Re: [galaxy-dev] Regarding galaxy wrappers: lumi, DESeq edgeR

2013-07-08 Thread Ross
(Sachit, please don't cross-post to the dev and user lists?) There's a beta version of an edgeR/deseq/voom wrapper and a companion htseq based count matrix maker in the test toolshed statistics section owned by me - fubar. Definitely not quite ready for production but installable and I'd

[galaxy-dev] Galaxy Server Processes Dying?

2013-07-08 Thread Dan Sullivan
Hi, Galaxy Developers, I have what I'm hoping is a fairly simple inquiry for the Galaxy community; basically, our production Galaxy server processes appear to be dying off over time. Our production Galaxy instance implements apache web scaling features so I have a number of server processes,

Re: [galaxy-dev] Regarding galaxy wrappers: lumi, DESeq edgeR

2013-07-08 Thread Sachit Adhikari
Sorry about that Ross. I would try your wrapper of edgeR and would happily report you the bug. Can you make me the link of Galaxy Test Toolshed? Thanks, Sachit On Mon, Jul 8, 2013 at 11:51 AM, Ross ross.laza...@gmail.com wrote: (Sachit, please don't cross-post to the dev and user lists?)

Re: [galaxy-dev] Regarding galaxy wrappers: lumi, DESeq edgeR

2013-07-08 Thread Ross
Hi Sachit, It's packaged up so it installs from the test toolshed directly via the admin interface of your test instance, but you'll need a good reliable network as there is a lot that gets downloaded and installed. Like other toolshed tools, it would be possible but not advisable to download

Re: [galaxy-dev] [galaxy-user] Regarding galaxy wrappers: lumi, DESeq edgeR

2013-07-08 Thread Björn Grüning
Hi Sachit, we are working currently on a DESeq2 wrapper. Not yet finised sorry. There is also a edgeR Test repository from Ross Lazarus under: http://testtoolshed.g2.bx.psu.edu/view/fubar/edger_test Work is in progress, help is welcome! Ciao, Bjoenr Hi, These are the R libraries. Have

Re: [galaxy-dev] [galaxy-user] Regarding galaxy wrappers: lumi, DESeq edgeR

2013-07-08 Thread Sachit Adhikari
Thank you Björn and Ross. I will install the tools from the admin interface. On Mon, Jul 8, 2013 at 12:35 PM, Björn Grüning bjoern.gruen...@pharmazie.uni-freiburg.de wrote: Hi Sachit, we are working currently on a DESeq2 wrapper. Not yet finised sorry. There is also a edgeR Test

Re: [galaxy-dev] Galaxy Server Processes Dying?

2013-07-08 Thread Ross
Hi Dan, That's old code. Updating will probably help. Logging level just takes disk space, but just in case you haven't followed http://wiki.galaxyproject.org/Admin/Config/Performance/ProductionServer?action=showredirect=Admin%2FConfig%2FPerformance leaving debug = True uncommented used to fill

Re: [galaxy-dev] gzipped fastq reader

2013-07-08 Thread Peter Cock
On Thu, Jul 4, 2013 at 9:49 PM, Robert Baertsch robert.baert...@gmail.com wrote: Dan, Do these readers support gzip files? reader = fastqVerboseErrorReader reader = fastqReader Presumably you are writing a Python script using this library? The answer is a qualified yes.

Re: [galaxy-dev] [GSoC2013] Week 2 Accomplishments and Week 3 Plans

2013-07-08 Thread Saket Choudhary
Week 3 and Week 4 Plans: http://galaxy-gsoc2013.blogspot.in/2013/07/week-3-accomplishments-and-week-4-plans.html On 6 July 2013 16:46, Saket Choudhary sake...@gmail.com wrote: Here is a short post explaining the data model. Not ver elaborately written, so incase you you find a mistake or

Re: [galaxy-dev] CloudMan Error

2013-07-08 Thread greg
Hi guys, Any thoughts on this? I'm kind of stuck. (Even some pointers on where to look for more clues would be extremely helpful.) Thanks, Greg On Fri, Jul 5, 2013 at 11:10 AM, greg margeem...@gmail.com wrote: Hi guys, I'm hitting an error using CloudMan using the Share-an-Instance

Re: [galaxy-dev] RFC: Cheetah template in tool_dependencies.xml

2013-07-08 Thread James Taylor
It probably makes sense to specify the template type in this case. Maybe an additional attribute, language='cheetah' ? On Sun, Jul 7, 2013 at 1:19 PM, Björn Grüning bjoern.gruen...@pharmazie.uni-freiburg.de wrote: action type=template_command #if env.get('X11_LIB_DIR', False) and

Re: [galaxy-dev] RFC: Cheetah template in tool_dependencies.xml

2013-07-08 Thread Björn Grüning
Hi James, It probably makes sense to specify the template type in this case. Maybe an additional attribute, language='cheetah' ? My first suggestion was action type=cheetah_command to reflect the template engine. I'm fine with both. On Sun, Jul 7, 2013 at 1:19 PM, Björn Grüning

Re: [galaxy-dev] How were liftover and nib files generated?

