Dear colleagues,
please be informed that the registration slot for the
9th International NCCR Symposium on New Trends in Structural Biology
1 + 2 September 2011, University of Zurich, Irchel Campus, Lecture Hall Y24-G-45, Zurich, Switzerland
is now open. Online registration is
Hi folks,
crystallized a lot of SRP RNAs - never had success with synthetic RNA,
even with small 30mers T7 RNA pol in vitro transcribed was always
extremely better - moreover: I always use a 3' hammerhead which yields
in the end 100% pure material in mg amounts - Kiyoshi Nagais protocol is
a
Dear CCP4BB
Thank you to everyone who sent me answers to my problem. I obtained the
average B factors using Baverage. It was also suggest that I could have used
act, a script from the CCP4 homepage, MOLEMAN, phenix.model_vs_data or
copying the data into Excel using the paste special option and
On 03/12/2011 05:29 AM, Vandu Murugan wrote:
Dear all,
Recently I collected a data for my 20Kda protein in C2 space group, and
ran SFcheck in ccp4 suite. It is giving an indication for
pseudo-translation as 'Pseudo-translation is detected: 17.6%
Pseudo-translation vector: 0.000
Dear all,
Sorry for this off topic question.
We are working on protein/inhibitor complex structure although we can not get
our inhibitors in. However, we did find a strange density at the active site,
it looks really like GSH, the natural co-enzyme of thiis protein.We tried to
use very simple
Dear CCP4bb,
I am trying to solve the structure of a protein-protein complex. The two
proteins are co-expressed and co-purified and I get a homogeneous 1:3
complex, which is the predicted composition. I get several hits using
commercial crystallisation screens (including the MD ProPlex
Dear Catrine
can you comment on the pH range of your crystallization hits compared to the pH
of the sample you use for your crystallization trials? The pH can be a major
issue depending on the nature of the interaction interface.
best regards
Savvas
On 14 Mar 2011, at 13:21, Catrine
Dear All,
CCP4 is happy to announce 3 open vacancies for Scientific Programmer positions
at CCP4 core team. Last year, the team has relocated to the newly built
Research Complex at Harwell (RCaH), Rutherford Appleton Laboratory (RAL) near
Oxford, UK, and is now seeking to expand. CCP4 has
Hi Michael,
If the problem is the absence of a purine on the 5' end, try using the Marc
Dreyfus' mutant T7 RNA polymerase. It can accommodate any nucleotide at the 5'
end.
Guillerez, J., Lopez, P. J., Proux, F., Launay, H., and Dreyfus, M. (2005) A
mutation in T7 RNA polymerase that
The X6A workbench: Advanced Structural Biology Tools??
winter session
(http://protein.nsls.bnl.gov)??
Location: NSLS
Date: March 29 through April 1
We would like to invite you to participate in this four day hands-on course at
the National Synchrotron Light Source. This course is intended for
Dear Users,
The deadline for May/June 2011 Collaborative
Crystallography proposals will be *Mar 15, 2011.*
Through the Collaborative Crystallography Program (CC) at the
Advanced Light Source (ALS),
Dear Users,
The deadline for May/June 2011 Collaborative
Crystallography proposals will be *Mar 15, 2011.*
Through the Collaborative Crystallography Program (CC) at the
Advanced Light Source (ALS),
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