Dear All: Last time, I send this message to this mail list and want to get some advice. I still do not solve the problem. Here I would like describe this problem in detail and hope someone may give me some advice and help me solve this problem.
Basically, I use SCX-HPLC/MS to separate the SILAC sample, which is labeled with isotope. I did off-line SCX-HPLC/MS and collected 30 fractions for the LC/MS. The mass spectrometry is the new version from Thermo and called Velo. After the sequest search with database, the results are very big file. All the 30 fractions search files are almost 14G even after I zipped them. That means each fraction is almost 1G. I tried to analyze one fraction with TPP software and it works. When I load 5 or more fractions, TPP will not work anymore. Does anyone have any advice how to analyze such big file size data with TPP? Is there any other way to help me to analyze all the data. The major things that I need combine all the fraction and get protein ASAP ratio. Thank you for your kind help Eileen -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Eileen Yue Sent: 04 March 2010 16:44 To: [email protected] Subject: [spctools-discuss] problem to analyze the VELO data with TPP Dear All: I tried to use TPP to analyze the SILAC data produced from Velo under Xcaliber 2.1. The sequest results for each fraction is very big and I have total 30 fraction. I tried ONE fraction with TPP and it is fine to convert the raw data into mzxml and sequest results to pepxml. When I try to analyze more fraction together such as only 5 fraction, TPP does not work. Can anyone tell me how to analyze my data and any advice for this situation? Thank you so much for your help Eileen -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en. -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
