[galaxy-dev] possible to resume failed workflow?

2012-05-24 Thread J. Greenbaum

Hi, 

I've created a few workflows and have been having issues with some steps 
randomly failing. This would not be an issue if I could simply resume the 
workflow from the failed step, but it seems that this is not possible. Instead, 
I'm forced to restart the workflow from the beginning. Is this true or am I 
missing something? 

Thanks, 


Jason 






-- Jason Greenbaum, Ph.D. 
Manager, Bioinformatics Core | jgb...@liai.org 
La Jolla Institute for Allergy and Immunology 




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[galaxy-dev] specifying nodes/processors with PBS

2012-05-23 Thread J. Greenbaum

Hi, 


I've successfully configured a local galaxy instance to submit jobs to our 
local cluster, but have a question regarding setting PBS options such as the 
number of nodes and processors to use. I've followed the guide here: 

http://wiki.g2.bx.psu.edu/Admin/Config/Performance/Cluster?highlight=%28pbs%29 

Is there any way to do this in a tool-specific manner. For example, for running 
tophat to do a mapping run, I would want it to use 24 CPUs, but for simply 
converting a file type I would want only 1 CPU requested. Is there a way to 
specify this somewhere? 

Thanks, 


Jason 





-- Jason Greenbaum, Ph.D. 
Manager, Bioinformatics Core | jgb...@liai.org 
La Jolla Institute for Allergy and Immunology 




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Re: [galaxy-dev] upload from local filesysem

2012-05-23 Thread J. Greenbaum
Thanks for the reply. Ideally, I would have a cron job run that would monitor a 
directory and automatically add the new files to a particular library. I do not 
want the end users to have to deal with this. Does anyone have a working 
solution or ideas for implementation? This is the main sticking point that is 
preventing us from using galaxy for our in-house next-gen sequencing pipeline. 

Thanks, 

Jason 

-- Jason Greenbaum, Ph.D. 
Manager, Bioinformatics Core | jgb...@liai.org 
La Jolla Institute for Allergy and Immunology 

- Original Message -

 From: Alex Bossers alex.boss...@wur.nl
 To: J. Greenbaum jgb...@liai.org, galaxy-dev@lists.bx.psu.edu
 Sent: Wednesday, May 23, 2012 1:24:13 AM
 Subject: RE: [galaxy-dev] upload from local filesysem

 You can do so / allowing them to, by using the galaxy ftp solution.

 That way users can link or provide files in their email subdirs. You
 have to sort out how to set the users/rights properly on your linux
 box though.

 But see the galaxy ftp solution pages, it might be of help.

 Alex

 
 Van: galaxy-dev-boun...@lists.bx.psu.edu
 [galaxy-dev-boun...@lists.bx.psu.edu] namens J. Greenbaum
 [jgb...@liai.org]
 Verzonden: woensdag 23 mei 2012 3:01
 To: galaxy-dev@lists.bx.psu.edu
 Onderwerp: [galaxy-dev] upload from local filesysem

 It seems that this topic has been covered at least several times and
 I've found this thread to be helpful:

 http://user.list.galaxyproject.org/Speed-up-uploading-into-local-Galaxy-terribly-slow-tp4429327p4436053.html

 However, as far as I understand, this will only allow the Admin to do
 the upload from a local disk. Has anyone had success allowing a
 regular user to upload from a local disk? This seems like a common
 issue, particularly with large sequencing files. It would be great
 to be able to add this as the first step in a workflow.

 Any info would be appreciated.

 Thanks,

 Jason

 --
 Jason Greenbaum, Ph.D.
 Manager, Bioinformatics Core |
 jgb...@liai.orgmailto:jgb...@liai.org
 La Jolla Institute for Allergy and Immunology
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Re: [galaxy-dev] specifying nodes/processors with PBS

2012-05-23 Thread J. Greenbaum
Excellent! Thanks for the reply. 

-J 

-- Jason Greenbaum, Ph.D. 
Manager, Bioinformatics Core | jgb...@liai.org 
La Jolla Institute for Allergy and Immunology 

- Original Message -

 From: Anthonius deBoer thondeb...@me.com
 To: J. Greenbaum jgb...@liai.org
 Cc: galaxy-dev@lists.bx.psu.edu
 Sent: Wednesday, May 23, 2012 2:03:18 PM
 Subject: Re: [galaxy-dev] specifying nodes/processors with PBS

 Yes, you can set these things for each tool independently.
 Check the file

 universe_wsgi.ini

 and check the section:

 [galaxy:tool_runners]

 There you can set the -pe options for GSUB in the same way you can do
 on the command line...

