Re: [ccp4bb] naturally obsessed - the movie

[Chris Ulens]

Dear members of the ccp4bb,
I have a somewhat unusual question. For many years I have shown the movie 
“Naturally Obsessed - the making of a scientist” for educative purposes in my 
classes at the faculty of medicine in KU Leuven. I just noticed that the movie 
is no longer freely downloadable. 
https://pbsinternational.org/programs/naturally-obsessed/ PBS responded to me 
that they no longer have the distribution rights. Could anyone point me toward 
a downloadable movie or perhaps a DVD so I can show it in my class this 
semester?

Thank you very much in advance.
Chris

———
Prof. Chris Ulens
Laboratory of Structural Neurobiology
Department of Cellular and Molecular Medicine
Facuty of Medicine
Herestraat 49, PB901
3000 Leuven
Belgium



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[ccp4bb] PhD position structural biology of ligand-gated ion channels

[Chris Ulens]

Dear members of the community,
A PhD position is available in the Laboratory of Prof. Chris Ulens, KU Leuven, 
Belgium (http://www.xtal.be<http://www.xtal.be/>). The research in the 
Laboratory of Structural Neurobiology is aimed at understanding the molecular 
mechanism of ligand-gated ion channels. Our methods rely on X-ray 
crystallography and cryo-EM in combination with electrophysiological recordings 
and fluorescence-based techniques. We have access to an integrated 
macromolecular crystallization facility with a Mosquito crystallization robot 
and two RockImagers. We have access to a 200 kV Glacios microscope on campus as 
well as 300 kV JEOL CryoARM300 at the nearby EM facility in Brussels. Current 
projects are situated at the crossroads of structural elucidation and drug 
discovery of different pentameric ligand-gated ion channels.

We would like to recruit a PhD student with prior experience in purification, 
cryo-EM or X-ray crystallography of membrane proteins. The position is open 
immediately and applications will be accepted until the position is filled. 
Applicants are requested to submit a CV with publication list and two 
references. E-mail to chris.ul...@kuleuven.be<mailto:chris.ul...@kuleuven.be>


Selection of recent publications:


The molecular mechanism of snake short-chain α-neurotoxin binding to 
muscle-type nicotinic acetylcholine receptors. Nys M, Zarkadas E, Brams M, 
Mehregan A, Kambara K, Kool J, Casewell NR, Bertrand D, Baenziger JE, Nury H, 
Ulens C. Nat Commun. 2022 Aug 4;13(1):4543.

The structural basis for an on-off switch controlling Gβγ-mediated inhibition 
of TRPM3 channels. Behrendt M, Gruss F, Enzeroth R, Dembla S, Zhao S, Crassous 
PA, Mohr F, Nys M, Louros N, Gallardo R, Zorzini V, Wagner D, Economou A, 
Rousseau F, Schymkowitz J, Philipp SE, Rohacs T, Ulens C, Oberwinkler J. Proc 
Natl Acad Sci U S A. 2020;117(46):29090-29100. doi: 10.1073/pnas.2001177117.

Modulation of the Erwinia ligand-gated ion channel (ELIC) and the 5-HT3 
receptor via a common vestibule site. Brams M, Govaerts C, Kambara K, Price KL, 
Spurny R, Gharpure A, Pardon E, Evans GL, Bertrand D, Lummis SC, Hibbs RE, 
Steyaert J, Ulens C. Elife. 2020;9:e51511. doi: 10.7554/eLife.51511.

A lipid site shapes the agonist response of a pentameric ligand-gated ion 
channel. Hénault CM, Govaerts C, Spurny R, Brams M, Estrada-Mondragon A, Lynch 
J, Bertrand D, Pardon E, Evans GL, Woods K, Elberson BW, Cuello LG, Brannigan 
G, Nury H, Steyaert J, Baenziger JE, Ulens C. Nat Chem Biol. 
2019;15(12):1156-1164. doi: 10.1038/s41589-019-0369-4.



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https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1

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Re: [ccp4bb] PhD position structural biology of ligand-gated ion channels

[Chris Ulens]

Dear members of the community,
A PhD position is available in the Laboratory of Prof. Chris Ulens, KU Leuven, 
Belgium (http://www.xtal.be). The research in the Laboratory of Structural 
Neurobiology is aimed at understanding the molecular mechanism of ligand-gated 
ion channels. Our methods rely on X-ray crystallography and cryo-EM in 
combination with electrophysiological recordings and fluorescence-based 
techniques. We have access to an integrated macromolecular crystallization 
facility with a Mosquito crystallization robot and two RockImagers. We have 
access to a 200 kV Glacios microscope on campus as well as 300 kV JEOL 
CryoARM300 at the nearby EM facility in Brussels. Current projects are situated 
at the crossroads of structural elucidation and drug discovery of different 
pentameric ligand-gated ion channels.

We would like to recruit a PhD student with prior experience in purification, 
cryo-EM or X-ray crystallography of membrane proteins. The position is open 
immediately and applications will be accepted until the position is filled. 
Applicants are requested to submit a CV with publication list and two 
references. E-mail to chris.ul...@kuleuven.be


Selection of recent publications:

 
The molecular mechanism of snake short-chain α-neurotoxin binding to 
muscle-type nicotinic acetylcholine receptors. Nys M, Zarkadas E, Brams M, 
Mehregan A, Kambara K, Kool J, Casewell NR, Bertrand D, Baenziger JE, Nury H, 
Ulens C. Nat Commun. 2022 Aug 4;13(1):4543. 

The structural basis for an on-off switch controlling Gβγ-mediated inhibition 
of TRPM3 channels. Behrendt M, Gruss F, Enzeroth R, Dembla S, Zhao S, Crassous 
PA, Mohr F, Nys M, Louros N, Gallardo R, Zorzini V, Wagner D, Economou A, 
Rousseau F, Schymkowitz J, Philipp SE, Rohacs T, Ulens C, Oberwinkler J. Proc 
Natl Acad Sci U S A. 2020;117(46):29090-29100. doi: 10.1073/pnas.2001177117.

Modulation of the Erwinia ligand-gated ion channel (ELIC) and the 5-HT3 
receptor via a common vestibule site. Brams M, Govaerts C, Kambara K, Price KL, 
Spurny R, Gharpure A, Pardon E, Evans GL, Bertrand D, Lummis SC, Hibbs RE, 
Steyaert J, Ulens C. Elife. 2020;9:e51511. doi: 10.7554/eLife.51511.

A lipid site shapes the agonist response of a pentameric ligand-gated ion 
channel. Hénault CM, Govaerts C, Spurny R, Brams M, Estrada-Mondragon A, Lynch 
J, Bertrand D, Pardon E, Evans GL, Woods K, Elberson BW, Cuello LG, Brannigan 
G, Nury H, Steyaert J, Baenziger JE, Ulens C. Nat Chem Biol. 
2019;15(12):1156-1164. doi: 10.1038/s41589-019-0369-4.





To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1

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Re: [ccp4bb] PhD position available

[Chris Ulens]

A position for a PhD student is available at the Laboratory of Prof. Chris 
Ulens, KU Leuven, Belgium (http://www.xtal.be). The research in the Laboratory 
of Structural Neurobiology is aimed at understanding the molecular mechanism of 
ligand-gated ion channels. Our methods rely on X-ray crystallography and 
cryo-EM in combination with electrophysiological recordings to relate structure 
to function. Our laboratory hosts an integrated macromolecular crystallization 
facility with a Mosquito crystallization robot and two RockImagers. We have 
access to a 200 kV Glacios microscope on campus as well as 300 kV JEOL 
CryoARM300 at the nearby EM facility in Brussels. Current projects are situated 
at the crossroads of structural elucidation and drug discovery of different 
pentameric ligand-gated ion channels.

We would like to recruit a PhD student with some experience in cryo-EM, X-ray 
crystallography, or purification of integral membrane proteins.

