Hi all, I posted my questions yesterday, but they were a bit too long to read, so I shortened it (still seems long).
I am trying to use TPP (4.3.1) to run some cICAT data I collected a while ago. All these cICAT data have been searched by Mascot (I have all the .dat files). So far I have not got my data run by TPP on my computer. Here are some questions. 1. If I have 1 mzXML file, a Mascot.dat file, and a database.fasta file, are they enough to run TPP? (I guess so). 2. Does the database.fasta file has to be the exactly same database.fasta file when I did Mascot searches (my data are a bit old). I use SwissProt database. As I recall, when you update it, the newer database overwrite the older one. So, can I use the Mascot.dat files (searched against a older version SwissProt database) along with the current database (newer version) to get TPP run? If not, where can I download those older databases? I can not find them online. 3. I redid Mascot search anyway. With this new .dat file and the SwissProt database.fasta file (for generating this .dat file), the TPP still did not run. I got following message: c:/Inetpub/wwwroot/ISB/data/SSM4-1/F030337.pep.xml [ Unreadable! ] command "c:\Inetpub\tpp-bin\Mascot2XML c:/Inetpub/wwwroot/ISB/data/ SSM4-1/F030337.dat -Dc:/Inetpub/wwwroot/ISB/data/SwissProt_57.12.fasta -Etrypsin" failed: Operation not permitted Command FAILED I do not know how to fix it. It seems some permission issue? I looked up a lot of old posts, seems no one ever mentioned this problem. Maybe it is too simple to ask? Any suggestion? Thanks a lot! Fay -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
