[spctools-discuss] Re: Maldi data protein prophet problem

2009-10-16 Thread Melody Lam
Dear David, I recently suspect the causes of this problem with the MALDI DATA option. As I am using ProbID as the search engine, and use the SEQUEST pipeline in TPP. Would this be a cause of the problem? I use the out2XML program from the ProbID package but not the TPP one. Also I found that the

[spctools-discuss] Re: Maldi data protein prophet problem

2009-10-07 Thread Melody Lam
Dear David, I have updated the search parameter in ProbID and I have uploaded the output files to ftp://ftp.systemsbiology.net/incoming/melody_lam.rar I have tried to use XTandem on this dataset and I can find about 300 proteins. Thanks a lot for your help! Regards, Melody On Oct 6, 1:10 am,

[spctools-discuss] Re: Maldi data protein prophet problem

2009-09-26 Thread Melody Lam
Hi David, I've downloaded the code and build the subversion. However, the problem still exists. Here are some debug traces attached: Key: At XX\X\.h - It means the file name involved My thoughts at indicated at the comment /* ?? */ Thanks again! Regards, Melody At

[spctools-discuss] Re: Maldi data protein prophet problem

2009-09-24 Thread Melody Lam
Hi all, Thanks a lot for you advices. Here I post the output for running the peptideProphet at the command line: C:\Inetpub\wwwroot\ISB\data\probidoutput\ipimousexinteract - Ninteract.pep.xml -p0.05 -l7 -OAFm -I2 -I3 -I4 -I5 -I6 -I7 analysis100.xml analysis125.xml analy sis150.xml

[spctools-discuss] Re: Maldi data protein prophet problem

2009-09-24 Thread dshteynberg
Hi Melody, I've tracked down and fixed the bug using the files you sent. My new code is in the sashimi subversion code repository. Maybe someone can build the PeptideProphetParser executable on windows and share that with you until a new release is made available that includes this fix.

[spctools-discuss] Re: Maldi data protein prophet problem

2009-09-22 Thread Melody Lam
Hi all! I have encountered the same problem. I am also using TPP v4.3.0 on Windows XP. The output file interact.prot.shtml and the stylesheet interact.prot.xsl files are not found in the directory of the input file interact.pep.xml. However, by manually running the following line in the command

[spctools-discuss] Re: Maldi data protein prophet problem

2009-09-22 Thread Natalie Tasman
Also, consider upgrading to TPP 4.3.1, which contains several bug fixes. -Natalie On Sep 22, 2009, at 8:32 AM, Brian Pratt wrote: If it's really the same problem, then it's not a surprise that those files don't exist, since the protXML file didn't get created, because the pepXML file

[spctools-discuss] Re: Maldi data protein prophet problem

2009-09-17 Thread YAN
Hi everyone! Here are some more supplementary information from the output screen of TPP: Command 1 c:\Inetpub\tpp-bin\ProteinProphet c:/Inetpub/wwwroot/ISB/data/ probidoutput/ipimouse/interact.pep.xml c:/Inetpub/wwwroot/ISB/data/ probidoutput/ipimouse/interact.prot.xml

[spctools-discuss] Re: Maldi data protein prophet problem

2009-09-17 Thread Brian Pratt
I'll have a quick look at this. Brian On Thu, Sep 17, 2009 at 12:13 AM, YAN ms006z...@hotmail.com wrote: Hi everyone! Here are some more supplementary information from the output screen of TPP: Command 1 c:\Inetpub\tpp-bin\ProteinProphet c:/Inetpub/wwwroot/ISB/data/

[spctools-discuss] Re: Maldi data protein prophet problem

2009-09-17 Thread Brian Pratt
It appears to have never been run though peptideProphet. I should add a bit of logic to proteinProphet to catch that and issue a more useful error message. On Thu, Sep 17, 2009 at 10:52 AM, Brian Pratt brian.pr...@insilicos.comwrote: I'll have a quick look at this. Brian On Thu, Sep 17,