Hi Brian: By the way, when I click the back button to previous TPP, I got the following message:
* Status as of: Wed Mar 10 14:43:43 2010 * Your commands are still running. * Click here <http://localhost/tpp-bin/tpp_gui.pl?Action=display&page=wait> to view output so far * (Click here <http://localhost/tpp-bin/tpp_gui.pl?Action=userTimeoutRecovery&page=wait> if your commands have actually completed but browser timed out) * If I click second choice (Click here <http://localhost/tpp-bin/tpp_gui.pl?Action=userTimeoutRecovery&page=wait> if your commands have actually completed but browser timed out)), I got the following information: Error Found! NOCMDFILE: Cannot open /tmp/cmd_TD8V8FITW for appending: Permission denied Can anyone tell me what is the reason for this? Thank you Eileen From: [email protected] [mailto:[email protected]] On Behalf Of Eileen Yue Sent: 10 March 2010 14:28 To: [email protected] Subject: RE: [spctools-discuss] how to analyze the big file size date with TPP Hi Brian: Thank you for your advice! In fact, after I load 5 or more fractions, the computer is so slow and I wait so long (~1hours) and then, I just got the “Internet Explorer cannot display the webpage” on the web and the TPP looks like shutting down automatically. When I click more information on the web and it shows the following information. Do you think it is due to the big size of the file that I load or other reasons? Thank you! Eileen What you can try: Diagnose Connection Problems [cid:[email protected]]<res://\\ieframe.dll/dnserror.htm> More information<javascript:expandCollapse('infoBlockID',%20true);> This problem can be caused by a variety of issues, including: * Internet connectivity has been lost. * The website is temporarily unavailable. * The Domain Name Server (DNS) is not reachable. * The Domain Name Server (DNS) does not have a listing for the website's domain. * There might be a typing error in the address. * If this is an HTTPS (secure) address, click Tools, click Internet Options, click Advanced, and check to be sure the SSL and TLS protocols are enabled under the security section. For offline users You can still view subscribed feeds and some recently viewed webpages. To view subscribed feeds 1. Click the Favorites Center button [cid:[email protected]] , click Feeds, and then click the feed you want to view. To view recently visited webpages (might not work on all pages) 1. Click Tools [cid:[email protected]] , and then click Work Offline. 2. Click the Favorites Center button [cid:[email protected]] , click History, and then click the page you want to view. From: [email protected] [mailto:[email protected]] On Behalf Of Brian Pratt Sent: 08 March 2010 16:16 To: [email protected] Subject: Re: [spctools-discuss] how to analyze the big file size date with TPP Hi Eileen, Perhaps you could start by posting the output from a failed TPP run (just copy and paste from the TPP GUI). "Will not work" doesn't provide enough information for anyone to make a helpful observation. Brian On Mon, Mar 8, 2010 at 3:59 PM, Eileen Yue <[email protected]<mailto:[email protected]>> wrote: Dear All: Last time, I send this message to this mail list and want to get some advice. I still do not solve the problem. Here I would like describe this problem in detail and hope someone may give me some advice and help me solve this problem. Basically, I use SCX-HPLC/MS to separate the SILAC sample, which is labeled with isotope. I did off-line SCX-HPLC/MS and collected 30 fractions for the LC/MS. The mass spectrometry is the new version from Thermo and called Velo. After the sequest search with database, the results are very big file. All the 30 fractions search files are almost 14G even after I zipped them. That means each fraction is almost 1G. I tried to analyze one fraction with TPP software and it works. When I load 5 or more fractions, TPP will not work anymore. Does anyone have any advice how to analyze such big file size data with TPP? Is there any other way to help me to analyze all the data. The major things that I need combine all the fraction and get protein ASAP ratio. Thank you for your kind help Eileen -----Original Message----- From: [email protected]<mailto:[email protected]> [mailto:[email protected]<mailto:[email protected]>] On Behalf Of Eileen Yue Sent: 04 March 2010 16:44 To: [email protected]<mailto:[email protected]> Subject: [spctools-discuss] problem to analyze the VELO data with TPP Dear All: I tried to use TPP to analyze the SILAC data produced from Velo under Xcaliber 2.1. The sequest results for each fraction is very big and I have total 30 fraction. I tried ONE fraction with TPP and it is fine to convert the raw data into mzxml and sequest results to pepxml. When I try to analyze more fraction together such as only 5 fraction, TPP does not work. Can anyone tell me how to analyze my data and any advice for this situation? Thank you so much for your help Eileen -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]<mailto:[email protected]>. To unsubscribe from this group, send email to [email protected]<mailto:spctools-discuss%[email protected]>. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en. -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]<mailto:[email protected]>. To unsubscribe from this group, send email to [email protected]<mailto:spctools-discuss%[email protected]>. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en. -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en. -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en. -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
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