Re: [galaxy-dev] Galaxy and Toolshed issues

2012-08-01 Thread SHAUN WEBB


Hi Greg, thanks for the response

Quoting Greg Von Kuster g...@bx.psu.edu on Tue, 31 Jul 2012 11:50:36 -0400:


Hi Shaun,

On Jul 31, 2012, at 9:37 AM, SHAUN WEBB wrote:



Hi, I have been trying out the latest version of Galaxy and a newly  
installed local toolshed and I have a few questions/suggestions/bug  
reports:


1. Tool Versioning:

a) As I understand from the wiki, when I create a new version of a  
tool in a tool shed I must then install the latest version in my  
Galaxy instance.


This is not correct - you can choose to install any valid version of  
the tool(s).  Can you let me know what content of the wiki resulted  
in this confusion?  I'll be sure to make it more clear.




This then shows up as two separate tools in my tool menu.  
Presumably it is best to change the xml description tag to reflect  
the version number and make tool versions distinguishable in the  
Galaxy tool menu?


b) Would it make more sense to have a single entry for each tool in  
the tool menu and a drop down to choose which version to run in the  
tool gui? Similar to when I try to rerun a tool that is no longer  
installed I get a drop down option to use a derivative of that tool  
if it exists.


Your above recommendation in (b) is exactly what will occur as soon  
as I get a chance to implement it.




Perhaps my wording was unclear here. What I meant was if a new version  
of a tool is created in a tool shed I have to install that version to  
be able to use it, rather than using 'get updates'. This then results  
in multiple listings of the tool. But it sounds like you plan on  
improving this process anyway.








c) The toolshed interface allows me to change the name of my tool.  
If I do this, then go to my Galaxy admin ui and try to get updates  
for my tool then it fails. Does it make sense to change a tools  
name after it is created? Is it possible to track previous tool  
names to avoid this happening?


This should not occur.  Where are you able to change the name of  
your tool?  You are not even allowed to change the name of the  
repository if it has been cloned (you can change the name of the  
repository as long as it has not been cloned).  Can you send me a  
screen shot of hte page that allows you to change the name of your  
tool in the tool shed?  I'll provide a fix as soon as I see where  
the issue lies.


OK, I guess I changed the name of my repository not tool. I then  
installed the tool via my local Galaxy instance. I can still change  
the name of the repository in my toolshed and when I try to get  
updates via Galaxy admin panel I get the following server error:  
AttributeError: 'NoneType' object has no attribute 'repo_path'







Running toolshed tools:

I have no problems installing tools from toolsheds via Galaxy admin  
interface, however I can't get these tools to run. They stay Grey  
forever and look like this in the manage jobs table:


65	x...@.xx.ac.uk	0 minutes  
ago	toolshed.g2.bx.psu.edu/repos/aaronquinlan/bedtools/bedtools_coveragebed_counts/0.1.0	new	None	None	None


Do I have to set a tool runner or some other option for shed_tools?



No special configuration settings are required in your local Galaxy  
instance to to run tools installed from a tool shed.  If they stay  
in the queued state, there must be an issue in your local Galaxy  
instance that causes installed tools as well as tools included in  
the distribution to remain queued.  It has nothing to do with the  
fact they were installed.




No. All other tools run fine. Tools installed from either my toolshed  
or the Galaxy toolshed remain queued. If this is not a known issue  
then I will try to dig a little deeper to find the problem. Any  
pointers would be useful if you can think of why there should be a  
difference.



Thanks for your help!
Shaun

--
The University of Edinburgh is a charitable body, registered in
Scotland, with registration number SC005336.


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Re: [galaxy-dev] Upload local files

2012-08-01 Thread Sascha Kastens
I finally wrote a script which can be used as a separate tool. The script takes 
one or more file paths which

can be either a file or a directory.

As soon as the script does not know the exact number of files to upload it 
places links to the files in

__new_file_path__. The problem now is, that Galaxy copies the files pointed to 
into it?s database directory

which results in the former problem of space overhead. Is there a way to let 
Galaxy only copy the links?

