Hi Eileen, Perhaps you could start by posting the output from a failed TPP run (just copy and paste from the TPP GUI). "Will not work" doesn't provide enough information for anyone to make a helpful observation.
Brian On Mon, Mar 8, 2010 at 3:59 PM, Eileen Yue <[email protected]> wrote: > Dear All: > Last time, I send this message to this mail list and want to get some > advice. I still do not solve the problem. Here I would like describe this > problem in detail and hope someone may give me some advice and help me solve > this problem. > > Basically, I use SCX-HPLC/MS to separate the SILAC sample, which is labeled > with isotope. I did off-line SCX-HPLC/MS and collected 30 fractions for the > LC/MS. The mass spectrometry is the new version from Thermo and called Velo. > After the sequest search with database, the results are very big file. All > the 30 fractions search files are almost 14G even after I zipped them. That > means each fraction is almost 1G. I tried to analyze one fraction with TPP > software and it works. When I load 5 or more fractions, TPP will not work > anymore. > > Does anyone have any advice how to analyze such big file size data with > TPP? Is there any other way to help me to analyze all the data. The major > things that I need combine all the fraction and get protein ASAP ratio. > > Thank you for your kind help > > Eileen > > -----Original Message----- > From: [email protected] [mailto: > [email protected]] On Behalf Of Eileen Yue > Sent: 04 March 2010 16:44 > To: [email protected] > Subject: [spctools-discuss] problem to analyze the VELO data with TPP > > Dear All: > I tried to use TPP to analyze the SILAC data produced from Velo under > Xcaliber 2.1. The sequest results for each fraction is very big and I have > total 30 fraction. I tried ONE fraction with TPP and it is fine to convert > the raw data into mzxml and sequest results to pepxml. When I try to analyze > more fraction together such as only 5 fraction, TPP does not work. Can > anyone tell me how to analyze my data and any advice for this situation? > > Thank you so much for your help > Eileen > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To post to this group, send email to [email protected]. > To unsubscribe from this group, send email to > [email protected]<spctools-discuss%[email protected]> > . > For more options, visit this group at > http://groups.google.com/group/spctools-discuss?hl=en. > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To post to this group, send email to [email protected]. > To unsubscribe from this group, send email to > [email protected]<spctools-discuss%[email protected]> > . > For more options, visit this group at > http://groups.google.com/group/spctools-discuss?hl=en. > > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
