[ccp4bb] Assistant Professor Openings - U of Oklahoma

should be submitted as soon as possible, application review will begin on September 1, 2013. This position will remain open until filled. The University of Oklahoma is an equal opportunity institution. www.ou.edu/eoo Leonard Thomas, Ph.D. Macromolecular Crystallography Laboratory

[ccp4bb] content calculation in phaser

. The pdb file only has coordinates in it. Anybody else run into this problem? We were able to get a good solution using MolRep and it turns out to be 2 molecules in the symmetric unit in R32:H. If you input R32:H into Phaser it blows up. Regards, Len Leonard Thomas Ph.D. Macromolecular

Re: [ccp4bb] content calculation in phaser

in the asymmetric unit vs. one for the crystal that they were now working with. Lesson learned make sure your sequence files have the proper number of copies that you expect. Cheers, Len Leonard Thomas Ph.D. Macromolecular Crystallography Laboratory Manager University of Oklahoma Department

[ccp4bb] Protein Production Core Director - Job Opening - U or Oklahoma

is available immediately and will remain open until filled. For further information, please contact Dr. Ann West by phone (405) 325-1529 or email aw...@ou.edu. The University of Oklahoma is an equal opportunity institution. www.ou.edu/eoo Leonard Thomas, Ph.D. Macromolecular Crystallography

Re: [ccp4bb] bigger size - better diffraction?

The first thing to try before going down the long road of fussing with cryo is to take a shot at room temp. and see how your crystal diffracts in general. It is true it may be a cryo problem, but if the non cryo protected crystals do not diffract then why would one expect the cryo

Re: [ccp4bb] Popularity of Stereo graphics in crystallography

Hello, Personally I don't really care if I have a stereo setup or not. I have not really used stereo to build in probably 3-4 years. The majority of the users of my facility on the other hand really want stereo. Usually in the initial and final stages of building and analysis. It

[ccp4bb] shelx HKLF4 to mtz conversion?

I have a Shelx HKLF4 formated reflection file that I would like to convert to a mtz file. The primary reason is to transfer the freeR set. I found a couple of convoluted ways of converting the reflection data, but no real way of getting the freeR set to transfer. Any suggestions? Cheers

[ccp4bb] metal atoms written from coot in refmac

I have run across a problem and I am not sure where it originates from. When I use a pdb input file that contains a metal atom, say Cu, that has been written out by coot in refmac I get a read error of the sort that refmac does not recognize the Cu. If I then edit the pdb file and change

Re: [ccp4bb] O/T: can a protein which dimerizes in solution crystallize as a monomer?

puzzling over it and have tried a number of things to see if the structure is biologically significant. Leonard Thomas Ph. D. Macromolecular Crystallography Laboratory Manager University of Oklahoma Department of Chemistry and Biochemistry 620 Parrington Oval Norman, OK 73032 lmtho...@ou.edu

Re: [ccp4bb] Crystallography plates, hanging drop but templated sealing film.

Why not just use a 96 well plate. Should be able to do this by hand with a large enough drop. You can get them from either TTP Labtech or Grace Biosystems, I think that is the name. Cheers, Len Leonard Thomas Ph. D. Macromolecular Crystallography Laboratory Manager University of Oklahoma

Re: [ccp4bb] Off topic: Crystal degredation with age

. Of course these are probably more the exception then the rule. Len Leonard Thomas Ph. D. Macromolecular Crystallography Laboratory Manager University of Oklahoma Department of Chemistry and Biochemistry 620 Parrington Oval Norman, OK 73032 lmtho...@ou.edu Office: 405-325-1126 Lab: 405-325

[ccp4bb] problem with cns compile

+0x11ca): In function `vopen_': : undefined reference to `rename_' collect2: ld returned 1 exit status problems with new executable - old version retained This is a fresh install on this machine. Any ideas would be appreciated. Thanks, Leonard Thomas Ph. D. Macromolecular Crystallography Laboratory

Re: [ccp4bb] problem with cns compile - solution

Just an update, I ended up downloading binaries per Axel, and everything appears to be happy. Leonard Thomas Ph. D. Macromolecular Crystallography Laboratory Manager University of Oklahoma Department of Chemistry and Biochemistry 620 Parrington Oval Norman, OK 73019 lmtho...@ou.edu http

[ccp4bb] cryo protection

is that the crystals crack as soon as they are transfered out of the original drop. I am running out of ideas and really would love some new ones. Thanks in advance. Len Leonard Thomas Ph.D. Macromolecular Crystallography Laboratory Manager University of Oklahoma Department of Chemistry and Biochemistry

Re: [ccp4bb] cryo protection

when going into a separately made well solution. I will look it trying a number of suggestions given. Cheers, Len Leonard Thomas Ph.D. Macromolecular Crystallography Laboratory Manager University of Oklahoma Department of Chemistry and Biochemistry Stephenson Life Sciences Research Center 101

Re: [ccp4bb] issues with TLS refinement

. Leonard Thomas Ph.D. Macromolecular Crystallography Laboratory Manager University of Oklahoma Department of Chemistry and Biochemistry 620 Parrington Oval Norman, OK 73019 lmtho...@ou.edu http://barlywine.chem.ou.edu Office: (405)325-1126 Lab: (405)325-7571 On May 20, 2009, at 12:32 PM, Seema Mittal

[ccp4bb] execute problems with ccp4i

seems to be ok. I have some users who are just learning and I really don't think they get much out of the learning process watching me on my personal computer run through things. Cheers, Len Leonard Thomas Ph.D. Macromolecular Crystallography Laboratory Manager University of Oklahoma

Re: [ccp4bb] execute problems with ccp4i

The scratch directory was there but it was owned by root for some reason. After deleting it and rerunning the set up scripts all works fine. Cheers, Leonard Thomas Ph.D. Macromolecular Crystallography Laboratory Manager University of Oklahoma Department of Chemistry and Biochemistry 620

Re: [ccp4bb] cracking crystal

the glycerol concentration very gradually. I had a colleague who said the protein he worked on in grad school had to be stepped up 1% increments or it would crack and fall apart. IIRC he had better then 2.0 ang. data for the final structure. Leonard Thomas Ph.D. Macromolecular

Re: [ccp4bb] Distinguishing between P6(5)22 and P6(1)22

Given some of the news reports about the testing of TSA in the US I would say you might have a good chance of getting the bomb on the plane. Now if you want more then 3 oz. of mouthwash you could be in trouble. Leonard Thomas lmtho...@ou.edu On Sep 9, 2009, at 6:18 PM, Ezra Peisach

Re: [ccp4bb] Questions about cryoprotectant

I have had a fair amount of success using 2.1-2.3 M Na Malonate as a cryo protectant for crystals from high salt conditions like yours. Fluorinated oils are also an option, they generally are a little easier to work with then Paratone. Good Luck Leonard Thomas Ph.D. Macromolecular