Re: [ccp4bb] Solubility of Aluminum Fluoride

I have never tried varying the order of mixing, but it seems the correct ADP-BeF3 complex was formed when mixing all three solutions just before use. Guillaume > On 22 Jun 2022, at 02:12, Firdous Tarique wrote: > > Thanks for your input. To avoid precipitation what about doing something like

Re: [ccp4bb] Patterson map

Can you send your command file ? And how are you displaying the Patterson map ? >From CCP4I you can request a peak search of the map - check if the peaks correspond to ones you would expect from your coordinates Maybe read the map and vector positions into COOT to see? Eleanor On Tue, 21 Jun

Re: [ccp4bb] Solubility of Aluminum Fluoride

Sorry, I should have said ADPBeF3 (not ADPBeF4), and increase the NaF proportion for ADPAlF4. Which in practice means reducing the concentrations of everything else due to limited solubility of NaF. From: CCP4 bulletin board on behalf of Dr. Kevin M Jude Date: Wednesday, June 22, 2022 at

Re: [ccp4bb] Solubility of Aluminum Fluoride

Kevin and Guillaume said it all quite well. I just want to stress one thing, namely that these solutions are metastable, and will eventually precipitate (hours to days). In the case of the beryllium salts, the precipitation is due to slow formation of metal hydroxide species. I have no direct

Re: [ccp4bb] Patterson map

Hi, from the FFT documentation: AXIS  *In general DO NOT change the axis order unless you are in P1 or are very sure you know what you are doing! This is permitted if your map is being calculated using the routines for P1 or P222,

Re: [ccp4bb] Solubility of Aluminum Fluoride

I posted the following to ccp4bb in 2017 in response to a similar question about ADPBeF4. For AlF4 you’ll increase the NaF stoichiometrically: reagent[stock] (mM)final concentration (mM)volume (µl) BeCl2 100090 9 NaF750

[ccp4bb] Instruct-ERIC online training course "Instruct-ing Structural Biologists Towards Integration"

Dear all, Instruct-ERIC offers a range of workshops delivered at Instruct Centres ( https://instruct-eric.eu/instruct-training-events-annual). In this framework, the CERM/CIRMMP Italy centre is organizing the online course "Instruct-ing Structural Biologists Towards Integration" - from July 18th

[ccp4bb] open review?

Greetings all, I'd like to ask a question that I expect might generate some spirited discussion. We have seen recently a groundswell of support for openness and transparency in peer review. Not only are pre-prints popular, but we are also seeing reviewer comments getting published along

Re: [ccp4bb] open review?

Dear James, I think open reviews would be a major improvement in the grants review process. Most grant reviews are done carefully and honestly, but I have seen some that were clearly written carelessly and dishonestly that would not have been submitted if the reviewers knew they would have to

Re: [ccp4bb] open review?

Hi James, I agree that it’sa good suggestion. In Sweden when you apply to any of the publicly funded grant agencies the application is automatically in the public domain. This means that anyone can ask to see your proposal and the agency is obliged to send it out to the people asking for it.