The successful candidate:

- easily communicates in an open verbal manner
- has a team player spirit
- has an ambitious work ethic
- critically contributes to writing of manuscripts and grant proposals
- meets intermediate deadlines and brings projects to a successful end
- fuels internal discussions of recent publications in the field


The position is open immediately and applications will be accepted until the 
position is filled. Applicants are requested to submit a CV via e-mail to 
chris.ul...@kuleuven.be<mailto:chris.ul...@kuleuven.be>


Selection of recent publications:


A lipid site shapes the agonist response of a pentameric ligand-gated ion 
channel. Hénault CM, Govaerts C, Spurny R, Brams M, Estrada-Mondragon A, Lynch 
J, Bertrand D, Pardon E, Evans GL, Woods K, Elberson BW, Cuello LG, Brannigan 
G, Nury H, Steyaert J, Baenziger JE, Ulens C. Nat Chem Biol. 
2019;15(12):1156-1164. doi: 10.1038/s41589-019-0369-4.


Modulation of the Erwinia ligand-gated ion channel (ELIC) and the 5-HT3 
receptor via a common vestibule site. Brams M, Govaerts C, Kambara K, Price KL, 
Spurny R, Gharpure A, Pardon E, Evans GL, Bertrand D, Lummis SC, Hibbs RE, 
Steyaert J, Ulens C. Elife. 2020;9:e51511. doi: 10.7554/eLife.51511.


The structural basis for an on-off switch controlling Gβγ-mediated inhibition 
of TRPM3 channels. Behrendt M, Gruss F, Enzeroth R, Dembla S, Zhao S, Crassous 
PA, Mohr F, Nys M, Louros N, Gallardo R, Zorzini V, Wagner D, Economou A, 
Rousseau F, Schymkowitz J, Philipp SE, Rohacs T, Ulens C, Oberwinkler J. Proc 
Natl Acad Sci U S A. 2020;117(46):29090-29100. doi: 10.1073/pnas.2001177117.



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1

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[ccp4bb] post-doc and PhD position at KU Leuven, ligand-gated ion channels

[Chris Ulens]

This is a kind reminder that the positions of post-doc and PhD in our lab are 
still open for applicants.

Best regards
Chris


> On 18 Jan 2022, at 16:20, Chris Ulens  wrote:
> 
> Dear members of the community,
> One post-doctoral position and one PhD position are available in the 
> Laboratory of Prof. Chris Ulens, KU Leuven, Belgium (http://www.xtal.be 
> <http://www.xtal.be/>). The research in the Laboratory of Structural 
> Neurobiology is aimed at understanding the molecular mechanism of 
> ligand-gated ion channels. Our methods rely on X-ray crystallography and 
> cryo-EM in combination with electrophysiological recordings and 
> fluorescence-based techniques. Our laboratory hosts an integrated 
> macromolecular crystallization facility with a Mosquito crystallization robot 
> and two RockImagers. We have access to a 200 kV Glacios microscope on campus 
> as well as 300 kV JEOL CryoARM300 at the nearby EM facility in Brussels. 
> Current projects are situated at the crossroads of structural elucidation and 
> drug discovery of different pentameric ligand-gated ion channels.
> 
> We would like to recruit a post-doc and PhD student with prior experience in 
> purification, cryo-EM or X-ray crystallography of membrane proteins. The 
> position is open immediately and applications will be accepted until the 
> position is filled. Applicants are requested to submit a CV with publication 
> list and two references. E-mail to chris.ul...@kuleuven.be 
> <mailto:chris.ul...@kuleuven.be>
> 
> 
> Selection of recent publications:
> 
> 
> A lipid site shapes the agonist response of a pentameric ligand-gated ion 
> channel. Hénault CM, Govaerts C, Spurny R, Brams M, Estrada-Mondragon A, 
> Lynch J, Bertrand D, Pardon E, Evans GL, Woods K, Elberson BW, Cuello LG, 
> Brannigan G, Nury H, Steyaert J, Baenziger JE, Ulens C. Nat Chem Biol. 
> 2019;15(12):1156-1164. doi: 10.1038/s41589-019-0369-4.
> 
> 
> Modulation of the Erwinia ligand-gated ion channel (ELIC) and the 5-HT3 
> receptor via a common vestibule site. Brams M, Govaerts C, Kambara K, Price 
> KL, Spurny R, Gharpure A, Pardon E, Evans GL, Bertrand D, Lummis SC, Hibbs 
> RE, Steyaert J, Ulens C. Elife. 2020;9:e51511. doi: 10.7554/eLife.51511.
> 
> 
> The structural basis for an on-off switch controlling Gβγ-mediated inhibition 
> of TRPM3 channels. Behrendt M, Gruss F, Enzeroth R, Dembla S, Zhao S, 
> Crassous PA, Mohr F, Nys M, Louros N, Gallardo R, Zorzini V, Wagner D, 
> Economou A, Rousseau F, Schymkowitz J, Philipp SE, Rohacs T, Ulens C, 
> Oberwinkler J. Proc Natl Acad Sci U S A. 2020;117(46):29090-29100. doi: 
> 10.1073/pnas.2001177117.
> 
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 
> <https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1>



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Description: S/MIME cryptographic signature

Re: [ccp4bb] post-doc and PhD position at KU Leuven, ligand-gated ion channels

[Chris Ulens]

Dear members of the community,
One post-doctoral position and one PhD position are available in the Laboratory 
of Prof. Chris Ulens, KU Leuven, Belgium (http://www.xtal.be 
<http://www.xtal.be/>). The research in the Laboratory of Structural 
Neurobiology is aimed at understanding the molecular mechanism of ligand-gated 
ion channels. Our methods rely on X-ray crystallography and cryo-EM in 
combination with electrophysiological recordings and fluorescence-based 
techniques. Our laboratory hosts an integrated macromolecular crystallization 
facility with a Mosquito crystallization robot and two RockImagers. We have 
access to a 200 kV Glacios microscope on campus as well as 300 kV JEOL 
CryoARM300 at the nearby EM facility in Brussels. Current projects are situated 
at the crossroads of structural elucidation and drug discovery of different 
pentameric ligand-gated ion channels.

We would like to recruit a post-doc and PhD student with prior experience in 
purification, cryo-EM or X-ray crystallography of membrane proteins. The 
position is open immediately and applications will be accepted until the 
position is filled. Applicants are requested to submit a CV with publication 
list and two references. E-mail to chris.ul...@kuleuven.be 
<mailto:chris.ul...@kuleuven.be>


Selection of recent publications:


A lipid site shapes the agonist response of a pentameric ligand-gated ion 
channel. Hénault CM, Govaerts C, Spurny R, Brams M, Estrada-Mondragon A, Lynch 
J, Bertrand D, Pardon E, Evans GL, Woods K, Elberson BW, Cuello LG, Brannigan 
G, Nury H, Steyaert J, Baenziger JE, Ulens C. Nat Chem Biol. 
2019;15(12):1156-1164. doi: 10.1038/s41589-019-0369-4.


Modulation of the Erwinia ligand-gated ion channel (ELIC) and the 5-HT3 
receptor via a common vestibule site. Brams M, Govaerts C, Kambara K, Price KL, 
Spurny R, Gharpure A, Pardon E, Evans GL, Bertrand D, Lummis SC, Hibbs RE, 
Steyaert J, Ulens C. Elife. 2020;9:e51511. doi: 10.7554/eLife.51511.


The structural basis for an on-off switch controlling Gβγ-mediated inhibition 
of TRPM3 channels. Behrendt M, Gruss F, Enzeroth R, Dembla S, Zhao S, Crassous 
PA, Mohr F, Nys M, Louros N, Gallardo R, Zorzini V, Wagner D, Economou A, 
Rousseau F, Schymkowitz J, Philipp SE, Rohacs T, Ulens C, Oberwinkler J. Proc 
Natl Acad Sci U S A. 2020;117(46):29090-29100. doi: 10.1073/pnas.2001177117.


To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1

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Re: [ccp4bb] post-doc and PhD position at KU Leuven, ligand-gated ion channels

[Chris Ulens]

One post-doctoral position and one PhD position are available in the Laboratory 
of Prof. Chris Ulens, KU Leuven, Belgium (http://www.xtal.be 
<http://www.xtal.be/>). The research in the Laboratory of Structural 
Neurobiology is aimed at understanding the molecular mechanism of ligand-gated 
ion channels. Our methods rely on X-ray crystallography and cryo-EM in 
combination with electrophysiological recordings and fluorescence-based 
techniques. Our laboratory hosts an integrated macromolecular crystallization 
facility with a Mosquito crystallization robot and two RockImagers. We have 
access to a 200 kV Glacios microscope on campus as well as 300 kV JEOL 
CryoARM300 at the nearby EM facility in Brussels. Current projects are situated 
at the crossroads of structural elucidation and drug discovery of different 
pentameric ligand-gated ion channels.

