Dear Scott,
By “AND” searching, presumably you mean adding another SEARCH command? (Or
clicking “Add another search” in the ccp4i interface.) For Steven’s strategy,
you want to specify separate searches for separate ensembles, i.e. give several
SEARCH commands in a script or add extra searches
Hi Steven,
Thank you for the information and guidance. When you search for all the
ensembles with Phaser do you use “AND” or “OR”
searching?
Cheers,
Scott
While Phil Jeffrey attributed to me the “trick” of aligning the hinge axis of
an Fab along the Z direction, I, in turn, must give credit
All:
While Phil Jeffrey attributed to me the "trick" of aligning the hinge axis of
an Fab along the Z direction, I, in turn, must give credit to Mirek Cygler, who
explained this to me at the Diffraction Methods in Molecular Biology [now
Structural Biology] Gordon Research Conference in 1986.
Hi Phil,
Thank you for the input. I would like to get CCP4ers input. I deal with
multiple domain
cytokine receptors in a manner very similar to antibody molecules.
Have people have more correct solutions searching for 1 domain sequentially and
then other
domains OR searching for multiple doma
That document is fairly old and is in dire need of revision to reflect
the modern arsenal of programs.
Nevertheless:
Putting the hinge axis along Z was a trick told to me by Steven Sheriff
back in the days when we worked on Fab structures - which after all are
classical examples of hinged mole
Since there is a hinge, you could try searching with the two domains
separately.
On 10/05/14 03:34, Luzuokun wrote:
Dear all,
I’m doing molecular replacement using Phaser. My protein is predicted
to have two domain with a “hinge” linking them. The model sequence
identity is 0.27. But the MR r
Dear all,
I’m doing molecular replacement using Phaser. My protein is predicted
to have two domain with a “hinge” linking them. The model sequence identity is
0.27. But the MR result is poor. I’ve tried other programme (Molrep, MrBump,
Balbes,,,_.) But no improvement was observed. I thin