 

Thanks in advance,

 

Sascha Kastens

Project Manager

 

GATC Biotech AG

Jakob-Stadler-Platz 7

D-78467 Konstanz

 

Phone: +49 (0) 7531-81604110

Fax: +49 (0) 7531-816081

Email:  s.kast...@gatc-biotech.com

 

http://www.gatc-biotech.com

http://www.twitter.com/gatcbiotech

http://www.facebook.com/gatcbiotech

http://www.xing.com/companies/gatcbiotechag

 

GATC Biotech AG
Chairman Supervisory Board: Fritz Pohl
Board of Directors: Peter Pohl, Thomas Pohl, Dr. Marcus Benz
UID: DE 142 315 733 | Registration: Konstanz, HRB 1757 | Registered Office: 
Konstanz

 

 

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Original Message

processed by CONSOLIDATE

Subject:

Re: [galaxy-dev] Upload local files

Sent:

Dienstag, 31. Juli 2012 00:08

From:

Smithies, Russell (russell.smith...@agresearch.co.nz)


Yes, we d like to do this as well.

Our users data are all on the servers, not on their Windows PCs and requiring 
the admin create shared data libraries is an overhead we d rather avoid.

 

Thanx,

 

Russell Smithies
Infrastructure Technician

T 03 489 9085
M 027 4734 600
E russell.smith...@agresearch.co.nz

Invermay Agricultural Centre
Puddle Alley, Private Bag 50034, Mosgiel 9053, New Zealand

T  +64 3 489 3809  F  +64 3 489 3739  www.agresearch.co.nz

 

 

 

 

From: galaxy-dev-boun...@lists.bx.psu.edu 
[mailto:galaxy-dev-boun...@lists.bx.psu.edu] On Behalf Of Sascha Kastens
Sent: Tuesday, 31 July 2012 2:09 a.m.
To: galaxy-dev@lists.bx.psu.edu
Subject: [galaxy-dev] Upload local files

 

Hi devs,

 

when using the upload tool it would be nice to let the user

specify paths to files stored on our internal servers like the admin

can when choosing to add data to a library. Also it would be nice

to be able to link to these files.

 

Unfortunately I was not able to find the tool for uploading

files into a library so it would be nice if you can give me a hint.

With this information I should be able to extend the normal upload

tool.

 

Thanks in advance!

 

Cheers,

Sascha Kastens

Project Manager

 

GATC Biotech AG

Jakob-Stadler-Platz 7

D-78467 Konstanz

 

Phone: +49 (0) 7531-81604110

Fax: +49 (0) 7531-816081

Email:  s.kast...@gatc-biotech.com

 

http://www.gatc-biotech.com

http://www.twitter.com/gatcbiotech

http://www.facebook.com/gatcbiotech

http://www.xing.com/companies/gatcbiotechag

 

GATC Biotech AG
Chairman Supervisory Board: Fritz Pohl
Board of Directors: Peter Pohl, Thomas Pohl, Dr. Marcus Benz
UID: DE 142 315 733 | Registration: Konstanz, HRB 1757 | Registered Office: 
Konstanz

 

 

The information contained in this email is intended solely for the addressee. 
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Re: [galaxy-dev] Update Galaxy instance with Mercurial error

2012-08-01 Thread Dannon Baker
Hi Makis,

This should work to get you an update-able galaxy instance going forward, 
without having to manually migrate anything.

Please note that this method will clobber any existing changes to code, so if 
you've modified any of the core galaxy you'll need to patch the new files.  All 
of your data and database settings will remain intact (but still do steps 1 and 
2).

1)  Back up everything, just in case
2)  Make sure you did step #1
2)  cd into the root galaxy directory and `hg init`
3)  create a file, galaxy_root/.hg/hgrc with the following two lines:

[paths]
default = https://bitbucket.org/galaxy/galaxy-dist

4)  And again from the galaxy root `hg pull`
5)  Finally `hg update -C`

And you should be good to go.

-Dannon


On Jul 31, 2012, at 6:05 AM, Makis Ladoukakis makis4e...@hotmail.com wrote:

 Dear Galaxy users,
 
 I am running a Galaxy instance in my institution's server and I want to keep 
 it up to date with the latest release. Nevertheless when i try hg incomingi 
 get the following error:
 
 abort: There is no Mercurial repository here (.hg not found)!
 