We would like to recruit a post-doc and PhD student with prior experience in 
purification, cryo-EM or X-ray crystallography of membrane proteins. The 
position is open immediately and applications will be accepted until the 
position is filled. Applicants are requested to submit a CV with publication 
list and two references. E-mail to chris.ul...@kuleuven.be 
<mailto:chris.ul...@kuleuven.be>


Selection of recent publications:


A lipid site shapes the agonist response of a pentameric ligand-gated ion 
channel. Hénault CM, Govaerts C, Spurny R, Brams M, Estrada-Mondragon A, Lynch 
J, Bertrand D, Pardon E, Evans GL, Woods K, Elberson BW, Cuello LG, Brannigan 
G, Nury H, Steyaert J, Baenziger JE, Ulens C. Nat Chem Biol. 
2019;15(12):1156-1164. doi: 10.1038/s41589-019-0369-4.


Modulation of the Erwinia ligand-gated ion channel (ELIC) and the 5-HT3 
receptor via a common vestibule site. Brams M, Govaerts C, Kambara K, Price KL, 
Spurny R, Gharpure A, Pardon E, Evans GL, Bertrand D, Lummis SC, Hibbs RE, 
Steyaert J, Ulens C. Elife. 2020;9:e51511. doi: 10.7554/eLife.51511.


The structural basis for an on-off switch controlling Gβγ-mediated inhibition 
of TRPM3 channels. Behrendt M, Gruss F, Enzeroth R, Dembla S, Zhao S, Crassous 
PA, Mohr F, Nys M, Louros N, Gallardo R, Zorzini V, Wagner D, Economou A, 
Rousseau F, Schymkowitz J, Philipp SE, Rohacs T, Ulens C, Oberwinkler J. Proc 
Natl Acad Sci U S A. 2020;117(46):29090-29100. doi: 10.1073/pnas.2001177117.


To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1

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Re: [ccp4bb] post-doc position at KU Leuven, ligand-gated ion channels

[Chris Ulens]

A post-doctoral position is available at the Laboratory of Prof. Chris Ulens, 
KU Leuven, Belgium (http://www.xtal.be <http://www.xtal.be/>). The research in 
the Laboratory of Structural Neurobiology is aimed at understanding the 
molecular mechanism of ligand-gated ion channels. Our methods rely on X-ray 
crystallography and cryo-EM in combination with electrophysiological recordings 
and fluorescence-based techniques. Our laboratory hosts an integrated 
macromolecular crystallization facility with a Mosquito crystallization robot 
and two RockImagers. We have access to a 200 kV Glacios microscope on campus as 
well as 300 kV JEOL CryoARM300 at the nearby EM facility in Brussels. Current 
projects are situated at the crossroads of structural elucidation and drug 
discovery of different pentameric ligand-gated ion channels.

We would like to recruit a post-doc with prior experience in cryo-EM or X-ray 
crystallography of membrane proteins. The position is open immediately and 
applications will be accepted until the position is filled. Applicants are 
requested to submit a CV with publication list and two references. E-mail to 
chris.ul...@kuleuven.be <mailto:chris.ul...@kuleuven.be>


Selection of recent publications:


A lipid site shapes the agonist response of a pentameric ligand-gated ion 
channel. Hénault CM, Govaerts C, Spurny R, Brams M, Estrada-Mondragon A, Lynch 
J, Bertrand D, Pardon E, Evans GL, Woods K, Elberson BW, Cuello LG, Brannigan 
G, Nury H, Steyaert J, Baenziger JE, Ulens C. Nat Chem Biol. 
2019;15(12):1156-1164. doi: 10.1038/s41589-019-0369-4.


Modulation of the Erwinia ligand-gated ion channel (ELIC) and the 5-HT3 
receptor via a common vestibule site. Brams M, Govaerts C, Kambara K, Price KL, 
Spurny R, Gharpure A, Pardon E, Evans GL, Bertrand D, Lummis SC, Hibbs RE, 
Steyaert J, Ulens C. Elife. 2020;9:e51511. doi: 10.7554/eLife.51511.


The structural basis for an on-off switch controlling Gβγ-mediated inhibition 
of TRPM3 channels. Behrendt M, Gruss F, Enzeroth R, Dembla S, Zhao S, Crassous 
PA, Mohr F, Nys M, Louros N, Gallardo R, Zorzini V, Wagner D, Economou A, 
Rousseau F, Schymkowitz J, Philipp SE, Rohacs T, Ulens C, Oberwinkler J. Proc 
Natl Acad Sci U S A. 2020;117(46):29090-29100. doi: 10.1073/pnas.2001177117.


To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1

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hosted by www.jiscmail.ac.uk, terms & conditions are available at 
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[ccp4bb] post-doc position at KU Leuven, ligand-gated ion channels

[Chris Ulens]

A post-doctoral position is available at the Laboratory of Prof. Chris Ulens, 
KU Leuven, Belgium (http://www.xtal.be <http://www.xtal.be/>). The research in 
the Laboratory of Structural Neurobiology is aimed at understanding the 
molecular mechanism of ligand-gated ion channels. Our methods rely on X-ray 
crystallography and cryo-EM in combination with electrophysiological recordings 
and fluorescence-based techniques. Our laboratory hosts an integrated 
macromolecular crystallization facility with a Mosquito crystallization robot 
and two RockImagers. We have access to a 200 kV Glacios microscope on campus as 
well as 300 kV JEOL CryoARM300 at the nearby EM facility in Brussels. Current 
projects are situated at the crossroads of structural elucidation and drug 
discovery of different pentameric ligand-gated ion channels.

We would like to recruit a post-doc with prior experience in cryo-EM or X-ray 
crystallography of membrane proteins.

The successful candidate:

- easily communicates in an open verbal manner and has a team player spirit
- has an ambitious work ethic
- critically contributes to writing of manuscripts and grant proposals
- makes efforts to obtain independent funding
- meets intermediate deadlines and brings projects to a successful end
- fuels internal discussions of recent publications in the field
- sets an example to younger PhD students in the lab
- identifies directions for future scientific research


The position is open immediately and applications will be accepted until the 
position is filled. Applicants are requested to submit a CV with publication 
list and two references. E-mail to chris.ul...@kuleuven.be 
<mailto:chris.ul...@kuleuven.be>


Selection of recent publications:


A lipid site shapes the agonist response of a pentameric ligand-gated ion 
channel. Hénault CM, Govaerts C, Spurny R, Brams M, Estrada-Mondragon A, Lynch 
J, Bertrand D, Pardon E, Evans GL, Woods K, Elberson BW, Cuello LG, Brannigan 
G, Nury H, Steyaert J, Baenziger JE, Ulens C. Nat Chem Biol. 
2019;15(12):1156-1164. doi: 10.1038/s41589-019-0369-4.


Modulation of the Erwinia ligand-gated ion channel (ELIC) and the 5-HT3 
receptor via a common vestibule site. Brams M, Govaerts C, Kambara K, Price KL, 
Spurny R, Gharpure A, Pardon E, Evans GL, Bertrand D, Lummis SC, Hibbs RE, 
Steyaert J, Ulens C. Elife. 2020;9:e51511. doi: 10.7554/eLife.51511.


The structural basis for an on-off switch controlling Gβγ-mediated inhibition 
of TRPM3 channels. Behrendt M, Gruss F, Enzeroth R, Dembla S, Zhao S, Crassous 
PA, Mohr F, Nys M, Louros N, Gallardo R, Zorzini V, Wagner D, Economou A, 
Rousseau F, Schymkowitz J, Philipp SE, Rohacs T, Ulens C, Oberwinkler J. Proc 
Natl Acad Sci U S A. 2020;117(46):29090-29100. doi: 10.1073/pnas.2001177117.


To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1

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hosted by www.jiscmail.ac.uk, terms & conditions are available at 
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[ccp4bb] post-doc position at KU Leuven, ligand-gated ion channels

[Chris Ulens]

A post-doctoral position is available at the Laboratory of Prof. Chris Ulens, 
KU Leuven, Belgium (http://www.xtal.be <http://www.xtal.be/>). The research in 
the Laboratory of Structural Neurobiology is aimed at understanding the 
molecular mechanism of ligand-gated ion channels. Our methods rely on X-ray 
crystallography and cryo-EM in combination with electrophysiological recordings 
and fluorescence-based techniques. Our laboratory hosts an integrated 
macromolecular crystallization facility with a Mosquito crystallization robot 
and two RockImagers. We have access to a 200 kV Glacios microscope on campus as 
well as 300 kV JEOL CryoARM300 at the nearby EM facility in Brussels. Current 
projects are situated at the crossroads of structural elucidation and drug 
discovery of different pentameric ligand-gated ion channels.

