Hello Kristian,
Glad you were able to complete your analysis.  One potential thing to watch
out for when you use wildcards (i.e. the asterisk) is that you may
inadvertently include a file that you did not mean to, for example: an old
analysis file with the name interact-old.pep.xml ; this would naturally
affect your results.

As of now, there is no simple way to have Petunia get past the shell limit
on number of files/characters.  Perhaps we could add an "Analyze all pep.xml
files in this directory" button or some such.  Although in general I would
suggest using a command-line or even a script if you will be running
analyses on very large numbers of files.
Cheers,
--Luis



On Wed, Apr 22, 2009 at 12:00 PM, Kristian <[email protected]>wrote:

>
> Answered my own question. I was able to run the commands from Windows
> command line.
>
> the command >xinteract *.pep.xml analyzed all 120 of my .pep.xml
> files.
>
> I copied the command line from the petunia window to incorporate my
> ICAT ratios and run protein prophet after:
> >xinteract  -Ndesiredfilename.pep.xml -p0.05 -l7 -Oip -X-m1.0-L -A-lC-r0.5
> *.pep.xml
>
> On Apr 22, 10:13 am, Kristian <[email protected]> wrote:
> > I use petunia on a Windows machine, so there is no command line from
> > my end.  I use the default settings.  I'm not even sure what Eric's
> > post means.
> > Is there a line I can edit in one of the GUI files to get around this
> > limitation?  Or otherwise, can I enter the commands in the Windows
> > command line and get around it?
> >
> > On Apr 21, 4:26 pm, "Eric Deutsch" <[email protected]>
> > wrote:
> >
> >
> >
> > > It is likely the shell that imposes the limit.
> >
> > > When you type:
> >
> > > xinteract *.pep.xml
> >
> > > the shell expands that wildcard into the full list before feeding the
> list
> > > to xinteract. The shell has an inherent limit. xinteract does not. For
> this
> > > very reason, you can get xinteract to the globbing for you with:
> >
> > > xinteract '*.pep.xml'
> >
> > > This causes the wildcard to be passed to xinteract and it will resolve
> it
> > > for you. This has been used on over 35,000 pepXML files at once. It
> works.
> >
> > > Eric
> >
> > > > -----Original Message-----
> > > > From: [email protected] [mailto:spctools-
> > > > [email protected]] On Behalf Of Brian Pratt
> > > > Sent: Tuesday, April 21, 2009 4:11 PM
> > > > To: [email protected]
> > > > Subject: [spctools-discuss] Re: Limit on number of pep.xml files that
> can
> > > > be analyzed with XInteract?
> >
> > > > There's not meant to be a limitation - can you give an example of a
> > > > command
> > > > line that fails?
> >
> > > > -----Original Message-----
> > > > From: [email protected]
> > > > [mailto:[email protected]] On Behalf Of Kristian
> > > > Sent: Tuesday, April 21, 2009 3:00 PM
> > > > To: spctools-discuss
> > > > Subject: [spctools-discuss] Re: Limit on number of pep.xml files that
> can
> > > > be
> > > > analyzed with XInteract?
> >
> > > > I have installed 4.2.1, but the problem persists.  After a bit of
> > > > experimenting, I've found that XInteract can only handle up to 99
> > > > input *.pep.xml files at a time.
> >
> > > > The operation fails with the message "failed: Operation not
> > > > permitted".
> >
> > > > On Apr 16, 1:21 pm, Natalie Tasman <[email protected]>
> wrote:
> > > > > Hi Kristian,
> >
> > > > > Also, 4.2.1 contained many bug fixes.  I'm not sure if this would
> have
> > > > been
> > > > > addressed, but it's always most efficient for us if you can try to
> > > > replicate
> > > > > the issue on the latest version.
> >
> > > > > -Natalie
> >
> > > > > On Thu, Apr 16, 2009 at 12:42 PM, Christine Vogel
> > > > <[email protected]>wrote:
> >
> > > > > > I have had some problems before if any of the pep.xml files had
> > > > identical
> > > > > > names.  Renaming them (and paths/names within the files) solved
> that
> > > > > > problem.
> >
> > > > > > Christine
> >
> > > > > > On Thu, Apr 16, 2009 at 2:17 PM, Brian Pratt
> > > > <[email protected]>wrote:
> >
> > > > > >> There's not meant to be a limitation - can you give an example
> of a
> > > > > >> command
> > > > > >> line that fails?
> >
> > > > > >> -----Original Message-----
> > > > > >> From: [email protected]
> > > > > >> [mailto:[email protected]] On Behalf Of
> Kristian
> > > > > >> Sent: Thursday, April 16, 2009 11:56 AM
> > > > > >> To: spctools-discuss
> > > > > >> Subject: [spctools-discuss] Limit on number of pep.xml files
> that can
> > > > be
> > > > > >> analyzed with XInteract?
> >
> > > > > >> I'm using TPP 4.2.0 to analyze cleavable ICAT data from an LCQ.
>  When
> > > > > >> all replicates are done, I have 120 seperate .pep.xml files to
> > > > combine
> > > > > >> and analyze.  With earlier versions of TPP (i.e. 3.4) I could
> analyze
> > > > > >> them all at once with Xinteract, but with 4.2 the operation
> simply
> > > > > >> aborts and fails with no error message.  I can analyze the files
> 40
> > > > at
> > > > > >> a time and then combine the interact.pep files in protein
> prophet,
> > > > but
> > > > > >> I'm curious if and why there's a limitation on the number of
> input
> > > > > >> files for Xinteract.- Hide quoted text -
> >
> > > > > - Show quoted text -- Hide quoted text -
> >
> > > - Show quoted text -- Hide quoted text -
> >
> > - Show quoted text -
> >
>

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