 Most probably the administrator of the server has downloaded the Galaxy-dist 
 directory without the use of Mercurial (I was suspicious of that all along as 
 the directory of the Galaxy installation is named galaxy_dist and not 
 galaxy-dist). Is there another way to update my instance?
 
 Thank you,
 Makis Ladoukakis 
 
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Re: [galaxy-dev] egg distribution error when running galaxy-central

2012-08-01 Thread Scott McManus

Clare-

Have you resolved this yet? I had seen similar issues with respect
to pycrypto before I used virtualenv, but I don't believe that I saw
it afterwards. I'll take a look and see if I can reproduce it.

I only have obvious things to recommend right now. For example,
make sure that the environment has been loaded each time.
Check 
http://wiki.g2.bx.psu.edu/Admin/Config/Performance/Production%20Server#Use_a_clean_environment
 ,
which I'm sure you've already done. 

-Scott

- Original Message -
 On 1 August 2012 15:28, Clare Sloggett s...@unimelb.edu.au wrote:
 I can run
  galaxy-dist without problems and have been working with that (so
  its
  eggs are all installed already), but now I want to create a pull
  request so want to run galaxy-dist.
 
 oops, of course I mean 'so want to run galaxy-central.'
 
 
 
 
 --
 
 Clare Sloggett
 Research Fellow / Bioinformatician
 Life Sciences Computation Centre
 Victorian Life Sciences Computation Initiative
 University of Melbourne, Parkville Campus
 187 Grattan Street, Carlton, Melbourne
 Victoria 3010, Australia
 Ph: 03 903 53357  M: 0414 854 759
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Re: [galaxy-dev] Local instance and storage space to provide

2012-08-01 Thread Scott McManus

Hi Sarah-

I'm sure others have experiences that could help, so I hope they will
chime in. Nate put together some slides two years ago on production setups,
including some architecture for the server main.g2.bx.psu.edu:
http://wiki.g2.bx.psu.edu/Events/GDC2010?action=AttachFiledo=gettarget=GDC2010_building_scalable.pdf

There is also a wiki page describing production environments:
http://wiki.g2.bx.psu.edu/Admin/Config/Performance/Production%20Server

Again, I'm sure others have had different experiences with both more and less
hardware on hand. Of course your mileage may vary based on the tools being used
(which in your case I'm guessing is Tophat), the number of datasets that will 
need to be analyzed, and so on.

I hope that helps!

-Scott

- Original Message -
 Dear all,
 
 To purchase equipment related to a local instance of Galaxy, I would
 like to have your feedback.
 
 Did you notice load problems when 10 to 20 users launch jobs at the
 same
 time and on a single instance of Galaxy, with mi-RNA or RNA seq data
 ?
 
 What do you recommend in terms of storage space, number of nodes,
 number
 of instances?
 
 Thank you in advance,
 Sarah Maman
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Re: [galaxy-dev] PBS Server Throwing Errors

2012-08-01 Thread Scott McManus

Hi Dan-

Thanks again for your great hospitality at UCI! 

It looks like you've done most of the sane things, and a quick
check looks like the PBS egg is requesting protocol version 1
while the server supports protocol version 2. One possibility
is that there is a protocol mismatch, which could be the result
of using an old library. We can check if the egg is at fault
first - try the following link for compiling the pbs_python.py
egg yourself and try the example to see if the connection and
a simple stats-gathering operation (the examples/pbsnodes-a.py
script from pbs_python) works:
https://subtrac.sara.nl/oss/pbs_python/wiki/TorqueInstallation

-Scott

- Original Message -
 Hi, Galaxy Community,
 
 Greetings from The University of Chicago; I hope all who attended the
 Galaxy conference enjoyed it as much as I did.  I have searched the
 mailing list archives as well as Google to a resolve problem I am
 seeing, however I am somewhat at a loss as to the next course of
 action I should be taking to bring this issue to a close.  I am
 hoping
 that one of the bright minds on this mailing list could help me  shed
 some light on the solution to my problem, or at least help me
 identify
 a root cause.  I have configured Galaxy to integrate with TORQUE
 (version 4.0.2) server, and successfully built the PBS python egg as
 specified in the Galaxy documentation.  I am using Python version 2.6
 and the latest build of Galaxy.  Whenever I launch a job from the
 Galaxy UI, I get the following error message(s) on the PBS server:
 