2013-07-08 Thread James Taylor
liftOver files come from the UCSC genome browser, derived from pairwise alignments. A variety of tools can create nib files from fasta, however we prefer the use of the twoBit format now, which can be created using faToTwoBit from the UCSC utilities. -- James Taylor, Assistant Professor,

Re: [galaxy-dev] RFC: Cheetah template in tool_dependencies.xml

2013-07-08 Thread Greg Von Kuster
I'll soon be committing a slightly altered version of your patch which includes a language attribute for the template_command action type. On Jul 8, 2013, at 10:02 AM, Björn Grüning bjoern.gruen...@pharmazie.uni-freiburg.de wrote: Hi James, It probably makes sense to specify the template

[galaxy-dev] Error Uploading Directory of Files

2013-07-08 Thread Nicholas Kline
Hi, Our lab recently installed a local version of Galaxy on a mid-2012 Mac Pro computer. We can access the Galaxy server and sign in as an administrator. Today we tried creating a Data Library, adding a dataset to it, and uploading a directory of files. We followed this Galaxy documentation (

Re: [galaxy-dev] Error Uploading Directory of Files

2013-07-08 Thread Peter Cock
On Mon, Jul 8, 2013 at 3:36 PM, Nicholas Kline nx...@case.edu wrote: Hi, Our lab recently installed a local version of Galaxy on a mid-2012 Mac Pro computer. We can access the Galaxy server and sign in as an administrator. Today we tried creating a Data Library, adding a dataset to it, and

[galaxy-dev] ToolShed dependencies KeyError: 'invalid_repository_dependencies'

2013-07-08 Thread Peter Cock
Hi Greg, Previously my workflows had hard coded dependencies on the main Tool Shed, which currently is a bit of a problem on the Test Tool Shed. Therefore I'm update my Tool Shed workflows using a repository_dependencies.xml with implicit repository and revision fields (i.e. the latest revision

Re: [galaxy-dev] RFC: Cheetah template in tool_dependencies.xml

2013-07-08 Thread Greg Von Kuster
Hello Björn, Thanks very much for the patch - I've committed alsightly alterered verson of it in changetset 10204:afec613d77fa which is currently running on the test tool shed. Greg Von Kuster On Jul 7, 2013, at 6:59 PM, Björn Grüning bjoern.gruen...@pharmazie.uni-freiburg.de wrote: Hi

[galaxy-dev] Setting up reference genomes for GATK Genotyper

2013-07-08 Thread Marco Ocana
Hi, I am running an instance of Galaxy that I setup using Cloudman. We are trying to use the GATK Unified Genotyper, but we are missing the reference genomes. I saw in your website instructions on how to setup reference genomes for some other tools, but not for the Unified Genotyper. Where can I

Re: [galaxy-dev] Error Uploading Directory of Files

2013-07-08 Thread Björn Grüning
Hi Nicholas, as pointed out by Peter and here: http://www.biostars.org/p/76021/ please check your white spaces and provide a little bit more information. Btw. what is wrong with biostar? Why the double post? Cheers, Bjoern Hi, Our lab recently installed a local version of Galaxy on a

Re: [galaxy-dev] ToolShed dependencies KeyError: 'invalid_repository_dependencies'

2013-07-08 Thread Greg Von Kuster
Hello Peter, Thanks for reporting this - I have committed a fix in changeset 10206:86efa5ac1fae, which is currently running on the test tool shed. Greg Von Kuster On Jul 8, 2013, at 11:35 AM, Peter Cock p.j.a.c...@googlemail.com wrote: Hi Greg, Previously my workflows had hard coded

[galaxy-dev] Connecting to new Galaxy Cloudman by FTP

2013-07-08 Thread Mohammad Heydarian
Hi, I am having trouble setting up a FTP connection with the recently released version of Galaxy Cloudman (ami-118bfc78). I have instantiated the new version of Galaxy Cloudman with CloudLaunchhttp://usegalaxy.org/cloudlaunch and also through the AWS EC2 wizard (using the same security group

Re: [galaxy-dev] gzipped fastq reader

2013-07-08 Thread Peter Cock
On Mon, Jul 8, 2013 at 10:24 PM, Robert Baertsch robert.baert...@gmail.com wrote: Peter and Dan, I like the idea of replacing all open() with galaxy_open() in all tools. You can tell the format by looking at the first 4 byes (see C code below from the UCSC browser team). Is there some pythonic

Re: [galaxy-dev] gzipped fastq reader

2013-07-08 Thread Peter Cock
On Mon, Jul 8, 2013 at 11:21 PM, Robert Baertsch rbaer...@ucsc.edu wrote: I respectfully disagree, If you want an extensible system, you should always wrap primitive system level calls. Any tools that opens a file that could be compressed would be affected. That is a huge number of tools. Do