Hi David
Will do so, thank you! That makes a lot of sense. I have also added the
mzXML files but this might be the cause of the discrepancy I see!
Thanks
Shagun
On Friday, March 25, 2022 at 4:42:46 PM UTC-4 David Shteynberg wrote:
> Hello Shagun,
>
> I noticed in your condition file you are us
Hello Shagun,
I noticed in your condition file you are using TMT10 with the following
masses:
The difference between neighboring channels is <0.01 at the lowest and yet
you are using tolerance of 0.2:
I think the appropriate mass tolerance for t
Hello Shagun,
Also please include your mzML files, which contain the actual data that
Libra quantifies.
Thanks!
-David
On Fri, Mar 25, 2022 at 10:34 AM David Shteynberg <
david.shteynb...@isbscience.org> wrote:
> Please place your files on a shared drive and send me a link.
>
> On Fri, Mar 25,
Please place your files on a shared drive and send me a link.
On Fri, Mar 25, 2022 at 10:34 AM Shagun Gupta wrote:
> Hi David
>
> Could you suggest a good email to reach you with? I can share the
> pep.xml's and Libra condition file that way?
>
> -Shagun
>
> On Friday, March 25, 2022 at 11:00:38
Hi David
Could you suggest a good email to reach you with? I can share the pep.xml's
and Libra condition file that way?
-Shagun
On Friday, March 25, 2022 at 11:00:38 AM UTC-4 David Shteynberg wrote:
> Hello Shagun,
>
> Thank you for your email and interest in the TPP. I have recently been
>
Hello Shagun,
Thank you for your email and interest in the TPP. I have recently been
comparing TMTPro analysis by ProteomeDiscoverer and Byonic to that produced
by TPP's Libra. As far as I can tell, when I run and compare the
quantities (intensities) they are mostly the same between Libra (witho
Hello
Our lab has been trying to use Libra to quantify MS2 (Ion-trap)
identification and MS3-based quantification of TMT datasets and we were
trying to benchmark with an artificial yeast and mammalian spiked dataset
with known fold-change (FC) values. However we observe drastically
different v