 07/30/2012 15:42:48;0080;PBS_Server;Req;dis_request_read;conflicting
 version numbers, 1 detected, 2 expected
 07/30/2012 15:42:48;0080;PBS_Server;Req;req_reject;Reject reply
 code=15058(Bad DIS based Request Protocol MSG=cannot decode message),
 aux=0, type=AlternateUserAuthentication, from galaxy@
 07/30/2012 15:42:48;0080;PBS_Server;Req;dis_request_read;conflicting
 version numbers, 1 detected, 2 expected
 07/30/2012 15:42:48;0080;PBS_Server;Req;req_reject;Reject reply
 code=15058(Bad DIS based Request Protocol MSG=cannot decode message),
 aux=0, type=QueueJob, from galaxy@
 07/30/2012 15:42:48;0080;PBS_Server;Req;dis_request_read;conflicting
 version numbers, 1 detected, 2 expected
 07/30/2012 15:42:48;0080;PBS_Server;Req;req_reject;Reject reply
 code=15058(Bad DIS based Request Protocol MSG=cannot decode message),
 aux=0, type=Disconnect, from galaxy@
 07/30/2012 15:43:01;0002;PBS_Server;Svr;PBS_Server;Torque Server
 Version = 4.0.2, loglevel = 1
 
 One thing I did notice, that suggests there might be a problem, is
 that there is no hostname after the galaxy@; most of the other
 messages in this log file have a host name appended to log entry,
 i.e.:
 
 07/30/2012 15:43:38;0100;PBS_Server;Req;;Type StatusJob request
 received from root@sc01, sock=10
 
 I have completed a tcpdump on the schedule node, and I can definitely
 see bi-directional traffic between the Galaxy server and the
 scheduler
 node on TCP port 15001.  In addition to this, I have installed the
 TORQUE client tools on on the Galaxy server, and can spawn an
 interactive job with qsub -I, as well as check the status of queued
 jobs using qstat (from the Galaxy server).  This suggests to me that
 there a potential problem with the PBS Egg, although I am not
 certain.
  Has anybody seen something like this before, or could somebody point
 me in the right direction?  We do have a support contract with
 Adaptive Computing, and I am opening a ticket with them as well,
 however I wanted to reach out to the Galaxy community to cover all of
 my bases.  Thank-you so much for taking the time to read my email.
 
 Dan Sullivan
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Re: [galaxy-dev] PBS Server Throwing Errors

2012-08-01 Thread Scott McManus

I've been told by Galaxy folks to keep this on galaxy-dev.
My apologies for any confusion.

-Scott

- Original Message -
 
 Hi Dan-
 
 Thanks again for your great hospitality at UCI!
 
 It looks like you've done most of the sane things, and a quick
 check looks like the PBS egg is requesting protocol version 1
 while the server supports protocol version 2. One possibility
 is that there is a protocol mismatch, which could be the result
 of using an old library. We can check if the egg is at fault
 first - try the following link for compiling the pbs_python.py
 egg yourself and try the example to see if the connection and
 a simple stats-gathering operation (the examples/pbsnodes-a.py
 script from pbs_python) works:
 https://subtrac.sara.nl/oss/pbs_python/wiki/TorqueInstallation
 
 -Scott
 
 - Original Message -
  Hi, Galaxy Community,
  
  Greetings from The University of Chicago; I hope all who attended
  the
  Galaxy conference enjoyed it as much as I did.  I have searched the
  mailing list archives as well as Google to a resolve problem I am
  seeing, however I am somewhat at a loss as to the next course of
  action I should be taking to bring this issue to a close.  I am
  hoping
  that one of the bright minds on this mailing list could help me
   shed
  some light on the solution to my problem, or at least help me
  identify
  a root cause.  I have configured Galaxy to integrate with TORQUE
  (version 4.0.2) server, and successfully built the PBS python egg
  as
  specified in the Galaxy documentation.  I am using Python version
  2.6
  and the latest build of Galaxy.  Whenever I launch a job from the
  Galaxy UI, I get the following error message(s) on the PBS server:
  
