[galaxy-dev] Galaxy can't find valid files in user folder

2012-07-02 Thread Ciara Ledero
Hi all,

My apologies for reposting this, but I thought I ought have asked this in a
separate thread instead of appending it to a previous message. Anyway, I
was able to set up a folder for a user (in my case, my own non-admin user
account). universe_wsgi.ini's been configured to allow users to utilize a
data library. I've also created a library for my non-admin user account.
When all was set, I tried uploading a file to the library. But Galaxy
prompted me that the folder did not contain any valid files. I thought I
provided the wrong path; I even thought I really did not put anything in
the folder. But when I checked, every thing seemed fine. I'm really
stumped. I've searched the mailing list and even the wiki, and
unfortunately I was not able to find a decent answer. I changed the file
extension to .fastq and I indicated that the file to be uploaded was a
FASTQ one, as Jen has suggested. I still got the same error in the end.
I've scanned the universe_wsgi.ini file to see if there's anything else
that I needed to configure, but I found none.

Thanks in advance for your help!

CL
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Re: [galaxy-dev] ValueError: Maximum key size is 448 bits

2012-07-02 Thread Sajoscha Sauer
Ok, found it! 
The 'id_secret' parameter in the universe_wsgi.ini was just too long. But I 
think something changed there, because we didn't change the id_secret, and it 
was working before. 

Anyway, now it's working! 

Cheers, 

Sajoscha

On Jun 27, 2012, at 4:09 PM, Sajoscha Sauer wrote:

 Hi, 
 
 I have updated our development installation of Galaxy. This wasn't done for a 
 long time, so it was a quite old version. 
 
 The update is done from galaxy-central. 
 
 I did hg pull, hg update and a db update. All that without errors. 
 
 But when I now try to start Galaxy, I get the following: 
 
 …
 Traceback (most recent call last):
   File /galaxy-dev/lib/galaxy/web/buildapp.py, line 82, in app_factory
 app = UniverseApplication( global_conf = global_conf, **kwargs )
   File /galaxy-dev/lib/galaxy/app.py, line 71, in __init__
 self.security = security.SecurityHelper( id_secret=self.config.id_secret )
   File /galaxy-dev/lib/galaxy/web/security/__init__.py, line 36, in __init__
 self.id_cipher = Blowfish.new( self.id_secret )
 ValueError: Maximum key size is 448 bits
 
 
 
 I'm totally lost on this one. Does anyone have an idea or a hint on where to 
 start? It would be highly appreciated! 
 
 Thanks a lot! 
 
 Sajoscha
 
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[galaxy-dev] error running workflow

2012-07-02 Thread J.W.F.van_der_Heijden


Hi all,

I have added some tools to my local install of Galaxy. To get to my final 
results I have to do a lot of steps.  When I try to run every step separately 
it turns out to work out fine. But when I try to run all steps at once with a 
workflow I get the following error message.

OperationalError: (OperationalError) database is locked u'UPDATE dataset SET 
update_time=?, state=? WHERE dataset.id = ?' ['2012-07-02 11:34:48.135419', 
'queued', 529]

I have noticed that the processes eat a lot of my computer resources. Other 
than that I have no clue what the problem could be.

Any tips are appreciated.

Kind regards,
Jaap van der Heijden
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Re: [galaxy-dev] error running workflow

2012-07-02 Thread Hans-Rudolf Hotz

Hi Jaap

are you using 'SQLite', if so, I recommend to switch to 'PostgreSQL', 
see: 
http://wiki.g2.bx.psu.edu/Admin/Config/Performance/Production%20Server/#Switching_to_a_database_server



Regards, Hans



On 07/02/2012 02:26 PM, j.w.f.van_der_heij...@lumc.nl wrote:



Hi all,

I have added some tools to my local install of Galaxy. To get to my
final results I have to do a lot of steps.  When I try to run every step
separately it turns out to work out fine. But when I try to run all
steps at once with a workflow I get the following error message.

|OperationalError: (OperationalError) database is locked u'UPDATE
dataset SET update_time=?, state=? WHERE dataset.id = ?' ['2012-07-02
11:34:48.135419', 'queued', 529]

I have noticed that the processes eat a lot of my computer resources.
Other than that I have no clue what the problem could be.

