[galaxy-user] RE : Error out of memory when trying to retrieve output

2013-08-30 Thread Delong, Zhou
Hello, This solved my problem. Thanks. Delong De : Dannon Baker [dannon.ba...@gmail.com] Envoyé : 29 août 2013 16:07 À : Delong, Zhou Cc : galaxy-u...@bx.psu.edu Objet : Re: [galaxy-user] Error out of memory when trying to retrieve output Do you have debug

[galaxy-user] Which Input FASTQ quality scores type should I choose when run FASTQ Groomer?

2013-08-30 Thread Du, Jianguang
Hi All, I downloaded some RNA-seq datasets from NCBI. The datasets were generated by Illumina Hiseq 2000. I am not sure which Input FASTQ quality scores type I should choose when run FASTQ Groomer. Below shows the scores of 2 reads of a dataset, I renamed them as read 1 and read 2. 1)

[galaxy-user] Barcode Splitter Problem

2013-08-30 Thread Priyanka Vengurlekar
Hi all, I need some serious help i got output from the Miseq machine in fastq file format. My supevisor asked me to separate barcodes, so since monday i have been struggling to use this in command- line and executed it but either there is some mistake that it doesnt recognize any barcodes at

Re: [galaxy-user] watching command line to a query

2013-08-30 Thread Maddhi, Srinivas
Hi Jen, This would be very useful for users who don't have access to Galaxy logs. Could you please describe in additional detail how (sequence of steps) to cause a tool to fail in order to make the bug icon appear ? I am assuming you can, through the method you suggested, cause a tool/job

Re: [galaxy-user] Barcode Splitter Problem

2013-08-30 Thread Jennifer Jackson
Hi Piyu, Sorry to hear that you are having problems. The barcode splitter tools requires two inputs and each must be labeled correctly when using the tool in the UI (datatype assignment - using pencil icon or at upload). Binaray/compressed files are not permitted in the Galaxy UI (or when

Re: [galaxy-user] Which Input FASTQ quality scores type should I choose when run FASTQ Groomer?

2013-08-30 Thread Jennifer Jackson
Hi Jianguang, I agree - already Sanger Phred +33 offset quality scores, meaning you want datatype .fastqsanger (with near certainty). To double check, take a sample and run FastQC on it to be exact, or run this tool on the entire dataset if you plan on doing quality checks anyway (potential