  07/30/2012
  15:42:48;0080;PBS_Server;Req;dis_request_read;conflicting
  version numbers, 1 detected, 2 expected
  07/30/2012 15:42:48;0080;PBS_Server;Req;req_reject;Reject reply
  code=15058(Bad DIS based Request Protocol MSG=cannot decode
  message),
  aux=0, type=AlternateUserAuthentication, from galaxy@
  07/30/2012
  15:42:48;0080;PBS_Server;Req;dis_request_read;conflicting
  version numbers, 1 detected, 2 expected
  07/30/2012 15:42:48;0080;PBS_Server;Req;req_reject;Reject reply
  code=15058(Bad DIS based Request Protocol MSG=cannot decode
  message),
  aux=0, type=QueueJob, from galaxy@
  07/30/2012
  15:42:48;0080;PBS_Server;Req;dis_request_read;conflicting
  version numbers, 1 detected, 2 expected
  07/30/2012 15:42:48;0080;PBS_Server;Req;req_reject;Reject reply
  code=15058(Bad DIS based Request Protocol MSG=cannot decode
  message),
  aux=0, type=Disconnect, from galaxy@
  07/30/2012 15:43:01;0002;PBS_Server;Svr;PBS_Server;Torque Server
  Version = 4.0.2, loglevel = 1
  
  One thing I did notice, that suggests there might be a problem, is
  that there is no hostname after the galaxy@; most of the other
  messages in this log file have a host name appended to log entry,
  i.e.:
  
  07/30/2012 15:43:38;0100;PBS_Server;Req;;Type StatusJob request
  received from root@sc01, sock=10
  
  I have completed a tcpdump on the schedule node, and I can
  definitely
  see bi-directional traffic between the Galaxy server and the
  scheduler
  node on TCP port 15001.  In addition to this, I have installed the
  TORQUE client tools on on the Galaxy server, and can spawn an
  interactive job with qsub -I, as well as check the status of queued
  jobs using qstat (from the Galaxy server).  This suggests to me
  that
  there a potential problem with the PBS Egg, although I am not
  certain.
   Has anybody seen something like this before, or could somebody
   point
  me in the right direction?  We do have a support contract with
  Adaptive Computing, and I am opening a ticket with them as well,
  however I wanted to reach out to the Galaxy community to cover all
  of
  my bases.  Thank-you so much for taking the time to read my email.
  
  Dan Sullivan
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Re: [galaxy-dev] egg distribution error when running galaxy-central

2012-08-01 Thread Scott McManus

I haven't been able to reproduce this yet with the instructions you
gave, but I'm not using the same environment. Can you give me an idea 
of what tools you're using outside of SciPy/NumPy/Enthought stuff?

There is the possibility that the virtualenv.py script isn't being
sourced correctly. We can check if it's actually using the correct
environment by calling echo $PATH and checking that the path is
pointing to the virtual environment. For example, I installed 
virtualenv stuff under /home/smcmanus/clare/galaxy_env/bin, and
I got:
(galaxy_env)$ echo $PATH
/home/smcmanus/clare/galaxy_env/bin:/usr/local/bin:other stuff deleted

-Scott

- Original Message -
 Hi all,
 
 I'm trying to run galaxy-central on my laptop in order to play around
 with some changes, and I'm having trouble getting it to run. I can
 run
 galaxy-dist without problems and have been working with that (so its
 eggs are all installed already), but now I want to create a pull
 request so want to run galaxy-dist. I'm not trying to install any
 extra tools or data, just the code.
 
 I'm running on OSX 10.7.4 and using virtualenv. I have Enthought
 installed, and I assume I will be using its version of python by
 default. The default python seems to be 2.7.3.
 