Any tips are appreciated.

Kind regards,
Jaap van der Heijden
|


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[galaxy-dev] Trackster : samtools not found error

2012-07-02 Thread Yec'han Laizet
Hello,

I try to use the visualization tool of my local instance of galaxy under 
ubuntu. Everytime I try to create a feature track from a sam file, I get the 
following error:
/bin/sh samtools not found

I have installed samtools from ubuntu reposotories and it works well as I can 
call it from the shell. Nevertheless, I still get the same error.
I checked that the samtools binary is in a folder included in my $PATH as 
described here:
http://dev.list.galaxyproject.org/Re-bin-sh-samtools-not-found-gt-gt-
WORKAROUND-td4585265.html
I tried to use an analyze data tool in galaxy interface that uses samtools 
and it worked.
It seems that it's only in trackster that samtools are not found.

Do you have an idea to fix it?

Best regards


Yec'han




Yec'han LAIZET
Ingénieur
Plateforme Genome Transcriptome
Tel: 05 57 12 27 75
_
INRA-UMR BIOGECO 1202
Equipe Genetique
69 route d'Arcachon
33612 CESTAS





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[galaxy-dev] problems installing mothur via toolshed

2012-07-02 Thread Hollander, Mattias de
Hi,

I am trying to install the mothur toolsuite via the toolshed but this 
returns in an HTTP 500 error:

URL: 
http://galaxy.nioo.knaw.nl/admin_toolshed/install_repository?tool_shed_url=http%3A%2F%2Ftoolshed.g2.bx.psu.edu%2Finstall_tool_dependencies=repo_info_dict=ea2e290f2209f91ca1303230f6223b698a53945f%3A7b226d6f746875725f746f6f6c7375697465223a205b224d6f74687572206d65746167656e6f6d69637320636f6d6d616e64732061732047616c61787920746f6f6c73222c2022687474703a2f2f746f6f6c736865642e67322e62782e7073752e6564752f7265706f732f6a6a6f686e736f6e2f6d6f746875725f746f6f6c7375697465222c2022323964623862363138346561222c20223130225d7dincludes_tools=True
File 
'/usr/local/lib/python2.7/dist-packages/Paste-1.7.5.1-py2.7.egg/paste/exceptions/errormiddleware.py',
 
line 144 in __call__
   app_iter = self.application(environ, sr_checker)
File 
'/usr/local/lib/python2.7/dist-packages/Paste-1.7.5.1-py2.7.egg/paste/debug/prints.py',
 
line 106 in __call__
   environ, self.app)
File 
'/usr/local/lib/python2.7/dist-packages/Paste-1.7.5.1-py2.7.egg/paste/wsgilib.py',
 
line 543 in intercept_output
   app_iter = application(environ, replacement_start_response)
File 
'/usr/local/lib/python2.7/dist-packages/Paste-1.7.5.1-py2.7.egg/paste/recursive.py',
 
line 84 in __call__
   return self.application(environ, start_response)
File 
'/usr/local/lib/python2.7/dist-packages/Paste-1.7.5.1-py2.7.egg/paste/httpexceptions.py',
 
line 633 in __call__
   return self.application(environ, start_response)
File 
'/home/galaxyTools/galaxy-central/lib/galaxy/web/framework/base.py', 
line 160 in __call__
   body = method( trans, **kwargs )
File 
'/home/galaxyTools/galaxy-central/lib/galaxy/web/framework/__init__.py', 
line 184 in decorator
   return func( self, trans, *args, **kwargs )
File 
'/home/galaxyTools/galaxy-central/lib/galaxy/web/controllers/admin_toolshed.py',
 
line 451 in install_repository
   clone_repository( repository_clone_url, os.path.abspath( 
relative_install_dir ), ctx_rev )
File '/home/galaxyTools/galaxy-central/lib/galaxy/util/shed_util.py', 
line 286 in clone_repository
   rev=[ ctx_rev ] )
File 
'/home/galaxyTools/galaxy-central/eggs/mercurial-2.1.2-py2.7-linux-x86_64-ucs4.egg/mercurial/commands.py',
 
line 1157 in clone
   branch=opts.get('branch'))
File 
'/home/galaxyTools/galaxy-central/eggs/mercurial-2.1.2-py2.7-linux-x86_64-ucs4.egg/mercurial/hg.py',
 
