[galaxy-dev] Genome not listed

2013-05-29 Thread sridhar srinivasan
Dear developers, 1. I recently installed galaxy locally. now i am trying to run bowtie2. In reference genome section the genome is not listed. 2. Is there any way yo secure our data without using the Proxy(nginx). I Appericiate your suggestions. Thanks Sridhar

[galaxy-dev] installing galaxy

2013-05-29 Thread מירב .
hello, I am installing galaxy locally. I tried to upload a .bam file and I got this message: Traceback (most recent call last): File /home/user/galaxy_project/tools/data_source/upload.py, line 386, in module __main__() File /home/user/galaxy_project/tools/data_source/upload.py, line

Re: [galaxy-dev] installing galaxy

2013-05-29 Thread Ross
Hi, Merav. A fully functional Galaxy server relies on a lot of third party software and the error message you see is probably because you do not have samtools available - the upload process needs it to make an index for your bam file. So the solution may be to install the samtools package - eg for

Re: [galaxy-dev] Creating multiple datasets in a libset

2013-05-29 Thread Dannon Baker
Has the script created multiple histories? That's the intent, that each file+workflow execution has a separate history. And, for why that file vanished resulting in the exception -- is it possible you ran a second instance of the script or moved the file? -Dannon On Wed, May 29, 2013 at 1:07

[galaxy-dev] php script integration query

2013-05-29 Thread Prasun Dutta
Hi, If I have some programs in php which I need to integrate in Galaxy as a tool, can I do it? I know if I have some programs written in Perl, python,java,C or C++, I can easily integrate them. Can I do the same with a php script?   Regards, Prasun Dutta MSc (Bioinformatics) School of

Re: [galaxy-dev] php script integration query

2013-05-29 Thread Dannon Baker
As long as you're able to execute your php script from the command line, it should work fine. -Dannon On Wed, May 29, 2013 at 7:25 AM, Prasun Dutta prasundutt...@yahoo.co.inwrote: Hi, If I have some programs in php which I need to integrate in Galaxy as a tool, can I do it? I know if I

Re: [galaxy-dev] advice on a mixed cloud/SGE architecture

2013-05-29 Thread Dannon Baker
Ok, got it. Unfortunately this isn't really possible at this time, at least not without a lot of hacking and dealing with data staging issues and delays (in downloading data from S3 to local cluster to work, uploading results back to galaxy server, etc.). Galaxy, with the exception of the LWR -

[galaxy-dev] cufflinks in the main galaxy instance is not able to produce compatible FPKM score

2013-05-29 Thread Nikolay N.
Hi, Most people using the RNA-Seq toolkit use a combination of Tophat/Cufflinks/Cuffdiff. A known issue is that cufflinks and cuffdiff use different normalisation schemes ( http://seqanswers.com/forums/archive/index.php/t-26041.html). Users overcome this problem by running cufflinks with the

Re: [galaxy-dev] Creating tool shed repository: installation of dependencies fails.

2013-05-29 Thread Dave Bouvier
Joachim, I'm unable to duplicate that error, what revision of Galaxy and the tool shed code are you running? The path mentioned in the error message is a temporary working directory that is created for downloading and processing files needed for a tool dependency. Had the installation

[galaxy-dev] Feature Request - Reload Button

2013-05-29 Thread Michael E. Cotterell
I noticed that if I touch universe_wsgi.ini from the terminal (update its timestamp) while Galaxy is running then Galaxy restarts. If this is intended, would it be possible to add a Reload Galaxy button/option in the Server section of the Admin page that mimics this feature? Obviously, you'd

Re: [galaxy-dev] Run galaxy on a specific SGE queue

2013-05-29 Thread Nate Coraor
On May 22, 2013, at 11:36 AM, Sarah Maman wrote: Hi everyone, Galaxy is set up on our cluster, and so far everything is working just fine. However, we'd like to change the submission queue from the default to a specific one. To do so, we tryed to set on the universe_wsgi.ini file the

Re: [galaxy-dev] Creating tool shed repository: installation of dependencies fails.

2013-05-29 Thread Joachim Jacob | VIB |
Strange. When I just wget that link in a terminal, do 'chmod +X blat' and ./blat, it just works fine. Could it be that the permissions need to be set by Galaxy? Version info of the Tool Shed: * [galaxy@galaxy galaxy-dist]$ hg summary parent:

Re: [galaxy-dev] Creating multiple datasets in a libset

2013-05-29 Thread Rob Leclerc
Hi Neil, I've attached my class function for uploading multiple files. def upload_files(self, fullpaths): Uploads files from a disk location to a Galaxy library Accepts an array of full path filenames Example: fullpaths = ['/home/username/file1.txt',

Re: [galaxy-dev] Creating tool shed repository: installation of dependencies fails.

2013-05-29 Thread Joachim Jacob | VIB |
The problem is most likely in ~/lib/tool_shed/galaxy_install/tool_dependencies/fabric_util.py, line 100. 1. current_dir = os.path.abspath( os.path.join( work_dir, dir ) ) 2. while dir is set a couple of lines before as: dir = workdir (line 78). workdir is the temporary directory. 3.

[galaxy-dev] organizing datasets in the history

2013-05-29 Thread 吳正華
Hi, galaxy community: I’ve set up a local galaxy for our institute, one thing that our users kept complaining is that when dealing with several dozens of datasets, it became hard to find a certain data. I’m wondering is there a way to organize data in history? Say, organizing datasets in

Re: [galaxy-dev] cufflinks in the main galaxy instance is not able to produce compatible FPKM score

2013-05-29 Thread Jeremy Goecks
These are options we'd like to add to Cufflinks, and I've created a Trello card for this enhancement: https://trello.com/c/U7nceKdj I want to add that community contributions via patch or pull request are highly encouraged and are likely to be quicker than waiting for the core team to get to

Re: [galaxy-dev] organizing datasets in the history

2013-05-29 Thread Jeremy Goecks
When a user creates an account, s/he can create/copy/delete histories and copy datasets from history to history to organize data. Each history, then, is a like a library folder. Best, J. On May 29, 2013, at 11:02 AM, 吳正華 wrote: Hi, galaxy community: I’ve set up a local galaxy for our

[galaxy-dev] Fwd: installing galaxy

2013-05-29 Thread Ross
-- Forwarded message -- From: מירב . mer...@gmail.com Date: Wed, May 29, 2013 at 8:40 PM Subject: Re: [galaxy-dev] installing galaxy To: Ross ross.laza...@gmail.com It worked!! Thank you very mach [?] Merav 2013/5/29 Ross ross.laza...@gmail.com Hi, Merav. A fully functional

[galaxy-dev] Storage question for Galaxy-Admins

2013-05-29 Thread Raj Ayyampalayam
Hello, I am trying to come up with a number for our local galaxy storage requirements when we go live (This is an installation for research at our university). I checked out the survey that was done a while back for the GalaxyAdmins. It seems that the bigger installations have allocated

Re: [galaxy-dev] installing galaxy

2013-05-29 Thread מירב .
It worked!! Thank you very mach Merav 2013/5/29 Ross ross.laza...@gmail.com Hi, Merav. A fully functional Galaxy server relies on a lot of third party software and the error message you see is probably because you do not have samtools available - the upload process needs it to make an