Re: [galaxy-dev] SGE and Galaxy (a different approach)

2011-04-05 Thread Bram Slabbinck
Hi andrew What you need to do is to add the qsub parameter '-sync y'. This puts a hold on the qsub command and makes it wait until the SGE job is finished. regards Bram On 05/04/2011 18:27, andrew stewart wrote: I'm aware of how to configure Galaxy to use SGE in universe_wsgi.ini, however wh

Re: [galaxy-dev] Ratings on Tool Shed broken?

2011-04-05 Thread Florent Angly
Hi Peter, I get the same error here. Florent On 06/04/11 01:50, Peter Cock wrote: Hi all, I had wondered why none of the tools on the Galaxy Tool Shed had a rating, and had assumed it was just that so far no-one had bothered. I have a new theory, it doesn't work? I just viewed Brad's "BAM to

[galaxy-dev] samtools sam-to-bam problem

2011-04-05 Thread Ryan Golhar
I've performed an alignment using BWA on a file of paired-end illumina reads. The SAM file looks fine, and contains header information. I'm converting it to BAM using the sam to bam converter, however it consistently errors out after running for a while. The error is: "Error extracting alig

Re: [galaxy-dev] Freebayes

2011-04-05 Thread Juan Carlos Perin
I just tried via command line and re-did the steps that Galaxy attempted on the same files. I got through the samtools sorting of the bam file without a problem, but then soon saw the same error when I tried executing freebayes itself: [perin@variome 000]$ freebayes --fasta-reference /share/a

[galaxy-dev] tmp directory not cleaned up?

2011-04-05 Thread Ryan Golhar
I just noticed a lot of files in my galaxy tmp directory. Since this isn't a system tmp directory, the system cron scripts don't clean it up. Is there a galaxy cron script that can be used to clean up this directory? -- CONFIDENTIALITY NOTICE: This email communication may contain private, con

Re: [galaxy-dev] Optimizing Disk space using by combining mapping with SAM-to-BAM

2011-04-05 Thread Ryan Golhar
Hi Gordon - It looks like on the samtools mailing lists there is an active discussion on speeding up sorting: http://sourceforge.net/mailarchive/message.php?msg_id=27247076 http://sourceforge.net/mailarchive/message.php?msg_id=26990598 Interestingly enough there is a recommendation on using Pic

Re: [galaxy-dev] Optimizing Disk space using by combining mapping with SAM-to-BAM

2011-04-05 Thread Assaf Gordon
Hello Ryan, I'm in the exact same situation with my bowtie/tophat tools, going back and forth between outputing a SAM, sorted SAM, BAM or sorted BAM, and I'm still not sure what's the best method. Storage wise - you're correct, just saving the sorted BAM is the best (even more with the fact the

[galaxy-dev] Optimizing Disk space using by combining mapping with SAM-to-BAM

2011-04-05 Thread Ryan Golhar
Hi all - I find it redundant to hold on to SAM output from NGS Mapping tools such when I end up converting the SAM files to BAM files anyway. The cleanup scripts require the history items to be deleted, but I don't want to delete them yet as I want the entire workflow to be kept until we are do

Re: [galaxy-dev] SGE and Galaxy (a different approach)

2011-04-05 Thread Sean Davis
On Tue, Apr 5, 2011 at 12:27 PM, andrew stewart wrote: > I'm aware of how to configure Galaxy to use SGE in universe_wsgi.ini, > however what I want to do is a little different. Hi, Andrew. Take a look at this page: https://bitbucket.org/galaxy/galaxy-central/wiki/Config/Cluster In particular,

Re: [galaxy-dev] SGE and Galaxy (a different approach)

2011-04-05 Thread Nate Coraor
andrew stewart wrote: > I'm aware of how to configure Galaxy to use SGE in universe_wsgi.ini, > however what I want to do is a little different. Because I only want > certain processes to be submitted to the queue, I'd rather control this at > the tool configuration level (the xml wrapper). For e

Re: [galaxy-dev] SGE and Galaxy (a different approach)

