[galaxy-user] Gene Name in Cufflink/compare/diff

2011-01-24 Thread Matteo Bovolenta
Hi all, when I run a RNASeq analysis using tophat, cufflink, coffcompare and cuffdiff by aligning my data to the RefSeq genes I obtain tables from cufflink/compare/diff which does not include the gene name, but only the NM_. Does someone knows how I can obtain all the tables with the gene name? T

[galaxy-user] An update to Galaxy CloudMan

2011-01-24 Thread Enis Afgan
We'd like to announce an update to Galaxy CloudMan - a completely standalone version Galaxy on Amazon's cloud. The new version has been released that brings a number of improvements to the core functionality of CloudMan and expands the number of tools available within Galaxy CloudMan to match that

[galaxy-user] NGS RNA-seq

2011-01-24 Thread Slim Sassi
Hello, I have followed the steps described by Jeremy in his exercise (http://main.g2.bx.psu.edu/u/jeremy/p/galaxy-rna-seq-analysis-exercise) to analyze RNA-seq data and all of the steps seemed to go ok except that at the end the gene IDs were missing, so for example the file CDS diff file had n

Re: [galaxy-user] Run tools/Workflow from command line

2011-01-24 Thread Jennifer Jackson
Hello Sonali, More API help can be found in the README and example scripts in the source code location: galaxy-dist/scripts/api. We have plans to incorporate more of this into the wiki soon, but for now, this is best place to get API information. Hopefully this helps, Jen Galaxy team On 1/

Re: [galaxy-user] NGS RNA-seq

2011-01-24 Thread Jennifer Jackson
Hello Slim, Would you please share a link to your history (Options -> Share or Publish) and I can take a look? You can email me directly with the link. Best, Jen Galaxy team On 1/24/11 5:47 AM, Slim Sassi wrote: Hello, I have followed the steps described by Jeremy in his exercise (http://m

Re: [galaxy-user] Gene Name in Cufflink/compare/diff

2011-01-24 Thread vasu punj
This is a knwon issue of GTF file from Ensembl --- On Mon, 1/24/11, Matteo Bovolenta wrote: From: Matteo Bovolenta Subject: [galaxy-user] Gene Name in Cufflink/compare/diff To: galaxy-u...@bx.psu.edu Date: Monday, January 24, 2011, 5:05 AM Hi all, when I run a RNASeq analysis using tophat,

Re: [galaxy-user] Gene Name in Cufflink/compare/diff

2011-01-24 Thread Jeremy Goecks
Hi Matteo and Vasu, There are different ways to refer to genes. Names that start with NM_ are termed 'accession numbers,' and they are a valid way to refer to genes. Matteo, what you may want is the canonical gene name (e.g. Xkr4). If so, you'll want to use a gene annotation/reference file fro

Re: [galaxy-user] "interval" attribute for Input?

2011-01-24 Thread Sonali Amonkar
Hi, What I am trying is, A) to write an exec_after_process for tool1(Scatterer tool). This tool1 gives multiple outputs(in the form of files names generated by Galaxy). --

[galaxy-user] Fw: NGS RNA-seq

2011-01-24 Thread vasu punj
Thanks Jeremy,   The issue mentioned by Slim is also related to the same issue as of Matteo. In the end out put files either dont have geneIDS or has multiple enteries of particular gene. Any suggestion?     --- On Mon, 1/24/11, Slim Sassi wrote: From: Slim Sassi Subject: [galaxy-user]

Re: [galaxy-user] Gene Name in Cufflink/compare/diff

2011-01-24 Thread Rory Kirchner
For the ensembl annotation, you can download the gtf file from ensembl for your organism here: http://uswest.ensembl.org/info/data/ftp/index.html To use this, you need to fix it because the chromosome names are not correct (depending on your organism, it is not correct for mouse and rat at leas

Re: [galaxy-user] Gene Name in Cufflink/compare/diff

2011-01-24 Thread Jennifer Jackson
Hello Matteo, The UCSC Genes table knownCanonical is one source for gene names, but another choice would be the RefSeq Genes track's primary table "refGene". When extracting from the UCSC Table browser, use the output file type of "all fields from selected table". The gene name will be under

[galaxy-user] Unsubscribe

2011-01-24 Thread Ralf JAUCH
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Re: [galaxy-user] Unsubscribe

2011-01-24 Thread Jennifer Jackson
Hello - Please use this form: http://lists.bx.psu.edu/listinfo/galaxy-user Thanks! Jen Galaxy team On 1/24/11 8:01 AM, Ralf JAUCH wrote: ___ galaxy-user mailing list galaxy-user@lists.bx.psu.edu http://lists.bx.psu.edu/listinfo/galaxy-user --