 I'm using the same virtualenv environment for galaxy-dist and
 galaxy-central (though it doesn't seem to matter if I give
 galaxy-central its own environment, I see the same error). So the
 steps were:
 - create a virtualenv environment and activate it
 - get galaxy-dist and call run.sh - it asked me to build quite a lot
 of dependencies myself, which was just a matter of running the
 requested commands, and then it worked with no problems.
 - shut down galaxy-dist, and in another directory, get galaxy-central
 and call run.sh. I think it asked me to build a couple of
 dependencies, but then it gives up with the following:
 
 (galaxy_env)Clares-MacBook-Pro:galaxy-central clare$ sh run.sh
 --reload
 Some eggs are out of date, attempting to fetch...
 Warning: MarkupSafe (a dependent egg of Mako) cannot be fetched
 Warning: pycrypto (a dependent egg of Fabric) cannot be fetched
 Warning: simplejson (a dependent egg of WebHelpers) cannot be fetched
 Fetched http://eggs.g2.bx.psu.edu/ssh/ssh-1.7.14-py2.7.egg
 One of Galaxy's managed eggs depends on something which is missing,
 this is almost certainly a bug in the egg distribution.
 Dependency ssh requires pycrypto=2.1,!=2.4
 Traceback (most recent call last):
   File ./scripts/fetch_eggs.py, line 30, in module
 c.resolve() # Only fetch eggs required by the config
   File
   /Users/clare/galaxy/galaxy-central/lib/galaxy/eggs/__init__.py,
 line 345, in resolve
 egg.resolve()
   File
   /Users/clare/galaxy/galaxy-central/lib/galaxy/eggs/__init__.py,
 line 168, in resolve
 dists = pkg_resources.working_set.resolve( (
 self.distribution.as_requirement(), ), env, self.fetch )
   File
   
 /Users/clare/galaxy/galaxy_env/lib/python2.7/site-packages/setuptools-0.6c11-py2.7.egg/pkg_resources.py,
 line 569, in resolve
 raise VersionConflict(dist,req) # XXX put more info here
 pkg_resources.VersionConflict: (ssh 1.7.14
 (/Users/clare/galaxy/galaxy-central/eggs/ssh-1.7.14-py2.7.egg),
 Requirement.parse('pycrypto=2.1,!=2.4'))
 Fetch failed.
 
 Any idea what is causing this?
 
 Thanks,
 Clare
 
 --
 
 Clare Sloggett
 Research Fellow / Bioinformatician
 Life Sciences Computation Centre
 Victorian Life Sciences Computation Initiative
 University of Melbourne, Parkville Campus
 187 Grattan Street, Carlton, Melbourne
 Victoria 3010, Australia
 Ph: 03 903 53357  M: 0414 854 759
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Re: [galaxy-dev] [galaxy-user] Fwd: Local instance and storage space to provide

2012-08-01 Thread Scott McManus

Sorry - I fouled up and should have kept this on galaxy-dev.

-Scott

- Original Message -
 
 I'm forwarding this to galaxy-user instead of galaxy-dev.
 
 -Scott
 
 - Forwarded Message -
 From: Scott McManus scottmcma...@gatech.edu
 To: Sarah Maman sarah.ma...@toulouse.inra.fr
 Cc: galaxy-dev@lists.bx.psu.edu
 Sent: Wednesday, August 1, 2012 4:18:29 PM
 Subject: Re: [galaxy-dev] Local instance and storage space to provide
 
 
 Hi Sarah-
 
 I'm sure others have experiences that could help, so I hope they will
 chime in. Nate put together some slides two years ago on production
 setups,
 including some architecture for the server main.g2.bx.psu.edu:
 http://wiki.g2.bx.psu.edu/Events/GDC2010?action=AttachFiledo=gettarget=GDC2010_building_scalable.pdf
 
 There is also a wiki page describing production environments:
 http://wiki.g2.bx.psu.edu/Admin/Config/Performance/Production%20Server
 
 Again, I'm sure others have had different experiences with both more
 and less
 hardware on hand. Of course your mileage may vary based on the tools
 being used
 (which in your case I'm guessing is Tophat), the number of datasets
 that will
 need to be analyzed, and so on.
 
 I hope that helps!
 
 -Scott
 
 - Original Message -
  Dear all,
  
  To purchase equipment related to a local instance of Galaxy, I
  would
  like to have your feedback.
  
  Did you notice load problems when 10 to 20 users launch jobs at the
  same
  time and on a single instance of Galaxy, with mi-RNA or RNA seq
  data
  ?
  
  What do you recommend in terms of storage space, number of nodes,
  number
  of instances?
  
  Thank you in advance,
  Sarah Maman
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