We would like to recruit a post-doc with prior experience in cryo-EM or X-ray 
crystallography of membrane proteins.

The successful candidate:

- easily communicates in an open verbal manner and has a team player spirit
- has an ambitious work ethic
- critically contributes to writing of manuscripts and grant proposals
- makes efforts to obtain independent funding
- meets intermediate deadlines and brings projects to a successful end
- fuels internal discussions of recent publications in the field
- sets an example to younger PhD students in the lab
- identifies directions for future scientific research


The position is open immediately and applications will be accepted until the 
position is filled. Applicants are requested to submit a CV with publication 
list and two references. E-mail to chris.ul...@kuleuven.be 
<mailto:chris.ul...@kuleuven.be>


Selection of recent publications:


A lipid site shapes the agonist response of a pentameric ligand-gated ion 
channel. Hénault CM, Govaerts C, Spurny R, Brams M, Estrada-Mondragon A, Lynch 
J, Bertrand D, Pardon E, Evans GL, Woods K, Elberson BW, Cuello LG, Brannigan 
G, Nury H, Steyaert J, Baenziger JE, Ulens C. Nat Chem Biol. 
2019;15(12):1156-1164. doi: 10.1038/s41589-019-0369-4.


Modulation of the Erwinia ligand-gated ion channel (ELIC) and the 5-HT3 
receptor via a common vestibule site. Brams M, Govaerts C, Kambara K, Price KL, 
Spurny R, Gharpure A, Pardon E, Evans GL, Bertrand D, Lummis SC, Hibbs RE, 
Steyaert J, Ulens C. Elife. 2020;9:e51511. doi: 10.7554/eLife.51511.


The structural basis for an on-off switch controlling Gβγ-mediated inhibition 
of TRPM3 channels. Behrendt M, Gruss F, Enzeroth R, Dembla S, Zhao S, Crassous 
PA, Mohr F, Nys M, Louros N, Gallardo R, Zorzini V, Wagner D, Economou A, 
Rousseau F, Schymkowitz J, Philipp SE, Rohacs T, Ulens C, Oberwinkler J. Proc 
Natl Acad Sci U S A. 2020;117(46):29090-29100. doi: 10.1073/pnas.2001177117.


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Re: [ccp4bb] post-doc position at KU Leuven, ligand-gated ion channels

[Chris Ulens]

A post-doctoral position is available at the Laboratory of Prof. Chris Ulens, 
KU Leuven, Belgium (http://www.xtal.be). The research in the Laboratory of 
Structural Neurobiology is aimed at understanding the molecular mechanism of 
ligand-gated ion channels. Our methods rely on X-ray crystallography and 
cryo-EM in combination with electrophysiological recordings and 
fluorescence-based techniques. Our laboratory hosts an integrated 
macromolecular crystallization facility with a Mosquito crystallization robot 
and two RockImagers. We have access to a 200 kV Glacios microscope on campus as 
well as 300 kV JEOL CryoARM300 at the nearby EM facility in Brussels. Current 
projects are situated at the crossroads of structural elucidation and drug 
discovery of different pentameric ligand-gated ion channels.

 We would like to recruit a post-doc with prior experience in cryo-EM or X-ray 
crystallography of membrane proteins.
 
The successful candidate:

- easily communicates in an open verbal manner and has a team player spirit
- has an ambitious work ethic
- critically contributes to writing of manuscripts and grant proposals
- makes efforts to obtain independent funding
- meets intermediate deadlines and brings projects to a successful end
- fuels internal discussions of recent publications in the field
- sets an example to younger PhD students in the lab
- identifies directions for future scientific research

 
The position is open immediately and applications will be accepted until the 
position is filled. Applicants are requested to submit a CV with publication 
list and two references. E-mail to chris.ul...@kuleuven.be

 
Selection of recent publications:

 
A lipid site shapes the agonist response of a pentameric ligand-gated ion 
channel. Hénault CM, Govaerts C, Spurny R, Brams M, Estrada-Mondragon A, Lynch 
J, Bertrand D, Pardon E, Evans GL, Woods K, Elberson BW, Cuello LG, Brannigan 
G, Nury H, Steyaert J, Baenziger JE, Ulens C. Nat Chem Biol. 
2019;15(12):1156-1164. doi: 10.1038/s41589-019-0369-4.

 
Modulation of the Erwinia ligand-gated ion channel (ELIC) and the 5-HT3 
receptor via a common vestibule site. Brams M, Govaerts C, Kambara K, Price KL, 
Spurny R, Gharpure A, Pardon E, Evans GL, Bertrand D, Lummis SC, Hibbs RE, 
Steyaert J, Ulens C. Elife. 2020;9:e51511. doi: 10.7554/eLife.51511.

 
The structural basis for an on-off switch controlling Gβγ-mediated inhibition 
of TRPM3 channels. Behrendt M, Gruss F, Enzeroth R, Dembla S, Zhao S, Crassous 
PA, Mohr F, Nys M, Louros N, Gallardo R, Zorzini V, Wagner D, Economou A, 
Rousseau F, Schymkowitz J, Philipp SE, Rohacs T, Ulens C, Oberwinkler J. Proc 
Natl Acad Sci U S A. 2020;117(46):29090-29100. doi: 10.1073/pnas.2001177117.


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Re: [ccp4bb] post-doc position at KU Leuven, ligand-gated ion channels

[Chris Ulens]

This is a reminder for interested candidates to apply for the vacant position 
below.

Thank you and best regards
Chris


> On 5 Jan 2021, at 17:33, Chris Ulens  wrote:
> 
> A post-doctoral position is available at the Laboratory of Prof. Chris Ulens, 
> KU Leuven, Belgium (http://www.xtal.be <http://www.xtal.be/>). The research 
> in the Laboratory of Structural Neurobiology is aimed at understanding the 
> molecular mechanism of ligand-gated ion channels. Our methods rely on X-ray 
> crystallography and cryo-EM in combination with electrophysiological 
> recordings and fluorescence-based techniques. Our laboratory hosts an 
> integrated macromolecular crystallization facility with a Mosquito 
> crystallization robot and two RockImagers. We have access to a 200 kV Glacios 
> microscope on campus as well as 300 kV JEOL CryoARM300 at the nearby EM 
> facility in Brussels. Current projects are situated at the crossroads of 
> structural elucidation and drug discovery of different pentameric 
> ligand-gated ion channels.
> 
>  
> We would like to recruit a post-doc with prior experience in cryo-EM or X-ray 
> crystallography of membrane proteins.
> 
>  
> The successful candidate:
> 
> - easily communicates in an open verbal manner and has a team player spirit
> 
> - has an ambitious work ethic
> 
> - critically contributes to writing of manuscripts and grant proposals
> 
> - makes efforts to obtain independent funding
> 
> - meets intermediate deadlines and brings projects to a successful end
> 
> - fuels internal discussions of recent publications in the field
> 
> - sets an example to younger PhD students in the lab
> 
> - identifies directions for future scientific research
> 
>  
> The position is open immediately and applications will be accepted until the 
> position is filled. Applicants are requested to submit a CV with publication 
> list and two references. E-mail to chris.ul...@kuleuven.be 
> <mailto:chris.ul...@kuleuven.be>
>  
> Selection of recent publications:
> 
>  
> A lipid site shapes the agonist response of a pentameric ligand-gated ion 
> channel. Hénault CM, Govaerts C, Spurny R, Brams M, Estrada-Mondragon A, 
> Lynch J, Bertrand D, Pardon E, Evans GL, Woods K, Elberson BW, Cuello LG, 
> Brannigan G, Nury H, Steyaert J, Baenziger JE, Ulens C. Nat Chem Biol. 
> 2019;15(12):1156-1164. doi: 10.1038/s41589-019-0369-4.
> 
>  
> Modulation of the Erwinia ligand-gated ion channel (ELIC) and the 5-HT3 
> receptor via a common vestibule site. Brams M, Govaerts C, Kambara K, Price 
> KL, Spurny R, Gharpure A, Pardon E, Evans GL, Bertrand D, Lummis SC, Hibbs 
> RE, Steyaert J, Ulens C. Elife. 2020;9:e51511. doi: 10.7554/eLife.51511.
> 
>  
> The structural basis for an on-off switch controlling Gβγ-mediated inhibition 
> of TRPM3 channels. Behrendt M, Gruss F, Enzeroth R, Dembla S, Zhao S, 
> Crassous PA, Mohr F, Nys M, Louros N, Gallardo R, Zorzini V, Wagner D, 
> Economou A, Rousseau F, Schymkowitz J, Philipp SE, Rohacs T, Ulens C, 
> Oberwinkler J. Proc Natl Acad Sci U S A. 2020;117(46):29090-29100. doi: 
> 10.1073/pnas.2001177117.
> 
> 
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 
> <https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1>



