Research Assistant/Laboratory Manager
Atherothrombosis and Vascular Biology Laboratory
From: Joe Myers
Sent: Saturday, 2 May 2020 4:49 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC DAB
Kristy:
I’m thinking along the same lines as Paula
of the antigen, pretreatment reagent
and ‘labeling’ enzyme.
Cheers,
Joe Myers, M.S.,CT/QIHC(ASCP)
From: Kristy Castillo via Histonet
Sent: Friday, May 1, 2020 11:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC DAB
Hi Histonetters,
We are starting
Since you are newbies, I am just putting IHC 101 out there.
Permanent red is for alkaline phosphatase detection and DAB is for HRP.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com
A sharp
Thanks for the tip!
On Fri, May 1, 2020 at 8:53 AM Val Tolley wrote:
> It’s difficult to know without your full protocol, but you can easily test
> your DAB.
>
> Take a paper towel and add a drop of your secondary/HRP. Add a drop of
> DAB to that. If it doesn’t immediately turn brown, your
It’s difficult to know without your full protocol, but you can easily test your
DAB.
Take a paper towel and add a drop of your secondary/HRP. Add a drop of DAB to
that. If it doesn’t immediately turn brown, your hydrogen peroxide has likely
degraded into water. Make a fresh batch of DAB and
Hydrogen Peroxide gone bad / or not being added???
From: Kristy Castillo via Histonet
Sent: Friday, May 1, 2020 11:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC DAB
Hi Histonetters,
We are starting our IHC (fun times), we are having
Hi Histonetters,
We are starting our IHC (fun times), we are having trouble with the DAB
lighting up. Processed for 5 thru 10 minutes and still nothing. Our
permanent red is working just fine. Any ideas.
Thank you!
Kristy
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Sent: Thursday, April 4, 2013 5:23 PM
Subject: [Histonet] IHC (DAB) after HE?
Dear Histonetters,
I would like to perform IHC (DAB) after HE. Do I need to remove HE
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Subject: Re: [Histonet] IHC (DAB) after HE?
Yes, you need to remove it. Hydrate your sections and place them in a 1% aq.
acetic acid solution (it will be a weak acid solution
Of MARY T HODGES
Sent: Friday, April 05, 2013 8:44 AM
To: Rene J Buesa ; nmargar...@luriechildrens.org ;
histonet@lists.utsouthwestern.edu ; histonet-boun...@lists.utsouthwestern.edu ;
histonet-requ...@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC (DAB) after HE?
I have just ihc over
Dear Histonetters,
I would like to perform IHC (DAB) after HE. Do I need to remove HE? If answer
is YES, my question HOW? Can I ask the protocol for that?
Thanks in advance,
Naira
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No. Re-hydrating the tissue will remove most (if not all) of the eosin and the
hematoxylin will serve as your counterstain (although you may need to
counterstain again at the end of you protocol). If you use antigen retrieval
for an unstained slide, you will have to use it on the HE-stained
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