Re: [galaxy-user] Lastz

2011-11-11 Thread Nate Coraor
On Nov 11, 2011, at 9:21 AM, Andrew South wrote: Hello folks Anyone else having trouble with running Lastz to map? Jobs are being sent but not running. It stopped working for me two days ago after working perfectly, I've tried fiddling with the formats but no joy. Hi Andy, It

[galaxy-user] Cufflinks Annotation problem

2011-11-11 Thread Bidwell, Christopher A.
Colleagues, I am having trouble running cufflinks with an annotation file on the public Galaxy. The assembled transcripts gtf file has all the FPKM at 0 although the gene expression and transcript expression tab files have values for FPKM. I have seen the SEQanswers threads about the

Re: [galaxy-user] Lastz

2011-11-11 Thread Nate Coraor
On Nov 11, 2011, at 10:01 AM, Andrew South wrote: Thanks Nate, hope it's a quick fix. Best wishes, Andy Hi Andy, All backlogged NGS jobs should now be running, new ones will likely queue until free slots are available. Please let us know if there's further trouble, and thanks for using

[galaxy-user] MA2C peak calling or nimbelgen chip on chip analysis

2011-11-11 Thread shamsher jagat
Do we have option of running MA2C peaks in Galaxy or is tehre an option of analyzing Chip on Chip data from Nimbelgen. Thanks ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public

Re: [galaxy-user] Cufflinks Annotation problem

2011-11-11 Thread Jennifer Jackson
Hi Chris, Clearing the incorrect database assignment can be done by clicking on the pencil icon for the dataset, and in the database pull down menu, select the list header: - Additional Species Are Below - As you mention, chromosome names need to be identical between the

[galaxy-user] Trouble in extracting species on maf imported from UCSC

2011-11-11 Thread 4urelie K
Hi, When I import a multiz46way maf alignement from UCSC, if is is a whole chromosome, then when I try to Filter MAF blocks by Species the list of species does not appear. If I import a small region, it works... Is it a question of size? Although it seems OK when I look at it, could it be a

Re: [galaxy-user] BWA question on Mac OS X 10.7.2

2011-11-11 Thread Jennifer Jackson
Hi Chris, MAC OS doesn't come with a compiler, you need to get Xcode from the Apple store and install to have gcc. This is free. http://developer.apple.com/xcode For help beyond that with BWA itself, the tool authors are the best source for help. http://sourceforge.net/projects/bio-bwa/

Re: [galaxy-user] Trouble in extracting species on maf imported from UCSC

2011-11-11 Thread Jennifer Jackson
Hi Aurelie, Would you have time to share a history link containing the two types of datasets for me? Use Options - Share or Publish, generate the link, then email it back to me directly. Please note the dataset(s) with the issue if not obvious (or make a note about it in the info field of the

Re: [galaxy-user] MA2C peak calling or nimbelgen chip on chip analysis

2011-11-11 Thread Jennifer Jackson
Hello Shamsher, The tool group NGS: Peak Calling will be of interest. Each tool has some basic documentation plus links out to associated publications and tool author documentation resources. And a tutorial covering ChIP-seq analysis using MACS is in our Shared Data - Published Pages -

[galaxy-user] Adding new data source

2011-11-11 Thread Joachim Baran
Hello! I have extended a service to support asynchronous data depositing (http://wiki.g2.bx.psu.edu/Admin/Internals/Data%20Sources), but I cannot quite figure out how to access it from the Galaxy web-site. Can someone point me to the right documentation for integrating the service into