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Description: S/MIME cryptographic signature

[ccp4bb] post-doc position at KU Leuven, ligand-gated ion channels

[Chris Ulens]

A post-doctoral position is available at the Laboratory of Prof. Chris Ulens, 
KU Leuven, Belgium (http://www.xtal.be <http://www.xtal.be/>). The research in 
the Laboratory of Structural Neurobiology is aimed at understanding the 
molecular mechanism of ligand-gated ion channels. Our methods rely on X-ray 
crystallography and cryo-EM in combination with electrophysiological recordings 
and fluorescence-based techniques. Our laboratory hosts an integrated 
macromolecular crystallization facility with a Mosquito crystallization robot 
and two RockImagers. We have access to a 200 kV Glacios microscope on campus as 
well as 300 kV JEOL CryoARM300 at the nearby EM facility in Brussels. Current 
projects are situated at the crossroads of structural elucidation and drug 
discovery of different pentameric ligand-gated ion channels.

 

We would like to recruit a post-doc with prior experience in cryo-EM or X-ray 
crystallography of membrane proteins.

 

The successful candidate:

- easily communicates in an open verbal manner and has a team player spirit

- has an ambitious work ethic

- critically contributes to writing of manuscripts and grant proposals

- makes efforts to obtain independent funding

- meets intermediate deadlines and brings projects to a successful end

- fuels internal discussions of recent publications in the field

- sets an example to younger PhD students in the lab

- identifies directions for future scientific research

 

The position is open immediately and applications will be accepted until the 
position is filled. Applicants are requested to submit a CV with publication 
list and two references. E-mail to chris.ul...@kuleuven.be 
<mailto:chris.ul...@kuleuven.be>
 

Selection of recent publications:

 

A lipid site shapes the agonist response of a pentameric ligand-gated ion 
channel. Hénault CM, Govaerts C, Spurny R, Brams M, Estrada-Mondragon A, Lynch 
J, Bertrand D, Pardon E, Evans GL, Woods K, Elberson BW, Cuello LG, Brannigan 
G, Nury H, Steyaert J, Baenziger JE, Ulens C. Nat Chem Biol. 
2019;15(12):1156-1164. doi: 10.1038/s41589-019-0369-4.

 

Modulation of the Erwinia ligand-gated ion channel (ELIC) and the 5-HT3 
receptor via a common vestibule site. Brams M, Govaerts C, Kambara K, Price KL, 
Spurny R, Gharpure A, Pardon E, Evans GL, Bertrand D, Lummis SC, Hibbs RE, 
Steyaert J, Ulens C. Elife. 2020;9:e51511. doi: 10.7554/eLife.51511.

 

The structural basis for an on-off switch controlling Gβγ-mediated inhibition 
of TRPM3 channels. Behrendt M, Gruss F, Enzeroth R, Dembla S, Zhao S, Crassous 
PA, Mohr F, Nys M, Louros N, Gallardo R, Zorzini V, Wagner D, Economou A, 
Rousseau F, Schymkowitz J, Philipp SE, Rohacs T, Ulens C, Oberwinkler J. Proc 
Natl Acad Sci U S A. 2020;117(46):29090-29100. doi: 10.1073/pnas.2001177117.


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Re: [ccp4bb] post-doctoral position structural biology of ion channels

[chris . ulens]

A post-doctoral position is available at the Laboratory of Structural 
Neurobiology, KU Leuven Belgium (http://www.xtal.be ). The 
research in our laboratory is aimed at understanding the molecular mechanism of 
ligand-gated ion channels. Our method relies on X-ray crystallography and 
cryo-EM in combination with electrophysiological recordings and fluorescence 
spectroscopy. Our laboratory hosts an integrated macromolecular crystallization 
facility with a Mosquito crystallization robot and two RockImagers. Current 
structural targets include the family of pentameric ligand-gated ion channels 
(Cys-loop receptors), TRP channels and CNG channels.

We would like to recruit a post-doc with prior experience in expression, 
purification and crystallization of membrane proteins. Prior experience with 
X-ray crystallography or cryo-EM is considered a major advantage.

The successful candidate:
- easily communicates in an open verbal manner and has a team player spirit
- has an ambitious work ethic
- critically contributes to writing of manuscripts and grant proposals
- makes efforts to obtain independent funding
- meets intermediate deadlines and brings projects to a successful end
- fuels internal discussions of recent publications in the field
- sets an example to younger PhD students in the lab
- identifies directions for future scientific research

The position is open immediately and applications will be accepted until the 
position is filled. Applicants are requested to submit a CV with publication 
list and two references. E-mail to chris.ul...@kuleuven.be

Selected lab publications:

Molecular blueprint of allosteric binding sites in a homologue of the 
agonist-binding domain of the α7 nicotinic acetylcholine receptor. Spurny R, 
Debaveye S, Farinha A, Veys K, Vos AM, Gossas T, Atack J, Bertrand S, Bertrand 
D, Danielson UH, Tresadern G, Ulens C.
Proc Natl Acad Sci U S A. 2015 May 12;112(19):E2543-52.

Pentameric ligand-gated ion channel ELIC is activated by GABA and modulated by 
benzodiazepines. Spurny R, Ramerstorfer J, Price K, Brams M, Ernst M, Nury H, 
Verheij M, Legrand P, Bertrand D, Bertrand S, Dougherty DA, de Esch IJ, 
Corringer PJ, Sieghart W, Lummis SC, Ulens C. Proc Natl Acad Sci U S A. 2012 
Oct 30;109(44):E3028-34.

A structural and mutagenic blueprint for molecular recognition of strychnine 
and d-tubocurarine by different cys-loop receptors. Brams M, Pandya A, Kuzmin 
D, van Elk R, Krijnen L, Yakel JL, Tsetlin V, Smit AB, Ulens C.
PLoS Biol. 2011 Mar;9(3):e1001034.


To unsubscribe from the CCP4BB list, click the following link:
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[ccp4bb] post-doctoral position and PhD position structural biology of ion channels

[Chris Ulens]

A post-doctoral position and PhD position are available at the laboratory of 
Structural Neurobiology, KU Leuven Belgium. The lab website is available at 
http://www.xtal.be . The research in our laboratory is 
aimed at understanding the molecular mechanism of ligand-gated ion channels. 
Our method relies on X-ray crystallography and cryo-EM in combination with 
electrophysiological recordings and fluorescence spectroscopy. Our laboratory 
hosts an integrated macromolecular crystallization facility with a Mosquito 
crystallization robot and two RockImagers. Current structural targets include 
the family of pentameric ligand-gated ion channels (Cys-loop receptors), TRP 
channels and CNG channels.

We would like to recruit a post-doc and/or PhD student with prior experience in 
expression, purification and crystallization of membrane proteins. Prior 
experience with X-ray crystallography or cryo-EM is considered a major 
advantage.

The successful candidate:
- easily communicates in an open verbal manner and has a team player spirit
- has an ambitious work ethic
- critically contributes to writing of manuscripts and grant proposals
- makes efforts to obtain independent funding
- meets intermediate deadlines and brings projects to a successful end
- fuels internal discussions of recent publications in the field
- sets an example to younger PhD students in the lab
- identifies directions for future scientific research

The position is open immediately and applications will be accepted until the 
position is filled. Applicants are requested to submit a CV with publication 
list and two references. E-mail to chris.ul...@kuleuven.be 


Selected lab publications:

Molecular blueprint of allosteric binding sites in a homologue of the 
agonist-binding domain of the α7 nicotinic acetylcholine receptor. Spurny R, 
Debaveye S, Farinha A, Veys K, Vos AM, Gossas T, Atack J, Bertrand S, Bertrand 
D, Danielson UH, Tresadern G, Ulens C.
Proc Natl Acad Sci U S A. 2015 May 12;112(19):E2543-52.