line 350 in clone
   destrepo.clone(srcrepo, heads=revs, stream=stream)
File 
'/home/galaxyTools/galaxy-central/eggs/mercurial-2.1.2-py2.7-linux-x86_64-ucs4.egg/mercurial/localrepo.py',
 
line 2280 in clone
   return self.pull(remote, heads)
File 
'/home/galaxyTools/galaxy-central/eggs/mercurial-2.1.2-py2.7-linux-x86_64-ucs4.egg/mercurial/localrepo.py',
 
line 1582 in pull
   remotephases = remote.listkeys('phases')
File 
'/home/galaxyTools/galaxy-central/eggs/mercurial-2.1.2-py2.7-linux-x86_64-ucs4.egg/mercurial/wireproto.py',
 
line 115 in plain
   encresref.set(self._submitone(f.func_name, encargsorres))
File 
'/home/galaxyTools/galaxy-central/eggs/mercurial-2.1.2-py2.7-linux-x86_64-ucs4.egg/mercurial/wireproto.py',
 
line 163 in _submitone
   return self._call(op, **args)
File 
'/home/galaxyTools/galaxy-central/eggs/mercurial-2.1.2-py2.7-linux-x86_64-ucs4.egg/mercurial/httprepo.py',
 
line 169 in _call
   fp = self._callstream(cmd, **args)
File 
'/home/galaxyTools/galaxy-central/eggs/mercurial-2.1.2-py2.7-linux-x86_64-ucs4.egg/mercurial/httprepo.py',
 
line 117 in _callstream
   resp = self.urlopener.open(req)
File '/usr/lib/python2.7/urllib2.py', line 397 in open
   response = meth(req, response)
File '/usr/lib/python2.7/urllib2.py', line 510 in http_response
   'http', request, response, code, msg, hdrs)
File '/usr/lib/python2.7/urllib2.py', line 435 in error
   return self._call_chain(*args)
File '/usr/lib/python2.7/urllib2.py', line 369 in _call_chain
   result = func(*args)
File '/usr/lib/python2.7/urllib2.py', line 518 in http_error_default
   raise HTTPError(req.get_full_url(), code, msg, hdrs, fp)
HTTPError: HTTP Error 500: Internal Server Error

Any ideas how to fix this?

Thanks!
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[galaxy-dev] GMOD Summer School application deadline

2012-07-02 Thread Scott Cain
Hello,

The deadline to apply for the GMOD Summer School is in one week, July
9th.  The application is available as a Google Form:

  
https://docs.google.com/spreadsheet/embeddedform?formkey=dG5hNGFiQ3UwYTV2LUZxZW04Qm1yZXc6MQ

In the GMOD Summer School (August 24-29, 2012) we will cover the
installation, configuration and use of a variety of GMOD tools,
including Chado, GBrowse, JBrowse and Galaxy.  For more information on
the course, see the course web page at

  http://gmod.org/wiki/2012_GMOD_Summer_School

The course will make heavy use of the Amazon Web Service (aka, the
Cloud) via a grant from Amazon.  Enrollment is limited to 24 students,
and the application process is competitive: the last few years we've
received over 75 applications for those 24 spots.

I look forward to seeing you in North Carolina in August!

-- 

Scott Cain, Ph. D.   scott at scottcain dot net
GMOD Coordinator (http://gmod.org/) 216-392-3087
Ontario Institute for Cancer Research
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Re: [galaxy-dev] problems installing mothur via toolshed

2012-07-02 Thread Greg Von Kuster
Hello Matthias,

I've just updated the tool shed to changeset revision 7332:e54f7684c919 and 
I've successfully installed the mothur toolsuite.   This revision includes a 
fix that may be related to the issue you encountered.  However, before you try 
reinstalling, can you reply with answers to each of the following?  This will 
help me know what steps you'll need to take before attempting the installation. 
 I'll reply back with what steps you should take as soon as I hear from you.

1. What changeset revision are you running on your Galaxy instance?

2. What is the setting for tool_path in your shed_tool_conf.xml file - is it 
the default of ../shed_tools ?

3. What changeset revision of the mothur tool suite were you trying to install 
- is the the tip of 5c77423823cb in the main tool shed?

