Hi Mohinder,
if you are up to trying something new the monolithic columns by Biaseparation
(analytical or prep) are well suited for big molecules / particles and worked
very well for me. The fittings work with the GE chromatography instruments.
https://www.biaseparations.com
Good luc
Mohinder,
You could also try HPLC where you should get better results due to more
theoretical plates. One option would from Phenomenex.
10E6A Phase Information
--
- Molecular Weight Range: 60 K - 10,000 K
https://www.phenomenex.com/Products/HPLCDetail/phenogel
*D
How about BioGel-A50m with an exclusion limit of 50 million Da? I've used
A15m for complexes as large as 500 kDa and it seemed to work well.
__
Roger Rowlett
On Wed, Apr 17, 2019, 11:11 AM Mohinder Pal
wrote:
> Dear all,
>
> I would like to gel filter a multi protein complex (1.
Hi Mohinder,
Have you tried SEC columns like Sephacryl S-400 or CL-4B?
They have a higher fractionation range than Superose 6
HTH,
Dave
From: CCP4 bulletin board on behalf of Mohinder Pal
Sent: 17 April 2019 16:10
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4b
I can comfirm, it is my favourite feature of i2.
I would like to see it automated (or -able using CLI) for flawless
backup recovery.
Cheers,
Jan
On 16/04/2019 14:22, Christian Roth wrote:
> yes one can
>
> Cheers
> Christian
>
> On Tue, Apr 16, 2019 at 1:19 PM Eleanor Dodson
> <176a9d5ebad7
Dear all,
I would like to gel filter a multi protein complex (1.4MDa) as the final
purification step. I have tried tandem Superose 6 columns and this complex
elutes close to void volume as it is a very elongated molecule.
I was wondering if someone could suggest different analytical columns fo
Hi Phoebe,
that actually worked! thank you very much!
Best,
Almudena
El mar., 16 abr. 2019 a las 20:32, Phoebe A. Rice ()
escribió:
> At least last fall, the state of the art for getting your T7-transcribed
> RNA to start with a triphosphate in phenix was to add this file (edited for
> your ch
Hi,
No-one else seems to have said this, but just from the data in that table, if I
were a referee I would almost certainly be asking why you seem to have
discarded useful data at higher resolution than 3.1A!
Best wishes,
Randy Read
> On 16 Apr 2019, at 20:59, Jan van Agthoven wrote:
>
> De
Send your manuscripts to any of the IUCr journals!
Might still get the occasional referee like that, but the editors should ignore
those comments.
plus you'd be supporting a scientific society [sorry for the advert...]
Mark J van Raaij
Dpto de Estructura de Macromoleculas
Centro Nacional de Biot
