If it's really the same problem, then it's not a surprise that those files
don't exist, since the protXML file didn't get created, because the pepXML
file never went through peptideProphet.  But that doesn't quite sound like
what you're describing - check the log to make sure of what's really going
on.

Brian

On Tue, Sep 22, 2009 at 1:02 AM, Melody Lam <[email protected]> wrote:

>
> Hi all!
>
> I have encountered the same problem. I am also using TPP v4.3.0 on
> Windows XP.
>
> The output file "interact.prot.shtml" and the stylesheet
> "interact.prot.xsl" files are not found in the directory of the input
> file "interact.pep.xml".
> However, by manually running the following line in the command prompt:
> c:\Inetpub\tpp-bin\protxml2html.pl -file directory/interact.prot.xml
> The output file interact.prot.shtml works as expected.
>
> I just wonder why throught the GUI the above command is not executed.
>
> Regards,
> Melody
>
>
> On Sep 18, 2:22 am, Brian Pratt <[email protected]> wrote:
> > It appears to have never been run though peptideProphet.
> >
> > I should add a bit of logic to proteinProphet to catch that and issue a
> more
> > useful error message.
> >
> > On Thu, Sep 17, 2009 at 10:52 AM, Brian Pratt <[email protected]
> >wrote:
> >
> > > I'll have a quick look at this.
> >
> > > Brian
> >
>  > >   On Thu, Sep 17, 2009 at 12:13 AM, YAN <[email protected]>
> wrote:
> >
> > >> Hi everyone!
> > >> Here are some more supplementary information from the output screen of
> > >> TPP:
> >
> > >>  Command 1
> >
> > >>  c:\Inetpub\tpp-bin\ProteinProphet c:/Inetpub/wwwroot/ISB/data/
> > >> probidoutput/ipimouse/interact.pep.xml c:/Inetpub/wwwroot/ISB/data/
> > >> probidoutput/ipimouse/interact.prot.xml
> >
> > >>
> --------------------------------------------------------------------------------
> >
> > >> Command FAILED
> >
> > >>  Command 2 [Thu Sep 17 14:33:13 2009]        [ Show / Hide ]
> >
> > >>  c:\Inetpub\tpp-bin\protxml2html.pl -file
> c:/Inetpub/wwwroot/ISB/data/
> > >> probidoutput/ipimouse/interact.prot.xml HTML
> >
> > >>
> --------------------------------------------------------------------------------
> >
> > >> ProteinProphet (C++) by Insilicos LLC and LabKey Software, after the
> > >> original Perl by A. Keller (TPP v4.3 JETSTREAM rev 0, Build
> > >> 200908071234 (MinGW))
> > >>  (xml input) (report Protein Length) (using degen pep info)
> > >> . . . reading in c:/Inetpub/wwwroot/ISB/data/probidoutput/ipimouse/
> > >> interact.pep.xml. . .
> > >> . . . read in 0 1+, 0 2+, 0 3+, 0 4+, 0 5+, 0 6+, 0 7+ spectra with
> > >> min prob 0.05
> > >> no data - quittingEND OUTPUT
> > >> Writing results to html file:
> c:/Inetpub/wwwroot/ISB/data/probidoutput/
> > >> ipimouse/interact.prot.html
> > >> Command FAILED
> > >> ................................................
> >
> > >> I am running TPP on Windows XP and I just wonder why the program can't
> > >> read the data from the pepxml file.
> > >> I have also uploaded the pepXML file to this group at
> > >>http://groups.google.com/group/spctools-discuss/web/yan.zip
> >
> > >> Thanks a lot!
> > >>  Yan
>
> >
>

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