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-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of James
Holton
Sent: Tuesday, July 26, 2011 12:56 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] small lysozyme crystals?
Does anyone out there have
James
One thing you should be aware of is that bugs growing in the lysozyme
stock solution can have a dramatic effect on the number and size of
crystals.
(Way, way) back in 1993 when I was in David Blow's lab we noticed an
ageing effect with the lysozyme stock solution; if we dissolved
i wonder if crushing with a hammer maybe a good first step. does
streak seeding after that give smaller crystals amongst the range of
different size crystals possible. perhaps? either way would be good
for the summer student to try out.
--
Karthik
On Tue, Jul 26, 2011 at 10:55 AM, James Holton
As a bit of an aside, my current reference crystal of choice is a Germanium
Oxide with a 51.3Ang cubic cell.
see here:
http://www.nature.com/nature/journal/v437/n7059/full/nature04097.html
It is much more raditation hard than protein so I can do repeated measurements
in the synchrotron beam
Does anyone out there have a protocol of growing HEWL crystals that are
all 50-100 microns wide? I gave this project to a summer student
recently, thinking it would be easy, but it is turning out to be more
difficult than I thought. Keep getting sphereulites instead of small
crystals. Yes,
. This paper will probably give you some ideas in the right
direction.
Mark van der Woerd
-Original Message-
From: James Holton jmhol...@lbl.gov
To: CCP4BB@JISCMAIL.AC.UK
Sent: Tue, Jul 26, 2011 11:55 am
Subject: [ccp4bb] small lysozyme crystals?
Does anyone out there have a protocol
That's a really old paper. You can purchase the lysozyme from Hampton
Research and it's fine. The recipe is available from the Hampton Research
page:
http://hamptonresearch.com/product_detail.aspx?cid=28sid=173pid=524
Grow them a low temp and you can stop them when they are the right size.
I
Use the 15 minute protocol from the Hampton Research catalogue/website. They're normally quite small and easy to cryoprtect with a little glycerol. Size can also be varied by playing with the salt concentration (slightly higher [NaCl]giving more but smaller xtals)
Paul..
Rigaku has a page of common crystallization recipes:
http://www.rigaku.com/protein/crystallization.html
Try the Lysozyme crystallization procedure for low temp use recipe. It
contains ethylene glycol so the crystals are cryo-ready. To get a large
number of smaller crystals, push the concentration
:[EMAIL PROTECTED] On Behalf Of Jacob Keller
Sent: Thursday, July 03, 2008 4:08 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Small lysozyme crystals protocol
Dear Crystallogrphers,
does anybody here know a protocol to get consistently well-diffracting but
smaller, ~50um, cryoprotect(ed/able
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