No love for RNA!? Ouch !
I was in the similar problem ten years ago when I was solving riboswitch RNAs
as a graduate student. B12 riboswitch,stuck at 5-6A for derivatives, but had
pretty reasonable native data.
It was a combination of experimental phasing using clusters at low resolution
and
Dear Charlie, Eleanor et al:
"Hate" might be a wee bit extreme, but I fully appreciate that RNA certainly
can be rather frustrating.
(I used to say the only things that catalytic RNA degraded faster than itself
were my self-esteem and career prospects.)
I managed to crystallize the same RNA m
How I hate RNA!
However, structures have been solved..
As others say:
Look for twinning
Look for translation non-cryst symmetry.
It seems likely when you have a doubling of the a axis for the Zn
derivative.
I use CCP4I2 and the data processing report will do both these tests.
Then you need to dec
Dear Charlie Nichols,
when optimizing your SHELX runs, SHELIXIR may help you in some cases
(https://kmlinux.fjfi.cvut.cz/~kolenpe1/shelixir).
A couple of days ago, I created quick and dirty tutorial to SHELIXIR command
line: https://www.youtube.com/watch?v=dqi5_yLhWOc and also the same quality
t
Hi Charles,
Congratulations on getting an RNA heteroduplex to crystallize! Most do not
crystallize.
You should make and look at a native Patterson map to find the off-origin
peak(s) that give the report of tNCS.
The direction and distance of this peak from the origin can be enlightening.
For an
Dear all,
I am trying to solve the structure of an RNA heteroduplex + ligand with
approximate MW of 6800.
* Structure likely to have a core helical region and a couple of bases of
single stranded material at both ends on both strands
I have datasets from visually similar crystals with diff