Thanks!

Greg Von Kuster


On Jul 2, 2012, at 10:57 AM, Hollander, Mattias de wrote:

 Hi,
 
 I am trying to install the mothur toolsuite via the toolshed but this 
 returns in an HTTP 500 error:
 
 URL: 
 http://galaxy.nioo.knaw.nl/admin_toolshed/install_repository?tool_shed_url=http%3A%2F%2Ftoolshed.g2.bx.psu.edu%2Finstall_tool_dependencies=repo_info_dict=ea2e290f2209f91ca1303230f6223b698a53945f%3A7b226d6f746875725f746f6f6c7375697465223a205b224d6f74687572206d65746167656e6f6d69637320636f6d6d616e64732061732047616c61787920746f6f6c73222c2022687474703a2f2f746f6f6c736865642e67322e62782e7073752e6564752f7265706f732f6a6a6f686e736f6e2f6d6f746875725f746f6f6c7375697465222c2022323964623862363138346561222c20223130225d7dincludes_tools=True
 File 
 '/usr/local/lib/python2.7/dist-packages/Paste-1.7.5.1-py2.7.egg/paste/exceptions/errormiddleware.py',
  
 line 144 in __call__
   app_iter = self.application(environ, sr_checker)
 File 
 '/usr/local/lib/python2.7/dist-packages/Paste-1.7.5.1-py2.7.egg/paste/debug/prints.py',
  
 line 106 in __call__
   environ, self.app)
 File 
 '/usr/local/lib/python2.7/dist-packages/Paste-1.7.5.1-py2.7.egg/paste/wsgilib.py',
  
 line 543 in intercept_output
   app_iter = application(environ, replacement_start_response)
 File 
 '/usr/local/lib/python2.7/dist-packages/Paste-1.7.5.1-py2.7.egg/paste/recursive.py',
  
 line 84 in __call__
   return self.application(environ, start_response)
 File 
 '/usr/local/lib/python2.7/dist-packages/Paste-1.7.5.1-py2.7.egg/paste/httpexceptions.py',
  
 line 633 in __call__
   return self.application(environ, start_response)
 File 
 '/home/galaxyTools/galaxy-central/lib/galaxy/web/framework/base.py', 
 line 160 in __call__
   body = method( trans, **kwargs )
 File 
 '/home/galaxyTools/galaxy-central/lib/galaxy/web/framework/__init__.py', 
 line 184 in decorator
   return func( self, trans, *args, **kwargs )
 File 
 '/home/galaxyTools/galaxy-central/lib/galaxy/web/controllers/admin_toolshed.py',
  
 line 451 in install_repository
   clone_repository( repository_clone_url, os.path.abspath( 
 relative_install_dir ), ctx_rev )
 File '/home/galaxyTools/galaxy-central/lib/galaxy/util/shed_util.py', 
 line 286 in clone_repository
   rev=[ ctx_rev ] )
 File 
 '/home/galaxyTools/galaxy-central/eggs/mercurial-2.1.2-py2.7-linux-x86_64-ucs4.egg/mercurial/commands.py',
  
 line 1157 in clone
   branch=opts.get('branch'))
 File 
 '/home/galaxyTools/galaxy-central/eggs/mercurial-2.1.2-py2.7-linux-x86_64-ucs4.egg/mercurial/hg.py',
  
 line 350 in clone
   destrepo.clone(srcrepo, heads=revs, stream=stream)
 File 
 '/home/galaxyTools/galaxy-central/eggs/mercurial-2.1.2-py2.7-linux-x86_64-ucs4.egg/mercurial/localrepo.py',
  
 line 2280 in clone
   return self.pull(remote, heads)
 File 
 '/home/galaxyTools/galaxy-central/eggs/mercurial-2.1.2-py2.7-linux-x86_64-ucs4.egg/mercurial/localrepo.py',
  
 line 1582 in pull
   remotephases = remote.listkeys('phases')
 File 
 '/home/galaxyTools/galaxy-central/eggs/mercurial-2.1.2-py2.7-linux-x86_64-ucs4.egg/mercurial/wireproto.py',
  
