium formate is a cryosolvent On Mon, May 4, 2015 at 5:20 PM, Tristan Croll tristan.cr...@qut.edu.au wrote: What about nature's favourite cryoprotectant, trehalose? From: CCP4 bulletin board CCP4BB@JISCMAIL.AC.UK on behalf of

Re: [ccp4bb] cryo condition

What about nature's favourite cryoprotectant, trehalose? From: CCP4 bulletin board CCP4BB@JISCMAIL.AC.UK on behalf of Roger Rowlett rrowl...@colgate.edu Sent: Tuesday, 5 May 2015 7:22 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] cryo condition We

[ccp4bb] Question for those who use AutoSharp via CCP4i on MacOSX Yosemite

Dear All, when starting CCP4i via the icon, AutoSharp remains greyed out, suggesting it is not installed. However, when I start CCP4i in an XQuartz window (i.e. “ccp4i ”), AutoSharp can be run. I guess this may be because in first way my “.profile” file is not read, any ideas how to fix this?

Re: [ccp4bb] cryo condition

We rarely use glycerol anymore, because it seems to fail so often for many of our current proteins. Try glucose, 25-30%. This is most conveniently done by weighing 125-150 mg of glucose in a microcentrifuge tube, then addding well solution to the 0.5 mL mark and mixing until completely

Re: [ccp4bb] cryo condition

If in doubt, try dragging the crystal through a 1:1 mix of Paratone-N and Mineral oil until most or all the mother liquor from surrounding the crystal has been removed. There are more tips here: http://hamptonresearch.com/tip_detail.aspx?id=99 Good luck! D On Mon, 4 May 2015 19:45 Faisal