Re: [ccp4bb] summary: crystals from skin

2009-01-14 Thread Jesse
stays on top. If the skin is required for crystal growth, then of course this doesn't apply. Generally, this is also simpler than converting the growth conditions from VD to batch under oil. -Jesse On Tue, Dec 30, 2008 at 11:22 AM, riya doreen driy...@gmail.com wrote: Hello Everyone, Thank you

Re: [ccp4bb] No diffraction

2012-02-07 Thread Jesse
This might be obvious, but make sure you washed the crystals thoroughly before dissolving them for SDS-PAGE or mass spec -Jesse On Thu, Jan 26, 2012 at 10:48 AM, Katherine Sippel katherine.sip...@gmail.com wrote: Might I suggest consulting the CCP4 user community wiki on the topic: http

Re: [ccp4bb] any review on protein-protein complex crystallization?

2008-05-14 Thread Jesse Sundlov
This may be obvious, but I would run a size-exclusion column on the complex first. This will give you an idea as to how well these two proteins stick together. On Wed, May 14, 2008 at 4:34 AM, Maarten Dewilde [EMAIL PROTECTED] wrote: Dear CCP4bb, Can anyone point me to good reviews or

[ccp4bb] Problem running BALBES

2009-01-28 Thread Jesse Sundlov
, in structDB_info_analysis rootElement = StripXml(struct_xml_document.documentElement) UnboundLocalError: local variable 'struct_xml_document' referenced before assignment Ideas? Any help would be greatly appreciated -Jesse

Re: [ccp4bb] Problem running BALBES

2009-01-29 Thread Jesse Sundlov
this newly found solution. It doesn't find a solution when using just the known structure of Protein 2 as a model. Is there a way to accomplish this? Sincerely, Jesse On Wed, Jan 28, 2009 at 1:25 PM, Dr. F Long f...@ysbl.york.ac.uk wrote: Dear Jesse, In both cases, there are some log

[ccp4bb] IMCA-CAT Announcement

2020-04-14 Thread Jesse Yoder
Dear Colleagues, The IMCA-CAT beamline at the Advanced Photon Source (APS) is continuing to operate and collect crystallography data during the current restricted-access operations at Argonne National Laboratory. Experiments are limited to (1) critical research related to COVID-19 or (2)

[ccp4bb] IMCA-CAT beam time is available for proprietary experiments

2020-05-06 Thread Jesse Yoder
proprietary agreements have been implemented for new industrial users to begin conducting experiments without delay. For more information about accessing IMCA-CAT, please contact Dr. Lisa Keefe (email is keefe [at] imca-cat.org). Work safely and stay healthy, Jesse Yoder -- Jesse Yoder, PhD