Hello Artem,
Yes, we also tried the Expi293 cells when they first came out. They also did
not perform well. When you tested with your generic medium did you use their
transfection reagent or good ol PEI?
I would be very interested to see if over the years if anything has changed as
well.
Hi Kelvin
We never tried with ExpiCHO, but the Expi-293 cells in a generic medium
performed like a geriatric gerbil compared to the Old Faithful in the
actual medium. Transfection was still close to 90% but yield suffered
considerably.
I am sure that with enough diligence the secret sauce can be
Dear Artem,
By work poorly do you mean, not as well and are you referring to all Expi cells?
We have adapted ExpiCHO cells to another medium and although we see drastic
decrease from using the complete Expi system (x15 volumetric yield), it was
still considerably higher than our previous
bulletin board On Behalf Of LAURA DEL AMO
MAESTRO
Sent: Tuesday, March 10, 2020 1:41 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Expi 293 (Met-) expression medium for selenomethionine
labeling
Dear ccp4 community,
I am in my last 3 months of my PhD, working at the Structural Biology Unit
Hello
Bad news: Expi cells work poorly in non-Expi medium :(
Good news: you can add SelenoMet and make partially seleniated protein,
which is usually enough. Note that too much SeMet is pretty toxic, and that
the toxicity is associated with BOTH enantiomers of the amino acid, so if
you have the
h 2020 17:40
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Expi 293 (Met-) expression medium for selenomethionine
labeling
Dear ccp4 community,
I am in my last 3 months of my PhD, working at the Structural Biology Unit at
IBMB-CSIC in Barcelona. As my experimental phasing trials using various h
MD 21201
Tel: (410) 706-7457
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of LAURA DEL
AMO MAESTRO
Sent: Tuesday, March 10, 2020 1:41 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Expi 293 (Met-) expression medium for selenomethionine
labeling
Dear ccp4 community,
I am
Dear ccp4 community,
I am in my last 3 months of my PhD, working at the Structural Biology Unit
at IBMB-CSIC in Barcelona. As my experimental phasing trials using various
heavy metals failed so far, we ordered two months ago Expi 293 (Met-)
expression medium for selenomethionine labeling (see the
