Dear Praveen,
I always siliconize my cover slips before using them for crystallization.
Recipe is very simple. But make sure that you always use mask, gloves and
perform your experiment under a fume hood.
1. 95% toluene + 5% dichloromethylsilane will give you a very good
siliconization
@JISCMAIL.AC.UK] Im Auftrag von Tristan
Croll
Gesendet: Freitag, 28. April 2017 13:36
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re: [ccp4bb] Sliconizing of cover-slips
Ahh, this brings back memories of a former life, preparing hydrophobic
coverslips for my surface chemistry experiments. I used
From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Tristan Croll
<ti...@cam.ac.uk>
Sent: 28 April 2017 12:36
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Sliconizing of cover-slips
Ahh, this brings back memories of a former life, preparing hydrophobic
cover
Ahh, this brings back memories of a former life, preparing hydrophobic
coverslips for my surface chemistry experiments. I used chlorotrimethylsilane,
and what I remember best is that the secret to a good coating is making sure
your coverslips are utterly clean and dry. I used to clean them by
[mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Praveen
Kumar Tripathi
Sent: 27 April 2017 10:26
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Sliconizing of cover-slips
Dear all,
sorry for off topic question.
May i know if anybody uses homemade silinization of coverslips for protein
crystallization purposes?
I
Dear all,
sorry for off topic question.
May i know if anybody uses homemade silinization of coverslips for protein
crystallization purposes?
I have purchased Sigmacote SL2-100 ml for silanizing coverslips for hanging
drop protein crystallization setup.
Please share your methods to siliconize
