Re: [galaxy-dev] samtools: command not found in bowtie2

2014-11-26 Thread Anna Terry
great, thanks On 26 November 2014 at 16:39, bjoern.gruen...@googlemail.com < bjoern.gruen...@gmail.com> wrote: > I would use .bashrc. You can also try to symlib the default dir to the > actual /bin directory. Maybe this works for you. > > 2014-11-26 17:36 GMT+01:00 Anna Terry : > >> is bash_profi

Re: [galaxy-dev] samtools: command not found in bowtie2

2014-11-26 Thread bjoern.gruen...@googlemail.com
I would use .bashrc. You can also try to symlib the default dir to the actual /bin directory. Maybe this works for you. 2014-11-26 17:36 GMT+01:00 Anna Terry : > is bash_profile the best place for this, or is somewhere else better? > > Cheers > Anna > > On 26 November 2014 at 16:29, bjoern.gruen.

Re: [galaxy-dev] samtools: command not found in bowtie2

2014-11-26 Thread Anna Terry
is bash_profile the best place for this, or is somewhere else better? Cheers Anna On 26 November 2014 at 16:29, bjoern.gruen...@googlemail.com < bjoern.gruen...@gmail.com> wrote: > The problem is that bowtie and Galaxy (can) use a different samtools > version. Galaxy needs samtools to create met

Re: [galaxy-dev] samtools: command not found in bowtie2

2014-11-26 Thread bjoern.gruen...@googlemail.com
The problem is that bowtie and Galaxy (can) use a different samtools version. Galaxy needs samtools to create metadata for BAM files, so this process is actually running ofter bowtie has finished. You need a samtools version globally accessible for Galaxy. Make sure you have smatools in your $PATH

Re: [galaxy-dev] samtools: command not found in bowtie2

2014-11-26 Thread Anna Terry
I just tried still the same problem. I ran bowtie2 to test, again I saw samtools come up on top, but same error: Traceback (most recent call last): File "/home/galaxy/galaxy-dist/lib/galaxy/jobs/runners/local.py", line 129, in queue_job job_wrapper.finish( stdout, stderr, exit_code ) File

Re: [galaxy-dev] samtools: command not found in bowtie2

2014-11-26 Thread Eric Kuyt
​Could you try going to Admin -> Manage Installed tool shed repositories uninstall -> reinstall ​package_samtools_0_1_18 On 26 November 2014 at 16:55, Anna Terry wrote: > yway, I am also getting the problem with samtools filter sam or bam -- Central Veterinary Institute of Wageningen UR

Re: [galaxy-dev] samtools: command not found in bowtie2

2014-11-26 Thread Anna Terry
What is really weird is when running the bowtie2 tool, before it quits, I can see samtools come up in top On 26 November 2014 at 15:55, Anna Terry wrote: > So I actually still have a problem, > (I guess the setting was still there somewhere from the bash_profile) > > Anyway, I am also getting th

Re: [galaxy-dev] samtools: command not found in bowtie2

2014-11-26 Thread Anna Terry
So I actually still have a problem, (I guess the setting was still there somewhere from the bash_profile) Anyway, I am also getting the problem with samtools filter sam or bam Traceback (most recent call last): File "/home/galaxy/galaxy-dist/lib/galaxy/jobs/runners/local.py", line 129, in queue

Re: [galaxy-dev] samtools: command not found in bowtie2

2014-11-26 Thread Björn Grüning
Hi Anna, you need to install samtools into your system or via the toolshed. If you install it via the toolshed you need to create a symlink. Lets assume you have samtools installed in: ~/tool_deps/samtools/0.19/iuc/2828727744/ than you need to create a symlink from ~/tool_deps/samtools/0.19/iuc

Re: [galaxy-dev] samtools: command not found in bowtie2

2014-11-26 Thread Eric Kuyt
Hi Anna, Setting your own path variables shouldn't be necessary for toolshed tools, so probably something is wrong with the installation. could you post your tooldependencies/bowtie2/2.1.0/devteam/package_bowtie2_2_1_0/017a00c265f1/INSTALLATION.log file? On 26 November 2014 at 13:49, Anna Terry

[galaxy-dev] samtools: command not found in bowtie2

2014-11-26 Thread Anna Terry
Hi, I installed bowtie2 from the toolshed, but get the following error when I run it: Traceback (most recent call last): File "/home/galaxy/galaxy-dist/lib/galaxy/jobs/runners/local.py", line 129, in queue_job job_wrapper.finish( stdout, stderr, exit_code ) File "/home/galaxy/galaxy-dist/l