 Here's an example of my setup:

 gatk_variant_select = drmaa://-V -pe smp 1
 gatk_validate_variants = drmaa://-V -pe smp 1
 far = drmaa://-V -pe smp 1
 velveth = drmaa://-V -pe mpi 4
 velvetg = drmaa://-V -pe mpi 8

 I set the -pe mpi option to 8 for VELVETH/G and to 1 for GATK for
 instance

 Thon
 On May 23, 2012, at 12:47 PM, J. Greenbaum jgb...@liai.org wrote:

  Hi,
 

  I've successfully configured a local galaxy instance to submit jobs
  to our local cluster, but have a question regarding setting PBS
  options such as the number of nodes and processors to use. I've
  followed the guide here:
 

  http://wiki.g2.bx.psu.edu/Admin/Config/Performance/Cluster?highlight=%28pbs%29
 

  Is there any way to do this in a tool-specific manner. For example,
  for running tophat to do a mapping run, I would want it to use 24
  CPUs, but for simply converting a file type I would want only 1 CPU
  requested. Is there a way to specify this somewhere?
 

  Thanks,
 

  Jason
 

  -- Jason Greenbaum, Ph.D.
 
  Manager, Bioinformatics Core | jgb...@liai.org
 
  La Jolla Institute for Allergy and Immunology
 

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Re: [galaxy-dev] Uploading problem

2012-05-22 Thread J. Greenbaum
Thanks for the info. It's definitely browser-related as I was having the issue 
in Chrome, but FF seems to be working... 

-J 

-- Jason Greenbaum, Ph.D. 
Manager, Bioinformatics Core | jgb...@liai.org 
La Jolla Institute for Allergy and Immunology 

- Original Message -

 From: Jorrit Boekel jorrit.boe...@scilifelab.se
 To: J. Greenbaum jgb...@liai.org, galaxy-dev@lists.bx.psu.edu
 Sent: Tuesday, May 22, 2012 7:04:26 AM
 Subject: Re: [galaxy-dev] Uploading problem

 Hi,

 Hanging uploads have been a problem before with small and large
 files:
 http://comments.gmane.org/gmane.science.biology.galaxy.devel/4583

 I don't know if it's been fixed, but it seemed to depend on browser
 choice and on what server was running. I was recommended to use the
 nginx server IIRC. I still have the occasional problem though, but
 (I hope) less often.

 cheers,
 jorrit

 On 05/22/2012 03:50 PM, J. Greenbaum wrote:
  Hi Julie, All,
 

  I have a similar problem on a test environment with a postgres
  backend. The file that I'm uploading is a 50MB GTF file and it I'm
  getting the same behavior. Any help would be appreciated.
 

  Thanks,
 

  Jason
 

  -- Jason Greenbaum, Ph.D.
 
  Manager, Bioinformatics Core | jgb...@liai.org
 
  La Jolla Institute for Allergy and Immunology
 

  - Original Message -
 

   From: julie dubois dubju...@gmail.com
  
 
   To: galaxy-dev@lists.bx.psu.edu
  
 
   Sent: Tuesday, May 22, 2012 12:12:06 AM
  
 
   Subject: [galaxy-dev] Uploading problem
  
 

   Hi,
  
 
   I test a production environnement of Galaxy with apache proxy and
   mysql database.
  
 
   It work, but when I upload a file (it's not very big : 460 Mo),
   Galaxy says that dataset is uploading and it has runned since
   yesterday and it's not finish!
  
 
   Where can the problem be ? Is it an installation problem or a
   material limitation : it's run in a machine with 8 Go RAM and 4
   cores processor.
  
 

   Thank you
  
 

   Julie
  
 

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[galaxy-dev] upload from local filesysem

2012-05-22 Thread J. Greenbaum

It seems that this topic has been covered at least several times and I've found 
this thread to be helpful: 

http://user.list.galaxyproject.org/Speed-up-uploading-into-local-Galaxy-terribly-slow-tp4429327p4436053.html
 





However, as far as I understand, this will only allow the Admin to do the 
upload from a local disk. Has anyone had success allowing a regular user to 
upload from a local disk? This seems like a common issue, particularly with 
large sequencing files. It would be great to be able to add this as the first 
step in a workflow. 


Any info would be appreciated. 


Thanks, 


Jason 


-- Jason Greenbaum, Ph.D. 
Manager, Bioinformatics Core | jgb...@liai.org 
La Jolla Institute for Allergy and Immunology 




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Please keep all replies on the list by using reply all
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