Re: [galaxy-user] Request to Add Reference Genome

2011-12-06 Thread Jennifer Jackson
Hello Matt, I am not sure what you mean by an annotated genome, would you be able to send more details? Meanwhile, I can let you know that the tools that use a reference genome (custom or native) all expect a fasta formatted input and use the data directly or create indexes from the data. An

Re: [galaxy-user] [galaxy-dev] Urgent help needed

2011-12-06 Thread Dannon Baker
I don't know anything about the tool or parameters in question , but it looks like the expected output filename is well formatted enough that you can derive it from the input filename and parameters. You could write a small python wrapper to grant you full control over manipulating output files

Re: [galaxy-user] How to select line with chr1?

2011-12-06 Thread Jennifer Jackson
Hello Di, The tool "Filter and Sort -> Select" can be used to select lines from a .bed file for chr1 only. On the tool form, in text box labeled "the pattern:", enter this term: ^chr1\s Then sort with the tool "Sort" from the same tool group. Hopefully this helps, Best, Jen Galaxy team O

Re: [galaxy-user] Sort sam or bam

2011-12-06 Thread Jennifer Jackson
Hello Clare, An example of how to sort a SAM file is included in the workflow from #2 on this FAQ (it can be imported and the sort modified as needed): http://usegalaxy.org/u/jeremy/p/transcriptome-analysis-faq If you are starting with a BAM file, convert BAM->SAM, then after sorting, back wi

[galaxy-user] bed file to gene list

2011-12-06 Thread Dr Chris Futtner, Ph.D.
Can anyone point me to a good workflow to convert my ChIP-seq data intervals to gene names. My peaks fall both within genes and within intergenic areas. Thanks, Chris Christopher Futtner, Ph.D. Dept. of Surgery Duke University Durham, NC 27710 email: christopher.futt...@duke.edu phone: 919-684-11

Re: [galaxy-user] bed file to gene list

2011-12-06 Thread Jennifer Jackson
Hello, There is no general workflow, primarily because there is no single source of gene annotation and some formatting may be necessary. But once that part is resolved, using a single tool in "Operate on Genomic Intervals" can most often assign gene names to query genome regions. The first

Re: [galaxy-user] Uploading Genome from Ensembl

2011-12-06 Thread Jennifer Jackson
Hello Sheena, It is odd that this particular genome exists in two versions, with different content, for the same release number/date. Ensemble will update annotation with new releases, but not the reference genome itself unless they also increment the genome build number. The Zebrafish proje

Re: [galaxy-user] Uploading Genome from Ensembl

2011-12-06 Thread Hiram Clawson
Good Afternoon Sheena: Can you please explain what is different between the Ensembl and UCSC Zebrafish Zv9 genome sequences ? --Hiram On 11/4/11 2:50 PM, Scroggins, Sheena wrote: How do I upload the Zebrafish genome from Ensembl to my user history in Galaxy? I'm trying to map my RNA-Seq data