In short, yes you can. Although it is often avoided due to the limitations
that high salt samples may have, you still have a good chance to
crystallize the protein in the original high salt buffer. In my case a
small alcohol was the best precipitating agent. As with most proteins, try
it, try it,
[ccp4bb] can I try crystallization in high salt?
Dear All,
I have a peri domain protein that is stable in high salt concentration(500
mM), if I dialysis to a lower salt buffer and then concentrate, it'll
preticipate out. If I need to crystallize it, can I use the high salt
buffer
Dear All,
I have a peri domain protein that is stable in high salt concentration(500
mM), if I dialysis to a lower salt buffer and then concentrate, it'll
preticipate out. If I need to crystallize it, can I use the high salt
buffer? Is there any optimization kits that could help to increase the
You can try including some salt to the reservoir after mixing protein
and well solution.
Joe
rui wrote:
Dear All,
I have a peri domain protein that is stable in high salt
concentration(500 mM), if I dialysis to a lower salt buffer and then
concentrate, it'll preticipate out. If I need to
You may also apply salting-in: if the reservoir concentration is lower
than that of the protein buffer, water may evaporate from the reservoir
into the drop, lowering the protein's solubility and thus maybe grow
crystals.
Tim
--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
