Re: [spctools-discuss] X!tandem High Res K-score

2013-05-13 Thread 2kl...@gmail.com
, algorithmhrk-score/note Then I would recommend lowering the spectrum, fragment monoisotopic mass error values to something small and appropriate for hi-res data. Cheers, Mike On 5/12/2013 11:45 PM, 2kl...@gmail.com javascript: wrote: Hi All, Is the high-resolution X!Tandem k-score

[spctools-discuss] Peptide Prophet and Q-Exactive data

2013-05-23 Thread 2kl...@gmail.com
Hi, I am using the TPP to analyse Q-Exactive data using X!tandem (k-score or hrk-score) searches using similar settings that I used successfully with Orbitrap data: search against fwd+decoy database, running Xinteract with the following options:accurate mass binning, *Do not* use the NTT

Re: [spctools-discuss] PTM prophet and DiMethylation

2014-07-12 Thread 2kl...@gmail.com
Sure. Let me know what you need and I will send you the data. Best, Oded On Saturday, July 12, 2014 5:11:39 AM UTC+10, David Shteynberg wrote: Hi Oded, It is likely there is an issue with PTMProphet that doesn't recognize the modifications in your dataset. If you can share a portion of

[spctools-discuss] Re: Possible Bug in TPP 4.7.1

2014-07-28 Thread 2kl...@gmail.com
Hi, I am having the same problem on a similar setup. Thanks, OK On Tuesday, July 29, 2014 4:18:11 AM UTC+10, dsim...@vcu.edu wrote: 64-bit, Windows 7, by the way. On Friday, July 25, 2014 3:46:57 PM UTC-4, dsim...@vcu.edu wrote: I think I have found a bug in TPP v4.7 POLAR VORTEX rev 1,

[spctools-discuss] mzIDentML

2020-02-04 Thread 2kl...@gmail.com
Hi there, I would like to convert PeptideProphet pep.XML (based on Comet Search) file to mzIdentML using tpp2mzid. The command seems to work and the mzID file is generated but it is missing any fragmentation information. I tried it on both Linux (Version 5.2.1) and Win (Version 5.2.0) and the

Re: [spctools-discuss] mzIDentML

2020-02-04 Thread 2kl...@gmail.com
> > Cheers, > > Mike > > > > *From:* spctools...@googlegroups.com < > spctools...@googlegroups.com > *On Behalf Of * > 2kl...@gmail.com > *Sent:* Tuesday, February 04, 2020 1:28 PM > *To:* spctools-discuss > > *Subject:* [spctools-discuss] mzIDentML &g

[spctools-discuss] Xpress ratio in pepXML do not match those in XPressPeptideUpdateParser.cgi

2022-10-18 Thread 2kl...@gmail.com
Hi all, I am using the TPP v6.1.0 Parhelion, Build 202108271510 for analyzing dimethylation following Comet (or MSFragger) search with the light label set as fixed and the heavy mass difference as variable. Xpress settings include mass tolerance of 20 ppmת a Minimum number of chromatogram

Re: [spctools-discuss] Xpress ratio in pepXML do not match those in XPressPeptideUpdateParser.cgi

2022-11-16 Thread 2kl...@gmail.com
Dear Luis, Apologies for the delay. How can I send you the file/s? Thanks On Wednesday, November 2, 2022 at 10:15:37 PM UTC+2 2kl...@gmail.com wrote: > Hi Luis, > Sure. I don't have internet access all the time in the coming week but > will send you a download link when possible. > I

Re: [spctools-discuss] Xpress ratio in pepXML do not match those in XPressPeptideUpdateParser.cgi

2022-11-02 Thread 2kl...@gmail.com
. On Sunday, October 30, 2022 at 1:41:41 AM UTC+3 Luis wrote: > Hello, > That is quite odd; Are you able to share a pepXML file so we can > troubleshoot? You can contact me directly to arrange a file transfer. > Thanks, > --Luis > > > On Tue, Oct 18, 2022 at 12:21 PM 2kl...@gma

[spctools-discuss] Xpress-related Discrepancy between pepXML and XPressPeptideUpdateParser.

2024-01-30 Thread 2kl...@gmail.com
Hi everybody,In reference to my report from 2022 (https://groups.google.com/g/spctools-discuss/c/BHES_8aAWnA/m/d11lND0nBwAJ), we continue to observe discrepancies between the ratios displayed in the pepXML and those obtained after using the XPressPeptideUpdateParser to visualize the peaks.