Thanks Luis and Natalie-
The reason I can't use newer versions of TPP appears to be that my
version of XRawfile2.dll is too old. Are there any other files that
TPP requires from Sequest/XCalibur/Bioworks browser in order to do a
Sequest search in the TPP? It appears that TPP is looking for newer
versions of these files than we have, but if I know what to look for
maybe I can track them down somewhere.
We have XCalibur v1.3 and Bioworks Browser v3.0. At one point I
installed TPP V3.5 (search my name in this forum to see that
discussion) and I was unable to convert .RAW files to .mzxml files. I
would get the following readout:
Settings:
centroid scans: 0
compress scans: 0
verbose mode: 1
mzML mode: 0
mzXML mode: 1
input filename: d:\Inetpub\wwwroot\ISB\data\Kristian\2008RAW-
ICAT_1\49_1.RAW
output filename: d:\Inetpub\wwwroot\ISB\data\Kristian\2008RAW-
ICAT_1\49_1.mzXML
Unable to initialize Xcalibur 2.0 interface; falling back to 1.4
interface
Unable to initialize XCalibur 1.4 interface; check installation
hint: try running the command "regsvr32 C:\<path_to_Xcalibur_dll>
\XRawfile2.dll"
unable to interface with Thermo library
I've also been unable to use various tools developed by the MacCoss
group at UW (Hardklor, MakeMS2, etc.) and the errors always seem to
come down to an old XRawfile2.dll file that is no longer supported.
I'm in the process of trying to get an updated version of Sequest,
which will hopefully contain the newer .dll files that I need.
On Mar 13, 1:03 pm, Luis Mendoza <[email protected]> wrote:
> Hello Kristian,
> I often had this problem with the Cygwin version of our tools; it seems that
> the switch to the Windows-based binaries took care of it for me. I would
> strongly advise that you install the newest version of the TPP, at least on
> the analysis computer; not only will this problem potentially go away, but
> you will benefit from the many fixes and enhancements made to the TPP in the
> past year.
> Cheers,
> --Luis
>
> On Fri, Mar 13, 2009 at 12:33 PM, Kristian
> <[email protected]>wrote:
>
>
>
>
>
> > I have two computers running TPP, one with XCalibur/Sequest installed
> > (where I do the searches) and another where I do all downstream
> > analysis. The computer I use for downstream analysis takes a long
> > time to load pages in the TPP. For example, if I need to move to a
> > different directory when browsing files or selecting files for
> > analysis, it can take up to 2 minutes for the page to load. The pages
> > that take the longest to load are folders with upwards of 100 files in
> > them (.RAW, .mzxml. .xml. etc.), but this never takes more than a few
> > seconds on the other computer. Below is the system information for
> > both computers. Are there any obvious hardware/software issues that
> > could be responsible?
>
> > TPP version v3.4 (latest version compatible with XCalibur v1.3)
> > The problem is independent of whether I use Firefox or Internet
> > Explorer
>
> > Fast system:
> > OS Name Microsoft Windows 2000 Professional
> > Version 5.0.2195 Service Pack 4 Build 2195
> > Processor x86 Family 15 Model 2 Stepping 7 GenuineIntel ~2806 Mhz
> > BIOS Version Award Modular BIOS v6.0
> > Total Physical Memory 1.0 GB
> > Available Physical Memory 587 MB
> > Total Virtual Memory 3.5 GB
> > Available Virtual Memory 2.7 GB
> > Page File Space 2.48 GB
>
> > Slow system:
> > OS Name Microsoft Windows XP Professional
> > Version 5.1.2600 Service Pack 3 Build 2600
> > Processor x86 Family 15 Model 4 Stepping 1 GenuineIntel ~3192 Mhz
> > BIOS Version/Date Dell Inc. A04, 3/16/2005
> > SMBIOS Version 2.3
> > Total Physical Memory 1 GB
> > Available Physical Memory 476 MB
> > Total Virtual Memory 2.00 GB
> > Available Virtual Memory 1.96 GB
> > Page File Space 2.41 GB- Hide quoted text -
>
> - Show quoted text -
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