Pentameric ligand-gated ion channel ELIC is activated by GABA and modulated by 
benzodiazepines. Spurny R, Ramerstorfer J, Price K, Brams M, Ernst M, Nury H, 
Verheij M, Legrand P, Bertrand D, Bertrand S, Dougherty DA, de Esch IJ, 
Corringer PJ, Sieghart W, Lummis SC, Ulens C. Proc Natl Acad Sci U S A. 2012 
Oct 30;109(44):E3028-34.

A structural and mutagenic blueprint for molecular recognition of strychnine 
and d-tubocurarine by different cys-loop receptors. Brams M, Pandya A, Kuzmin 
D, van Elk R, Krijnen L, Yakel JL, Tsetlin V, Smit AB, Ulens C.
PLoS Biol. 2011 Mar;9(3):e1001034.

Re: [ccp4bb] post-doctoral position and PhD position structural biology of ion channels

[Chris Ulens]

A post-doctoral position and PhD position are available at the laboratory of 
Structural Neurobiology, KU Leuven Belgium. The lab website is available at 
http://www.xtal.be. The research in our laboratory is aimed at understanding 
the molecular mechanism of ligand-gated ion channels. Our method relies on 
X-ray crystallography and cryo-EM in combination with electrophysiological 
recordings and fluorescence spectroscopy. Our laboratory hosts an integrated 
macromolecular crystallization facility with a Mosquito crystallization robot 
and two RockImagers. Current structural targets include the family of 
pentameric ligand-gated ion channels (Cys-loop receptors), TRP channels and CNG 
channels.

We would like to recruit a post-doc and/or PhD student with prior experience in 
_expression_, purification and crystallization of membrane proteins. Prior 
experience with X-ray crystallography or cryo-EM is considered a major 
advantage.

The successful candidate:
- easily communicates in an open verbal manner and has a team player spirit
- has an ambitious work ethic
- critically contributes to writing of manuscripts and grant proposals
- makes efforts to obtain independent funding
- meets intermediate deadlines and brings projects to a successful end
- fuels internal discussions of recent publications in the field
- sets an example to younger PhD students in the lab
- identifies directions for future scientific research

The position is open immediately and applications will be accepted until the 
position is filled. Applicants are requested to submit a CV with publication 
list and two references. E-mail to chris.ul...@kuleuven.be

Selected lab publications:

Molecular blueprint of allosteric binding sites in a homologue of the 
agonist-binding domain of the α7 nicotinic acetylcholine receptor. Spurny R, 
Debaveye S, Farinha A, Veys K, Vos AM, Gossas T, Atack J, Bertrand S, Bertrand 
D, Danielson UH, Tresadern G, Ulens C.
Proc Natl Acad Sci U S A. 2015 May 12;112(19):E2543-52.

Pentameric ligand-gated ion channel ELIC is activated by GABA and modulated by 
benzodiazepines. Spurny R, Ramerstorfer J, Price K, Brams M, Ernst M, Nury H, 
Verheij M, Legrand P, Bertrand D, Bertrand S, Dougherty DA, de Esch IJ, 
Corringer PJ, Sieghart W, Lummis SC, Ulens C. Proc Natl Acad Sci U S A. 2012 
Oct 30;109(44):E3028-34.

A structural and mutagenic blueprint for molecular recognition of strychnine 
and d-tubocurarine by different cys-loop receptors. Brams M, Pandya A, Kuzmin 
D, van Elk R, Krijnen L, Yakel JL, Tsetlin V, Smit AB, Ulens C.
PLoS Biol. 2011 Mar;9(3):e1001034.

Re: [ccp4bb] post-doctoral position and PhD position structural biology of ion channels

[Chris Ulens]

A post-doctoral position and PhD position are available at the laboratory of 
Structural Neurobiology, KU Leuven Belgium. The lab website is available at 
http://www.xtal.be. The research in our laboratory is aimed at understanding 
the molecular mechanism of ligand-gated ion channels. Our method relies on 
X-ray crystallography and cryo-EM in combination with electrophysiological 
recordings and fluorescence spectroscopy. Our laboratory hosts an integrated 
macromolecular crystallization facility with a Mosquito crystallization robot 
and two RockImagers. Current structural targets include the family of 
pentameric ligand-gated ion channels (Cys-loop receptors), TRP channels and CNG 
channels.

We would like to recruit a post-doc and/or PhD student with prior experience in 
expression, purification and crystallization of membrane proteins. Prior 
experience with X-ray crystallography or cryo-EM is considered a major 
advantage.

The successful candidate:
- easily communicates in an open verbal manner and has a team player spirit
- has an ambitious work ethic
- critically contributes to writing of manuscripts and grant proposals
- makes efforts to obtain independent funding
- meets intermediate deadlines and brings projects to a successful end
- fuels internal discussions of recent publications in the field
- sets an example to younger PhD students in the lab
- identifies directions for future scientific research

The position is open immediately and applications will be accepted until the 
position is filled. Applicants are requested to submit a CV with publication 
list and two references. E-mail to chris.ul...@kuleuven.be

Selected lab publications:

Molecular blueprint of allosteric binding sites in a homologue of the 
agonist-binding domain of the α7 nicotinic acetylcholine receptor. Spurny R, 
Debaveye S, Farinha A, Veys K, Vos AM, Gossas T, Atack J, Bertrand S, Bertrand 
D, Danielson UH, Tresadern G, Ulens C.
Proc Natl Acad Sci U S A. 2015 May 12;112(19):E2543-52.

Pentameric ligand-gated ion channel ELIC is activated by GABA and modulated by 
benzodiazepines. Spurny R, Ramerstorfer J, Price K, Brams M, Ernst M, Nury H, 
Verheij M, Legrand P, Bertrand D, Bertrand S, Dougherty DA, de Esch IJ, 
Corringer PJ, Sieghart W, Lummis SC, Ulens C. Proc Natl Acad Sci U S A. 2012 
Oct 30;109(44):E3028-34.

A structural and mutagenic blueprint for molecular recognition of strychnine 
and d-tubocurarine by different cys-loop receptors. Brams M, Pandya A, Kuzmin 
D, van Elk R, Krijnen L, Yakel JL, Tsetlin V, Smit AB, Ulens C.
PLoS Biol. 2011 Mar;9(3):e1001034.

Re: [ccp4bb] Coot and pymol 3D on Mac Pro with NVIDIA Quadro FX4500

[Chris Ulens]

Dear ccp4 members,
I am looking for a recommendation from users who still have older generations 
of a Mac Pro with the NVIDIA Quadro FX 4500 stereo card. My question is which 
generation of the Mac Pro... 
http://www.ebay.com/sch/Apple-Mac-Pro/111418/bn_649847/i.html
.. is still compatible with the Quadro FX 4500 stereo and Nuvision emitter? We 
have the 2008 version which still is the stereo workhorse in the lab, but was 
wondering if the 2012 version still has the compatibility?

Thank you and best regards
Chris

[ccp4bb] mutagenesis based on ligand-bound crystal structures

[Chris Ulens]

Dear colleagues,
I am looking for a few example studies in which a complete mutagenesis  
of all residue-ligand interactions has been conducted to evaluate the  
energetic contributions of each individual interaction.

Thank you.
Best regards.
-Chris

---
Chris Ulens, Ph.D.
Lab of Structural Neurobiology
Department of Cellular and Molecular Medicine
Campus Gasthuisberg, ON1
Herestraat 49, PB 601
B-3000 Leuven
Belgium
e   chris.ul...@med.kuleuven.be
t+32 16 330689
f+32 16 345699
w   http://www.xtal.be

Re: [ccp4bb] recommendation for ammonium dihydrogen phosphate cryo

[Chris Ulens]

Hi,
I would like to get recommendations for a proper cryo solution for a  
crystallization hit from the Hampton crystal screen Ammonium di- 
hydrogen phosphate 0.4M. I tried increasing glycerol up to 30% with  
the same ammonium phosphate concentration or increasing glycerol up to  
30% in the presence of 1.3M ammonium phosphate. Both gave iced up  
drops (I only tried quick and dirty tests by dipping a cover glass in  
liquid nitrogen).


