Re: [ccp4bb] Difficult purification with imac columns

Dear Narayanan Which kind of resin are you using ? In my lab we work with proteins that bind dextran which is often used as crosslinker in chromatography resins. Crosslinked resins have a highly reticulated structure made of sugars that can be quite similar to peptidoglycan... As a test, you

Re: [ccp4bb] High R/Rfree after MR

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[ccp4bb] High R/Rfree after MR

Dear All, I am trying to refine a structure at 3.3A. Model has 60% identity to the target. Maps look OK (for 3.3A) and rebuilding in Coot is relatively straightforward. However, after some rebuilding cycles the R factors are stuck at 0.37/0.39 (REFMAC). XTRIAGE tells me that everything is normal

[ccp4bb] cruickshanccsk DPI for low resolution structures

Dear All, I think this topics has been already treated many times but, knowing that the majority of structures in the pdb have a DPI around ~0.15 I would like to know what is the consensus value that is commonly defined as "acceptable", especially for low resolution structures (3.5A which could

Re: [ccp4bb] the purification process of protein, used for crystallization

Dear All The ion exchange has the great advantage ,over other tecniques, to concentrate the protein. Histrap+sec is a big classic in protein purification but times it is worth considering other schemes. Why doing a SEC? It is for removing aggregates or a contaminant ? It the latter case probably

[ccp4bb] Hifliq columns

Dear All, Is anybody using the HifliQ columns Ni/Co from Generon ? How well they compare with the other brands ? Thank you, Gianluca

Re: [ccp4bb] Structure of dextran sulfate

Hi, Pure dextrans are linear alpha16 linked. Branched or not depends on the enzyme (dextransucrase) that has been used to synthesize it. If branching occurs this will be mostly on the alpha13. Sulfation can occur on some or all the free hydroxyls groups. Best regards Gia Il 28 Feb 2018 16:47,

Re: [ccp4bb] Off topic: room temp FPLC column cooling

To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 -- 1st French Congress on Integrative Structural Biology Please check: http://bsi-2019.ipbs.fr Dr. Gianluca CIOCI BioCatalysis Team

[ccp4bb] suggestions on small proteins that for a complex

). Is anybody able to give me some suggestions ? Thank you, GIA -- 1st French Congress on Integrative Structural Biology Please check: http://bsi-2019.ipbs.fr Dr. Gianluca CIOCI Toulouse Biotechnology Institute (TBI) BioCatalysis Team http://www.toulouse-biotechnology-institute.fr/en/research

[ccp4bb] Fwd: [ccp4bb] suggestions on small proteins that for a complex

, if possible, I would prefer monomeric proteins (although not really mandatory). Is anybody able to give me some suggestions ? Thank you, GIA -- 1st French Congress on Integrative Structural Biology Please check: http://bsi-2019.ipbs.fr Dr. Gianluca CIOCI Toulouse Biotechnology Institute (TBI

Re: [ccp4bb] twin, pseudosymmetry and NCS in P2/C2 ?

.b.coo...@protonmail.com Sent from Proton Mail mobile Original Message On 21 Mar 2023, 16:43, Gianluca Cioci < 8d6e5314cb4c-dmarc-requ...@jiscmail.ac.uk> wrote: Dear All, I have collected a dataset from a s

[ccp4bb] twin, pseudosymmetry and NCS in P2/C2 ?

nks in advance for any advice on how to rescue these data ! Cheers, GIA Click to zoom the image -- Dr. Gianluca CIOCI Toulouse Biotechnology Institute (TBI) http://www.toulouse-biotechnology-institute.fr/en/research/enzyme-molecular-engineering-and-catalysis/cimes.html PICT - Plateforme Intég

Re: [ccp4bb] Crystallizing a tough target

.* To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 <https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1> -- Dr. Gianluca

[ccp4bb] problem in sugar refinement with CCPEM

  is missing in the structure and interestingly, the Coot version coming with CCPEM (0.9.8.5) is also not able to refine my sugar... However, If I use CCPEM with the older CCP4-7.1, the refinement works fine. Any idea ? With many thanks, Gia -- Dr. Gianluca CIOCI Toulouse Biotechnology