2011-04-05 Thread Luobin Yang
Use qrsh instead of qsub to submit the job should help, I believe. On Tue, Apr 5, 2011 at 10:27 AM, andrew stewart wrote: > I'm aware of how to configure Galaxy to use SGE in universe_wsgi.ini, > however what I want to do is a little different. Because I only want > certain processes to be submi

Re: [galaxy-dev] SGE and Galaxy (a different approach)

2011-04-05 Thread andrew stewart
Ah, I should have added that this is for a strictly in-house Galaxy implementation with absolutely no intention of publishing these specific tools (just these ones! I promise I'll publish other tools in the future :) On Tue, Apr 5, 2011 at 12:38 PM, Peter Cock wrote: > On Tue, Apr 5, 2011 at 5:

Re: [galaxy-dev] SGE and Galaxy (a different approach)

2011-04-05 Thread Peter Cock
On Tue, Apr 5, 2011 at 5:27 PM, andrew stewart wrote: > I'm aware of how to configure Galaxy to use SGE in universe_wsgi.ini, > however what I want to do is a little different.  Because I only want > certain processes to be submitted to the queue, I'd rather control this at > the tool configuratio

[galaxy-dev] SGE and Galaxy (a different approach)

2011-04-05 Thread andrew stewart
I'm aware of how to configure Galaxy to use SGE in universe_wsgi.ini, however what I want to do is a little different. Because I only want certain processes to be submitted to the queue, I'd rather control this at the tool configuration level (the xml wrapper). For example: qsub myscript.sh

[galaxy-dev] Ratings on Tool Shed broken?

2011-04-05 Thread Peter Cock
Hi all, I had wondered why none of the tools on the Galaxy Tool Shed had a rating, and had assumed it was just that so far no-one had bothered. I have a new theory, it doesn't work? I just viewed Brad's "BAM to BigWig" tool, clicked on "Tool Actions" then "Rate Tool", and got: Server Error An er

Re: [galaxy-dev] Missing Page Editor Components

2011-04-05 Thread charlie
Great. I will check out, thanks! On Thu, Mar 17, 2011 at 2:46 PM, Jeremy Goecks wrote: > > However, when I tried to use the Edit Pages function, there is no "Embed >> Galaxy Object" and "Insert Link to Galaxy Object" in the "Page Editor". I >> have set the "enable_pages = True" in "universe_wsgi.

[galaxy-dev] Trouble viewing interval files in UCSC with Apache+XSendFile

2011-04-05 Thread Assaf Gordon
Hello, I've encountered a strange combination of factors that results in file access problems. Perhaps I'm doing something wrong - any advice will be appreciated. I'm using Apache + XSendFile. when viewing a strict BED file, everything works, because the actual dataset filename is passed on to

Re: [galaxy-dev] Updating BLAST+ wrapper

2011-04-05 Thread Peter Cock
On Mon, Apr 4, 2011 at 6:07 PM, Peter Cock wrote: > On Mon, Apr 4, 2011 at 5:55 PM, Kanwei Li wrote: >> Done the manual way >> > > Thanks. > > I was hoping to have finished this today, but it turns out there are > some subtle but annoying changes to how BLAST records the hit > id/name/description

Re: [galaxy-dev] Ignoring white space differences in test output

2011-04-05 Thread Peter Cock
On Tue, Apr 5, 2011 at 10:58 AM, Peter Cock wrote: > Hi all, > > I'd like to write a tool test where the sample output and the > generated output are the same except for white space. > i.e. running diff with the -w switch reports no differences. > > Is this possible at the moment with the Galaxy t

Re: [galaxy-dev] Freebayes

2011-04-05 Thread Dannon Baker
Juan, I'm not sure of a likely cause of this error off the top of my head. Have you tried running Freebayes from the command line on the same data input? That might be an easy way to explore what particular options with the newer version of Freebayes that you have are causing issues, or perha

[galaxy-dev] Ignoring white space differences in test output

2011-04-05 Thread Peter Cock
Hi all, I'd like to write a tool test where the sample output and the generated output are the same except for white space. i.e. running diff with the -w switch reports no differences. Is this possible at the moment with the Galaxy test framework? I'm hoping for a attribute. I don't see anythin