 line 115 in plain
   encresref.set(self._submitone(f.func_name, encargsorres))
 File 
 '/home/galaxyTools/galaxy-central/eggs/mercurial-2.1.2-py2.7-linux-x86_64-ucs4.egg/mercurial/wireproto.py',
  
 line 163 in _submitone
   return self._call(op, **args)
 File 
 '/home/galaxyTools/galaxy-central/eggs/mercurial-2.1.2-py2.7-linux-x86_64-ucs4.egg/mercurial/httprepo.py',
  
 line 169 in _call
   fp = self._callstream(cmd, **args)
 File 
 '/home/galaxyTools/galaxy-central/eggs/mercurial-2.1.2-py2.7-linux-x86_64-ucs4.egg/mercurial/httprepo.py',
  
 line 117 in _callstream
   resp = self.urlopener.open(req)
 File '/usr/lib/python2.7/urllib2.py', line 397 in open
   response = meth(req, response)
 File '/usr/lib/python2.7/urllib2.py', line 510 in http_response
   'http', request, response, code, msg, hdrs)
 File '/usr/lib/python2.7/urllib2.py', line 435 in error
   return self._call_chain(*args)
 File '/usr/lib/python2.7/urllib2.py', line 369 in _call_chain
   result = func(*args)
 File '/usr/lib/python2.7/urllib2.py', line 518 in http_error_default
   raise HTTPError(req.get_full_url(), code, msg, hdrs, fp)
 HTTPError: HTTP 

[galaxy-dev] sanitizer for carriage return

2012-07-02 Thread Jennifer Jackson

___

On 7/1/12 3:05 AM, Katrien Bernaerts wrote:

Dear,
I am making a Galaxy appliciation with a text area. In the text area, 
the user can copy/paste sequences. However, all carriage returns (e.g. 
after the comment line) are converted to XX by Galaxy. I found that a 
sanitizer can be used for specal characters, but I could not figure 
out how to configure the sanitizer for a carriage return. Does anyone 
have an idea how to handle carriage returns in the user input?

Thanks in advance,
--
Katrien Bernaerts




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Re: [galaxy-dev] FastQC Tool Errors

2012-07-02 Thread Josh Nielsen
Sure enough, this fixed the problem. I just installed the latest Sun JRE
and it is working now. Thanks for the suggestion! I would have never
guessed.

-Josh

On Mon, Jun 25, 2012 at 11:34 AM, simon andrews 
simon.andr...@babraham.ac.uk wrote:

  Yes, that's the broken version of gcj.  I don't have a Centos machine
 here at the moment, but I think if you install OpenJDK and use the
 alternatives system to select that as the default JRE then that should fix
 things.

  Simon.

  On 25 Jun 2012, at 17:19, Josh Nielsen wrote:

  Hi Simon,

 I recently installed Java with the yum package manager on our compute
 nodes, and our cluster is a Centos 6 environment. Here is what the results
 of java -version returned on the compute nodes:

 *bash# java -version*
 *java version 1.5.0*
 *gij (GNU libgcj) version 4.4.4 20100726 (Red Hat 4.4.4-13)*
 *
 *
 *Copyright (C) 2007 Free Software Foundation, Inc.*
 *This is free software; see the source for copying conditions.  There is
 NO*
 *warranty; not even for MERCHANTABILITY or FITNESS FOR A PARTICULAR
 PURPOSE*.

  Is this version of Java too old? Perhaps I need to install the JRE
 manually?

 Thanks!

 On *Sat Jun 23 04:20:53 EDT 2012*, Simon Andrews 
 simon.andr...@babraham.ac.uk 
 galaxy-dev%40lists.bx.psu.edu?Subject=Re%3A%20%5Bgalaxy-dev%5D%20FastQC%20Tool%20ErrorsIn-Reply-To=%3CD9909700-8628-4478-814C-449803EE45F1%40babraham.ac.uk%3E
  wrote:

 Are you by any chance running an older version of gcj as your java version?  
 There is a known bug in some of these where they don't correctly configure 
 the headless environment, even if the correct parameters are passed.  This 
 causes exactly the kind of errors you're seeing.

 If this is the case you'll need to install a more recent JRE (or update your 
 path to point to one which is already present).

 Simon.


