Dear All,
Thanks for the reply.
I will try process and refine the data again, will see if it improves.
Thanks again to all.
--
Sonali Dhindwal
“Live as if you were to die tomorrow. Learn as if you were to live forever.”
From: Mark van Raaij
There isnt much information here!
Funny that 3 chains are poor - does that mean one and a half heterodimers?
I presume you have checked spacegroup? Zanuda will test to see if any
higher symmetry is present..
Eleanor
On 29 April 2013 06:02, sonali dhindwal sonali11dhind...@yahoo.co.inwrote:
Dear Eleanor,
Thanks for the suggestion, I just checked on the Zanuda program, it is also
giving P1 as the best possible spacegroup for the molecule.
and by not refining well, I meant for the electron density which is broken at
many places at main chain, and poor electron density for the
FYI, I do know of one example of a solved structure where some of the
molecules in the ASU are poorly defined. In 1EKJ, 4 of the 8 molecules
in the ASU have low B-factors (mid 30s) and 4 have high B-factors
(50s-60s). In the unit cell these layers alternate. It is possible, if
everything else
You may also get some insights from TLS refinement.
Regards
Thierry
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Roger
Rowlett
Sent: Monday, April 29, 2013 12:27 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Poor electron density in some of the chains in an
Dear All,
Thanks for the help and suggestion
Francis, we used molrep for the structure solution. As I told that we already
have the structure of the same protein, but a variant. So, we used its single
chain (heteromer) as a model for molecular replacement.
and Eleanor, I deleted once that
On 04/29/13 12:26, Roger Rowlett wrote:
FYI, I do know of one example of a solved structure where some of the
molecules in the ASU are poorly defined. In 1EKJ, 4 of the 8 molecules
in the ASU have low B-factors (mid 30s) and 4 have high B-factors
(50s-60s). In the unit cell these layers
and 2VAK...average Bs for the twelve, sequence identical, chains vary from 28
to 53.
On 29 Apr 2013, at 22:09, David Schuller wrote:
On 04/29/13 12:26, Roger Rowlett wrote:
FYI, I do know of one example of a solved structure where some of the
molecules in the ASU are poorly defined. In