Thank you.
-Chris

[ccp4bb] viewing and scoring crystallization drops on the iPad

[Chris Ulens]

Our laboratory has been developing an application to view and score  
crystallization drops on the iPad. We would like to know if  
crystallographers see potential benefits from the functionality of the  
iPad to swipe and pinch through drops. We are looking for specific  
comments from Formulatrix users, but other users are also welcome to  
comment.


http://www.youtube.com/watch?v=LezurNhm0pA


Specific ideas for future development are:
- composition of crystallization buffers on a back-flip of the image  
drop
- back-sync of crystallization scores on the iPad with the image  
database

- emailing a drop image to colleague

Thanks.
-Chris

---
Chris Ulens, Ph.D.
Lab of Structural Neurobiology
Department of Molecular Cell Biology
Campus Gasthuisberg, ON1
Herestraat 49, PB 601
B-3000 Leuven
Belgium
http://www.xtal.be

Re: [ccp4bb] viewing and scoring crystallization drops on the iPad

[Chris Ulens]

I understand you want to use in situ diffraction for assigning scores,  
but I guess it is still useful to visually inspect drop images before  
you decide to go to a synchrotron, no?
So let me rephrase the question and ask whether users see any added  
benefit of using an iPad for decision making on 'going to a  
synchrotron/buying Marcus's PX scanner' or not.


Thank you for the private posts indicating we now have a good excuse  
to buy iPads from research grants. Unfortunately for the PI's, we may  
have to buy them for all lab members now.
Would also be interested to find out if there are any members of the  
crystallography community that have experience in writing iPad apps.


Cheers.
-C

On Apr 18, 2011, at 10:23 AM, ferrer wrote:

I agree with Marcus that the most relevant scoring is the X-ray  
analysis, that can now be done in situ.


Just I would like to mention the G-Rob system we developed for that on  
BM30A at ESRF, now commercialized by NatX-ray (www.natx-ray.com).
With G-Rob, you can not only screen in situ, but also collect complete  
dataset in plate (and also on frozen samples, capillaries, ...)!

See http://www.natx-ray.com/products/clip_IE_March2010.html

Best Regards

JL

PS: G-Rob is available on beamline BM30A at ESRF. Don't hesitate to  
ask for beamtime.


On 04/18/2011 10:00 AM, Marcus Winter wrote:





Dear Chris,


I’m prompted by your posting just to mention the Agilent Technologies
PX Scanner ‘Crystal Challenge’ at:

www.agilent.com/chem/crystalchallenge

Thus, the only really useful assessment, or ‘score’,  of objects  
(putative
crystals) – or crystallisation conditions, is by the actual observed  
diffraction
characteristics... and these preferably directly in situ, in the  
horizontal

crystallisation plate, as achieved in the PX Scanner.


Many Thanks and Best Regards,

Marcus Winter (Agilent Technologies)


ATT1..gif



From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf  
Of Chris Ulens

Sent: 18 April 2011 08:24
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] viewing and scoring crystallization drops on the  
iPad


Our laboratory has been developing an application to view and score  
crystallization drops on the iPad. We would like to know if  
crystallographers see potential benefits from the functionality of  
the iPad to swipe and pinch through drops. We are looking for  
specific comments from Formulatrix users, but other users are also  
welcome to comment.



http://www.youtube.com/watch?v=LezurNhm0pA



Specific ideas for future development are:
- composition of crystallization buffers on a back-flip of the image  
drop
- back-sync of crystallization scores on the iPad with the image  
database

- emailing a drop image to colleague


Thanks.
-Chris

---
Chris Ulens, Ph.D.
Lab of Structural Neurobiology
Department of Molecular Cell Biology
Campus Gasthuisberg, ON1
Herestraat 49, PB 601
B-3000 Leuven
Belgium
http://www.xtal.be



--
Jean-Luc Ferrer
|---|
|Institut de Biologie Structurale J.P. Ebel CEA/CNRS/UJF|
|41 rue Jules Horowitz - 38027 Grenoble cedex 1 - FRANCE|
|tel.: +33 (0)4-38-78-59-10 - fax : +33 (0)4-38-78-51-22|
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Re: [ccp4bb] viewing and scoring crystallization drops on the iPad

[Chris Ulens]

Formulatrix is reading this as well, so it would be useful to get  
objective comments from the  100 people that already watched the  
video on youtube.

-Chris

On Apr 18, 2011, at 12:08 PM, Martin Hällberg wrote:

I see obvious benefits. The Karolinska Institutet has two Formulatrix  
cabinets
in a central facility. We are already thinking of buying iPads for the  
web version

of our ELN system.


On Apr 18, 2011, at 9:23 AM, Chris Ulens wrote:

Our laboratory has been developing an application to view and score  
crystallization drops on the iPad. We would like to know if  
crystallographers see potential benefits from the functionality of  
the iPad to swipe and pinch through drops. We are looking for  
specific comments from Formulatrix users, but other users are also  
welcome to comment.



http://www.youtube.com/watch?v=LezurNhm0pA



Specific ideas for future development are:
- composition of crystallization buffers on a back-flip of the image  
drop
- back-sync of crystallization scores on the iPad with the image  
database

- emailing a drop image to colleague


Thanks.
-Chris

---
Chris Ulens, Ph.D.
Lab of Structural Neurobiology
Department of Molecular Cell Biology
Campus Gasthuisberg, ON1
Herestraat 49, PB 601
B-3000 Leuven
Belgium
http://www.xtal.be

Re: [ccp4bb] post-doc and technician position at KULeuven, Belgium - structural biology of ion channel homologs

[Chris Ulens]

A position is available for a post-doctoral researcher and technician  
at the Laboratory for Structural Neurobiology at the KULeuven, Belgium.


http://www.xtal.be

Our research focus is on ion channel homologs from different classes.  
Our strategy to identify targets for structural studies relies on a  
GFP-based pipeline for pre-crystallization screening and nanobody  
technology for purification of GFP fusion proteins. This pipeline has  
been established for E. coli and insect cells and permit scale-up to  
50 liter cultures in Wave bioreactors. Our laboratory hosts an  
integrated macromolecular crystallization facility with a Mosquito  
crystallization robot, 2 RockImagers and an in-house rotating anode X- 
ray source.  In combination with electrophysiological recordings of  
recombinantly expressed ion channels in Xenopus oocytes we aim to  
understand the relationship between structure, function and  
pharmacology of ion channels.


We have ongoing collaborations with Pharma Inc. to aid the rational  
design of new drugs and to facilitate treatment of disorders related  
to dysfunctional ion channels. Our laboratory is also a participant in  
the FP7 consortium Neurocypres devoted to structure, function and  
disease of cys-loop receptors. http://www.neurocypres.eu. We have  
lively interactions with outstanding groups on campus, including the  
ion channel group of Thomas Voets (TRP channels) and the structural  
biology group of Sergei Strelkov.


Prior experience in cloning, cell culture and protein purification  
techniques is required. Post-docs have the opportunity to combine  
research with limited teaching duties and grow into independent  
research careers if desired.
This call is open to citizens of EMBL member states only. Belgian  
citizens or researchers previously employed at KULeuven are excluded.


To apply for this position please send curriculum vitae, a motivation  
letter and the names of at least two references to: chris.ulens at  
med.kuleuven.be
The position is open immediately and applications will be accepted  
until the position is filled.


Selected publications:
Brams M et al. PLoS Biol (2011) in press
Brams M et al. J Biol Chem (2011) http://www.ncbi.nlm.nih.gov/pubmed/21115477
Ulens C et al. J Med Chem (2009) http://www.ncbi.nlm.nih.gov/pubmed/19331415
Dutertre S, Ulens C et al. EMBOJ (2007) 
http://www.ncbi.nlm.nih.gov/pubmed/17660751
Ulens C et al. Proc Natl Acad Sci U S A. (2006). 
http://www.ncbi.nlm.nih.gov/pubmed/16505382
Ulens C and Siegelbaum SA. Neuron (2003) 
http://www.ncbi.nlm.nih.gov/pubmed/14659094

Re: [ccp4bb] High Rmerge with thin frames

[Chris Ulens]

Compensating like this is of course not the best (go and recollect!)
but still way better than unusable data in the meantime. In the case
Sergei originally described it would at least indicate what the
problem may be. Sergei did not say which detector was used for the
data collection so we don't know if it was a Pilatus or a CCD. Maybe
Sergei can fill us in on the details?



