Re: [ccp4bb] Nitrate versus Carbonate

Well, I was looking at two periplasmic binding proteins, one for NO3 and one for CO3/HCO3, and was wondering whether the obligate cooperative Ca ion adjacent to the ligand only in the CO3 binder was necessary, and how in any case it would help distinguish the two if at all. Roger’s comments abou

Re: [ccp4bb] Nitrate versus Carbonate

Dear JPK What I know is we do not have much nitrate in the blood unlike carbonate/bicarbonate. Nitrite, a nitrate byproduct can cause problems but it has a different structure and probably does not interfere with bicarbonate binding to relevant proteins. Cheers, Clement From: Keller, Jacob S

Re: [ccp4bb] Nitrate versus Carbonate

Bicarbonate ion is a weak base and is normally protonated near neutral pH. (Nitrate is a pathetically weak base and is not protonated at any reasonable biological pH.) Therefore, bicarbonate will have one hydrogen bond donor group (the -OH group) and two hydrogen bond acceptor groups, whereas nitra

Re: [ccp4bb] Nitrate versus Carbonate

Dear JPK, to answer your first question, at atomic resolution you would notice a density difference between N and C. At a little less resolution you might still measure difference in bond length. Regrds, Tim On Thursday, November 10, 2016 8:41:43 PM CET Keller, Jacob wrote: > Dear Crystallog

Re: [ccp4bb] Nitrate versus Carbonate

Would this figure answer your question? Cells must have way of feeling the difference in the bond lengths, and strength and nuances in hybridization. At least, I hope they do. Regards, Vaheh Oganesyan www.medimmune.com From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Kelle

Re: [ccp4bb] Post doctoral position in small molecule crystallography

Hello Krishnayan, If you are interested in Europe in the webpage of Euraxess write chemical crystallography. These days there is this position: http://ec.europa.eu/euraxess/index.cfm/jobs/jobDetails/34119496 Best wishes. Rosario On Thu, Nov 10, 2016 at 10:47 PM, Krishnayan Basuroy wrote: > De

Re: [ccp4bb] Nitrate versus Carbonate

Ignore my post.. should be a proton on one of the oxygens in carbonate. Interesting question! From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Jordan Baumhardt [jordan.baumha...@utsouthwestern.edu] Sent: Thursday, November 10, 2016 2:57 PM To: CCP4BB

Re: [ccp4bb] Post doctoral position in small molecule crystallography

This is the jobs site for the American Crystallographic Ass'n. http://www.amercrystalassn.org/content/pages/main-job-board None of these is obviously small-molecule (I thought it was called "high-resolution.") Anyway it's the only place I can think of. Good luck, Bob On Thu, 10 Nov 2016, K

[ccp4bb] Post doctoral position in small molecule crystallography

Dear Friends, Is there any website or bulletin board where people can find postdoctoral position in the field of small molecule crystallography? Best Regards, Krishnayan Basuroy

[ccp4bb] Nitrate versus Carbonate

Dear Crystallographers, I don't think there is any feasible way crystallographically to distinguish between nitrate and carbonate or bicarbonate-correct? But that is not my main question. My main question is: given that nitrate and carbonate are both very important and also very different phys

[ccp4bb] Fixing ligand B factors or refining group B shift?

Hello All OK back from political to scientific discussions (way more fun)! I've trawled through the Refmac documentation and not found a way of either fixing the Biso's of a ligand (or ligands) while still refining the Bisos for the protein, or refining just the group Biso shift(s) for the ligand

Re: [ccp4bb] FW: [ccp4bb] Fwd: [ccp4bb] just out of totally idle curiosity ...

From the Washington Post (https://www.washingtonpost.com/news/speaking-of-science/wp/2016/11/09/what-will-president-trump-mean-for-science/): Last year, Trumptold conservative radio host Michael Savage, “I hear

Re: [ccp4bb] Trump

Exactly, keep political opinions out of this discussion board. You have enough social media outlets to vent your frustrations. Dr. Chaithanya Madhurantakam Ramalingaswamy fellow (DBT), HOD, Professor (Asst.), Department of Biotechnology, TERI university, New Delhi www.madhurantakam.com On 10-Nov

Re: [ccp4bb] Trump

I had the impression that we are here to discuss science. If you want to discuss politics facebook is better place for you. On 9 November 2016 at 22:34, Charu Gupta wrote: > It is deeply saddening that a global super power's people would choose > such a leader. One can only hope now that he come

[ccp4bb] Postdoctoral Position in the Liang laboratory at Emory University (Cyo-EM Focused)

Dear colleagues, Applications are invited for a postdoctoral position in the Liang laboratory at Emory University School of Medicine. The principal goal of the Liang laboratory is to scrutinize high-resolution structural details and to understand the molecular mechanisms of large assemblies usi

[ccp4bb] Eppendorf epMotion 5070

Hi, Our lab is looking into buying a liquid handler system. Currently we are looking into Eppendorf epMotion 5070. Has any one had the chance to work with this system and if so, what is their opinion about the instrument? Furthermore, are there liquid handlers that members of the CCP4BB are partic

Re: [ccp4bb] [ccp4bb] Fwd: [ccp4bb] just out of totally idle curiosity ...

Sorry - didn't realise that was going out to the whole list, and just made a liar out of myself. Will bow out now. On 2016-11-10 10:20, Tristan Croll wrote: Hi Elton, I certainly didn't say I agreed with their choice or thought it was a good one. But as someone who grew up in a low-income, low

Re: [ccp4bb] [ccp4bb] Fwd: [ccp4bb] just out of totally idle curiosity ...

Hi Elton, I certainly didn't say I agreed with their choice or thought it was a good one. But as someone who grew up in a low-income, low-employment environment myself this has the ring of truth to it. I totally agree with you (and worry just as much) regarding the proliferation of anti-exper

Re: [ccp4bb] just out of totally idle curiosity ...

A good start would be to banish online social media and only use old fashioned newspapers as an outlet for any discourse in society. That includes banning reader unvetted comments sections on online newspapers. All those "follow us on facebook/twitter" links on webpages may seem nifty and cute

Re: [ccp4bb] [ccp4bb] Fwd: [ccp4bb] just out of totally idle curiosity ...

Hi Tristan, I’m afraid I don’t get the logic in the article you sent. In this case the farmers who are crying for the attention of the people in shiny palaces have voted for one who lives in golden mansions. For me the really worrying thing here is how can so many people be misled by a false

Re: [ccp4bb] [ccp4bb] Fwd: [ccp4bb] just out of totally idle curiosity ...

From letters in yesterday’s Guardian:- today is 9/11 Make America grate again > On 10 Nov 2016, at 08:00, Marjolein Thunnissen > wrote: > > Dear Bill, > > I fully agree with you, awareness has to be spread and one should not ignore > politics completely, especially when there are so stron

Re: [ccp4bb] [ccp4bb] Fwd: [ccp4bb] just out of totally idle curiosity ...

In the interests of promoting understanding... the link below is to an article on what is ostensibly a comedy website and contains a bit of coarse language, but nevertheless is quite possibly the most insightful exposition of the situation I've come across. The two-sentence synopsis: don't thin

Re: [ccp4bb] [ccp4bb] Fwd: [ccp4bb] just out of totally idle curiosity ...

Dear Bill, I fully agree with you, awareness has to be spread and one should not ignore politics completely, especially when there are so strong anti-intellectual (anti-science) statements out there. best regards Marjolein On 10 Nov 2016, at 04:17, William G. Scott mailto:wgsc...@ucsc.edu>>