Re: [spctools-discuss] Analyze peptides- error

2017-11-17 Thread Elkan Dong
I'm using the newest version of TPP, but encountered the same error when using PeptideProphet. The file name I used looks like I08-F4-SAX1.pep.xml Any solution? Thanks. Elkan 在 2017年6月20日星期二 UTC+8上午1:28:34,David Shteynberg写道: > > Hello Ada, > > One immediate problem I notice is that your

Re: [spctools-discuss] Analyze peptides- error

2017-11-17 Thread David Shteynberg
Hello Elkan, Although unlikely, the dashes in your filename could be the issue. In my email on this thread I mentioned that only alphanumerics and underscores "_" should be utilized. If that fails there is a problem with the pep.xml file itself, that you would need to troubleshoot. How was the

Re: [spctools-discuss] Analyze peptides- error

2017-11-17 Thread np . dong572
Hi David, All the xml files are generated by Comet search in command line. Ok, I will revise the file names to meet the requirements and run PeptideProphet again. Wish everything go right. Thanks. Regards, Elkan 发自我的 iPad > 在 2017年11月17日,22:47,David Shteynberg

Re: [spctools-discuss] Use of XPRESS intensities in Peptide/ProteinProphet and XPRESS settings for high-res MS TPP 5.1

2017-11-17 Thread Luis Mendoza
Hello Alejandro, We will add these columns to the PepXMLViewer for the next release; I can let you know when we have a pre-release version if you would be interested in testing it. I am not sure why the ratios are not being correctly calculated; we will have a look and try to fix as well for the

Re: [spctools-discuss] Problem with Ubiquitination tag on spectrast

2017-11-17 Thread Luis Mendoza
Hello Marine, Unfortunately, you can only find these modifications by looking at the source code of SpectraST, specifically, this file: (e.g. find "GlyGly") https://sourceforge.net/p/sashimi/code/HEAD/tree/trunk/trans_proteomic_pipeline/src/Search/SpectraST/Peptide.cpp Also, from a quick