The data that Sergei described were collected by yours truly at
beamline ID14-2 of the ESRF, so detector is a Quantum CCD. I collected
data for another protein crystal a few hours before with 0.5 degree
oscillations - no abnormal statistics here, so I don't suspect cryo or
loop instabilities are the underlying cause as suggested before.
The choice of 0.1 degree oscillations appears somewhat unfortunate in
retrospect, but this is what several programs (Best/mosflm/DNA)
suggested to reduce overlaps. The data were collected for the only
crystal that showed decent diffraction to ~3A out of more than 30-40
screened.

-Chris

[ccp4bb] doctoral or post-doctoral position x-ray crystallography of ion channels

[Chris Ulens]

A position is available for a highly motivated doctoral or post- 
doctoral researcher at the Laboratory for Structural Neurobiology at  
the KULeuven, Belgium. The research in our laboratory is currently  
focused on the structural determination of several classes of  
prokaryotic and eukaryotic homologs of ligand- and voltage-gated ion  
channels. At this point we have several ongoing projects at various  
stages, ranging from promising expression screens to in-house  
diffracting crystals. We share part of our equipment with Dr. Sergei  
Strelkov’s Biocrystallography group, which is fully equipped with an X- 
ray diffractometer (Rigaku/Mar), computer cluster and crystallization  
robotics. Both groups have frequent access to several beamlines at  
European synchrotron facilities.


The successful candidate must be strongly motivated and should possess  
excellent written and oral communication skills. Experience in cDNA  
cloning, cell culture and protein purification techniques are  
required. Prior experience with membrane protein crystallography or a  
background in electrophysiology would be a major advantage.


To apply for this position please send curriculum vitae, a brief  
statement describing technical expertise, future research interests  
and the names of at least two references to: chris.ulens at  
med.kuleuven.be
The position is open immediately. Applications will be accepted until  
the position is filled.


Selected publications of Chris Ulens’s research are:
- Dutertre S, Ulens C, Büttner R, Fish A, van Elk R, Kendel Y, Hopping  
G, Alewood PF, Schroeder C, Nicke A, Smit AB, Sixma TK, Lewis RJ.  
AChBP-targeted alpha-conotoxin correlates distinct binding  
orientations with nAChR subtype selectivity. EMBO J. 2007 Aug  
22;26(16):3858-67.
- Ulens C, Hogg RC, Celie PH, Bertrand D, Tsetlin V, Smit AB, Sixma  
TK. Structural determinants of selective alpha-conotoxin binding to a  
nicotinic acetylcholine receptor homolog AChBP. Proc Natl Acad Sci U S  
A. 2006 Mar 7;103(10):3615-20.
- Ulens C, Siegelbaum SA. Regulation of hyperpolarization-activated  
HCN channels by cAMP through a gating switch in binding domain  
symmetry. Neuron. 2003 Dec 4;40(5):959-70.
- Ulens C, Tytgat J. Functional heteromerization of HCN1 and HCN2  
pacemaker channels. J Biol Chem. 2001 Mar 2;276(9):6069-72.


Most importantly (!!) - according to Wikipedia snip Belgium is well  
known for its cuisine. Many highly ranked restaurants can be found in  
the high-impact gastronomic guides, such as the Michelin Guide.  
Belgian food is, like the country itself, a mix of Germanic and Latin  
influences. Belgians have a reputation for loving waffles and French  
fries; contrary to the name of the latter, both dishes originated in  
Belgium. The national dishes are steak-frites with salad, and moules- 
frites (mussels with frites). Brands of Belgian chocolate and  
pralines, like Callebaut, Côte d’Or, Neuhaus, Leonidas, Guylian and  
Godiva, are world renowned and widely sold. Belgium produces over 500  
varieties of beer. The biggest brewer in the world by volume is InBev  
based in Belgium./snip


Disclaimer: http://www.kuleuven.be/cwis/email_disclaimer.htm

[ccp4bb] nVidia Quadro FX3000 on Ubuntu 8.10

[Chris Ulens]

Hi,
I'm trying to install a nVidia Quadro FX3000 card on a PC running  
Ubuntu 8.10 intrepid.


I run into the following error when I install drivers I downloaded  
from the nVidia site:
The installer fails to find a kernel interface from the nvidia ftp  
site and fails to compile the kernel.


Any help would be greatly appreciated.
Thanks and best wishes.

-Chris

Attached is the complete log file.





Disclaimer: http://www.kuleuven.be/cwis/email_disclaimer.htm



nvidia-installer.log
Description: Binary data




+++
Chris Ulens, Ph.D.
Lab of Structural Neurobiology
Division of Pharmacology
Campus Gasthuisberg, ON1
Herestraat 49, PB 601
B-3000 Leuven
Belgium
+++

Re: [ccp4bb] nVidia Quadro FX3000 on Ubuntu 8.10

[Chris Ulens]

Thanks envyng did the trick, but the emitter doesn't light up when I  
run pymol or coot. There's also no stereo sync on the screen.


I've installed driver version 173.14.12

Thanks.
-Chris


On 07 Jan 2009, at 11:54, Partha Chakrabarti wrote:

Chris,
Try this:

sudo apt-get install envyng
sudo apt-get install envyng-gtk

fire the envyng-gtk interface from the command line or menu and see if
it finds a solution..

HTH, Partha



On Wed, Jan 7, 2009 at 10:49 AM, Chris Ulens
chris.ul...@med.kuleuven.be wrote:

Hi,
I'm trying to install a nVidia Quadro FX3000 card on a PC running  
Ubuntu

8.10 intrepid.

I run into the following error when I install drivers I downloaded  
from the

nVidia site:
The installer fails to find a kernel interface from the nvidia ftp  
site and

fails to compile the kernel.

Any help would be greatly appreciated.
Thanks and best wishes.

-Chris

Attached is the complete log file.





Disclaimer: http://www.kuleuven.be/cwis/email_disclaimer.htm





+++
Chris Ulens, Ph.D.
Lab of Structural Neurobiology
Division of Pharmacology
Campus Gasthuisberg, ON1
Herestraat 49, PB 601
B-3000 Leuven
Belgium
+++







--
MRC National Institute for Medical Research
Division of Molecular Structure
The Ridgeway, NW7 1AA, UK
Email: pcha...@nimr.mrc.ac.uk
Phone: + 44 208 816 2515



Disclaimer: http://www.kuleuven.be/cwis/email_disclaimer.htm

[ccp4bb] postdoc position: structural biology of ion channels

[Chris Ulens]

A position is available for a post-doctoral researcher at the  
Laboratory for Structural Neurobiology at the KULeuven, Belgium. The  
research in our laboratory is currently focused on the structural  
determination of several classes of prokaryotic and eukaryotic  
homologs of ligand- and voltage-gated ion channels. We have ongoing  
collaborations with several ion channel physiology groups on campus,  
including the lab of Dr. Bernd Nilius and Dr. Thomas Voets, who have  
published leading papers in the field of heat/cold- and ligand- 
activated TRP channels. We share part of our equipment with Dr.  
Sergei Strelkov's Biocrystallography group, which is fully equipped  
with an X-ray diffractometer (Rigaku/Mar), crystallization facilities  
and computer cluster.  More information about research at the  
University of Leuven can be found at http://www.kuleuven.be.


The successful candidate must be strongly motivated and should  
possess excellent written and oral communication skills. Experience  
in cDNA cloning, cell culture and protein purification techniques are  
required. Prior experience with membrane proteins would be a major  
advantage.


To apply for this position please send curriculum vitae, a brief  
statement describing technical expertise, future research interests  
and the names of at least two references to:


chris.ulens at med.kuleuven.be

Initial funding is available until September 2010. Applications will  
be accepted until the position is filled.


++
Chris Ulens, Ph.D.
Lab of Structural Neurobiology
Division of Pharmacology
Campus Gasthuisberg, ON1
Herestraat 49, PB 601
B-3000 Leuven
Belgium
E-mail chris.ulens at med.kuleuven.be
Tel +32 16 345812
Fax +32 16 345699
Telepathy 2.69 GHz
++



Disclaimer: http://www.kuleuven.be/cwis/email_disclaimer.htm