[galaxy-dev] GATK Unified Genotyper Error

2012-08-10 Thread Sarah Diehl
Hello all, one of my users encounters a strange error with the GATK tool "Unified Genotyper". At the end of the message you see the original error report. The problem there was that the reference genome is missing. The tool was run as a part of a bigger workflow, but I double checked the workfl

Re: [galaxy-dev] GATK unified genotyper

2011-09-29 Thread Daniel Blankenberg
Hi Ilya, Something similar to this is planned, but directly referencing location files is being deprecated in favor of using the tool_data_tables mechanism. The validator implementation does not yet support data tables, but will be enhanced in the future. Additionally, the selection functional

Re: [galaxy-dev] GATK unified genotyper

2011-09-28 Thread Chorny, Ilya
To prevent user error in mixing genomes, would you be interested in a feature in which the unified genotyper choses the genome in a fashion similar to how samtools know which which genome to use? Ilya Chorny Ph.D. Bioinformatics Scientist I Illum

Re: [galaxy-dev] GATK Unified Genotyper

2011-09-16 Thread Daniel Blankenberg
Hi Ilya, --genotype_likelihoods_model / -glm is available under advanced options for the tool; it can be set to both, snp or indel. This parameter might be pulled out from the advanced options heading and placed on the base options in the future to make it easier to access. Thanks for the sugg

Re: [galaxy-dev] GATK Unified Genotyper

2011-09-09 Thread Chorny, Ilya
The newest version of the Unified Genotyper has a -glm option which needs to be set to both to call indels. BTW, any interest in writing a wrapper for the Depth of Coverage tool? Thanks, Ilya From: Daniel Blankenberg [mailto:d...@bx.psu.edu] Sent: Wednesday, September 07, 2011 10:57 AM To: Ch

Re: [galaxy-dev] GATK Unified Genotyper

2011-09-07 Thread Daniel Blankenberg
Hi Ilya, The JVM can be quite a pain when determining the best way to determine max memory, however the underlying gatk_wrapper.py script was updated last week in 5970:20215fcf6da7 to allow using -Xmx or XX:DefaultMaxRAMFraction. Each of the GATK tools are currently already set to use 'XX:Defau

Re: [galaxy-dev] GATK Unified Genotyper

2011-09-07 Thread Chorny, Ilya
Hi Daniel, I have been implementing the GATK unified genotyper and I was having some issues. Occasionally I would get an error that the Java virtual machine would not start. I got around that by adding the Xmx3g parameter to the command line. I also added the -nt 3 because otherwise it's really