 On Sat, Jun 23, 2012 at 6:30 AM, Josh Nielsen jniel...@hudsonalpha.com
 wrote:
  Hello,
 
  I am having an issue with getting the FastQC tool to work with Galaxy
 on our
  server. I downloaded the FastQC files (version 0.8.0) and changed the
  directory that the wrapper script looks for the 'fastqc' executable in,
 but
  when we run a job with it we have been getting the following output:
 
  Started analysis of Clip
 
  Approx 5% complete for Clip
  Approx 10% complete for Clip
  ...
  ...
  Approx 95% complete for Clip
  Approx 100% complete for Clip
 
  Analysis complete for Clip
 
  (.:9754): Gtk-WARNING **: cannot open display: 
 
  And then the job shows as failed in Galaxy. The output .dat file just
 has
  that same output/error message in it (though it seems to indicate it
 got to
  100%). Also when I try to execute the fastqc file directly (albeit with
 no
  arguments) I get this:
 
  Exception in thread main java.awt.HeadlessException:
  No X11 DISPLAY variable was set, but this program performed an operation
  which requires it.
  at
  java.awt.GraphicsEnvironment.checkHeadless(GraphicsEnvironment.java:173)
  at java.awt.Window.init(Window.java:437)
  at java.awt.Frame.init(Frame.java:419)
  at java.awt.Frame.init(Frame.java:384)
  at javax.swing.JFrame.init(JFrame.java:174)
  at
 
 uk.ac.bbsrc.babraham.FastQC.FastQCApplication.init(FastQCApplication.java:271)
  at
 
 uk.ac.bbsrc.babraham.FastQC.FastQCApplication.main(FastQCApplication.java:102)
 
  Both errors seem to have something to do with the graphical GUI
 component of
  FastQC (which I have seen some screenshots for on the FastQC webpage).
 If
  this application is GUI-driven how did the online PSU Galaxy get it to
 work
  with their wrapper script when the tools are run in a command-line
  environment with no X11 or Gtk? Essentially I'm just wondering what
 steps
  I'm missing here to getting this to work with our Galaxy mirror, other
 than
  just dropping the executable in place? Any suggestions?
 
  Thanks,
  Josh
 
 
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 --
 Ross Lazarus MBBS MPH;
 Associate Professor, Harvard Medical School;
 Head, Medical Bioinformatics, BakerIDI; Tel: +61 385321444;



  The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT 
 *Registered
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Re: [galaxy-dev] Trackster : samtools not found error

2012-07-02 Thread Jeremy Goecks
Hello,

It's likely that samtools is not in Galaxy's path because Trackster uses the 
same path as Galaxy does. Also, many tools in Galaxy use the Galaxy PySam egg 
rather than the samtools binary, so it's possible that you can run a tool that 
uses samtools without the binary in Galaxy's path. Which tool were you able 
to run successfully?

Probably the best idea is to simply convert SAM to BAM and then uses Trackster 
on the BAM. Visualizing SAM datasets is occasionally problematic due to missing 
metadata and large sizes, and SAM is simply converted to BAM before 
visualization anyways.

Best,
J. 

On Jul 2, 2012, at 9:37 AM, Yec'han Laizet wrote:

 Hello,
 
 I try to use the visualization tool of my local instance of galaxy under 
 ubuntu. Everytime I try to create a feature track from a sam file, I get the 
 following error:
 /bin/sh samtools not found
 
 I have installed samtools from ubuntu reposotories and it works well as I can 
 call it from the shell. Nevertheless, I still get the same error.
 I checked that the samtools binary is in a folder included in my $PATH as 
 described here:
 http://dev.list.galaxyproject.org/Re-bin-sh-samtools-not-found-gt-gt-
 WORKAROUND-td4585265.html
 I tried to use an analyze data tool in galaxy interface that uses samtools 
 and it worked.
 It seems that it's only in trackster that samtools are not found.
 
 Do you have an idea to fix it?
 
 Best regards
 
 
 Yec'han
 
 
 
 
 Yec'han LAIZET
 Ingénieur
 Plateforme Genome Transcriptome
 Tel: 05 57 12 27 75
 _
 INRA-UMR BIOGECO 1202
 Equipe Genetique
 69 route d'Arcachon
 33612 CESTAS
 
 
 
 
 
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